메뉴 건너뛰기




Volumn 284, Issue 5411, 1999, Pages 147-151

Two distinct cytokines released from a human aminoacyl-tRNA synthetase

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID TRANSFER RNA LIGASE; CYTOKINE; TYROSINE TRANSFER RNA LIGASE;

EID: 0033515887     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5411.147     Document Type: Article
Times cited : (438)

References (47)
  • 3
    • 0025996404 scopus 로고
    • C. Cerini et al., EMBO J. 10, 4267 (1991); M. Lazard and M. Mirande, Gene 132, 237 (1993); K. Shiba et al., Proc. Natl. Acad. Sci. U.S.A. 91, 7435 (1994); S. B. Rho et al., ibid. 93, 10128 (1996); F. Agou and M. Mirande, Eur. J. Biochem. 243, 259 (1997); S. B. Rho et al., J. Biol. Chem. 273, 11267 (1998).
    • (1991) EMBO J. , vol.10 , pp. 4267
    • Cerini, C.1
  • 4
    • 0027423060 scopus 로고
    • C. Cerini et al., EMBO J. 10, 4267 (1991); M. Lazard and M. Mirande, Gene 132, 237 (1993); K. Shiba et al., Proc. Natl. Acad. Sci. U.S.A. 91, 7435 (1994); S. B. Rho et al., ibid. 93, 10128 (1996); F. Agou and M. Mirande, Eur. J. Biochem. 243, 259 (1997); S. B. Rho et al., J. Biol. Chem. 273, 11267 (1998).
    • (1993) Gene , vol.132 , pp. 237
    • Lazard, M.1    Mirande, M.2
  • 5
    • 0028108750 scopus 로고
    • C. Cerini et al., EMBO J. 10, 4267 (1991); M. Lazard and M. Mirande, Gene 132, 237 (1993); K. Shiba et al., Proc. Natl. Acad. Sci. U.S.A. 91, 7435 (1994); S. B. Rho et al., ibid. 93, 10128 (1996); F. Agou and M. Mirande, Eur. J. Biochem. 243, 259 (1997); S. B. Rho et al., J. Biol. Chem. 273, 11267 (1998).
    • (1994) Proc. Natl. Acad. Sci. U.S.A. , vol.91 , pp. 7435
    • Shiba, K.1
  • 6
    • 0000983324 scopus 로고    scopus 로고
    • C. Cerini et al., EMBO J. 10, 4267 (1991); M. Lazard and M. Mirande, Gene 132, 237 (1993); K. Shiba et al., Proc. Natl. Acad. Sci. U.S.A. 91, 7435 (1994); S. B. Rho et al., ibid. 93, 10128 (1996); F. Agou and M. Mirande, Eur. J. Biochem. 243, 259 (1997); S. B. Rho et al., J. Biol. Chem. 273, 11267 (1998).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 10128
    • Rho, S.B.1
  • 7
    • 0031019325 scopus 로고    scopus 로고
    • C. Cerini et al., EMBO J. 10, 4267 (1991); M. Lazard and M. Mirande, Gene 132, 237 (1993); K. Shiba et al., Proc. Natl. Acad. Sci. U.S.A. 91, 7435 (1994); S. B. Rho et al., ibid. 93, 10128 (1996); F. Agou and M. Mirande, Eur. J. Biochem. 243, 259 (1997); S. B. Rho et al., J. Biol. Chem. 273, 11267 (1998).
    • (1997) Eur. J. Biochem. , vol.243 , pp. 259
    • Agou, F.1    Mirande, M.2
  • 8
    • 0000781162 scopus 로고    scopus 로고
    • C. Cerini et al., EMBO J. 10, 4267 (1991); M. Lazard and M. Mirande, Gene 132, 237 (1993); K. Shiba et al., Proc. Natl. Acad. Sci. U.S.A. 91, 7435 (1994); S. B. Rho et al., ibid. 93, 10128 (1996); F. Agou and M. Mirande, Eur. J. Biochem. 243, 259 (1997); S. B. Rho et al., J. Biol. Chem. 273, 11267 (1998).
    • (1998) J. Biol. Chem. , vol.273 , pp. 11267
    • Rho, S.B.1
  • 12
    • 0024997246 scopus 로고
    • Human full-length and mini TyrRSs, the extra COOH-terminal domain of human TyrRS, E. coli TyrRS, and human mature EMAP II (with a COOH-terminal tag of six histidine residues) were over-expressed in E. coli strain BL 21 (DE 3) (Novagen, Madison, WI) by induction with isopropyl β-D-thiogalactopyranoside for 4 hours. Proteins were purified on a nickel affinity column (His®Bind resin; Novagen) from the supernatant of lysed cells. Endotoxin was removed from the protein solutions by phase separation using Triton X-114 [Y. Aida and M. J. Pabst, J. Immunol. Methods 132, 191 (1990); S. Liu et al., Clin. Biochem. 30, 455 (1997).] and was determined to be <0.01 endotoxin units per milliliter by a Limulus Amebocyte Lysate gel-clot assay (E-Toxate; Sigma, St. Louis, MO).
    • (1990) J. Immunol. Methods , vol.132 , pp. 191
    • Aida, Y.1    Pabst, M.J.2
  • 13
    • 0030882663 scopus 로고    scopus 로고
    • Human full-length and mini TyrRSs, the extra COOH-terminal domain of human TyrRS, E. coli TyrRS, and human mature EMAP II (with a COOH-terminal tag of six histidine residues) were over-expressed in E. coli strain BL 21 (DE 3) (Novagen, Madison, WI) by induction with isopropyl β-D-thiogalactopyranoside for 4 hours. Proteins were purified on a nickel affinity column (His®Bind resin; Novagen) from the supernatant of lysed cells. Endotoxin was removed from the protein solutions by phase separation using Triton X-114 [Y. Aida and M. J. Pabst, J. Immunol. Methods 132, 191 (1990); S. Liu et al., Clin. Biochem. 30, 455 (1997).] and was determined to be <0.01 endotoxin units per milliliter by a Limulus Amebocyte Lysate gel-clot assay (E-Toxate; Sigma, St. Louis, MO).
    • (1997) Clin. Biochem. , vol.30 , pp. 455
    • Liu, S.1
  • 15
    • 0344866734 scopus 로고    scopus 로고
    • unpublished observations, [supplementary data can be found at the Science Web site]
    • K. Wakasugi and P. Schimmel, unpublished observations, [supplementary data can be found at the Science Web site (www.sciencemag.org/feature/data/ 990263.shl)]
    • Wakasugi, K.1    Schimmel, P.2
  • 16
    • 0026781869 scopus 로고    scopus 로고
    • J. Kao et al., J. Biol. Chem. 267, 20239 (1992); J. Kao et al., ibid. 269, 9774 (1994); J. Kao et al., ibid., p. 25106.
    • (1992) J. Biol. Chem. , vol.267 , pp. 20239
    • Kao, J.1
  • 17
    • 0028361971 scopus 로고
    • J. Kao et al., J. Biol. Chem. 267, 20239 (1992); J. Kao et al., ibid. 269, 9774 (1994); J. Kao et al., ibid., p. 25106.
    • (1994) J. Biol. Chem. , vol.269 , pp. 9774
    • Kao, J.1
  • 18
    • 0026781869 scopus 로고    scopus 로고
    • J. Kao et al., J. Biol. Chem. 267, 20239 (1992); J. Kao et al., ibid. 269, 9774 (1994); J. Kao et al., ibid., p. 25106.
    • J. Biol. Chem. , pp. 25106
    • Kao, J.1
  • 19
    • 0031214350 scopus 로고    scopus 로고
    • M. P. R. Tas et al., Cytokine 9, 535 (1997).
    • (1997) Cytokine , vol.9 , pp. 535
    • Tas, M.P.R.1
  • 20
    • 0345297975 scopus 로고    scopus 로고
    • note
    • Human MPs and PMNs were prepared from acid citrate dextrose - treated:blood of normal healthy volunteers (8).
  • 23
    • 0026643195 scopus 로고
    • M. Baggiolini and I. Clark-Lewis, FEBS Lett. 307, 97 (1992); R. C. Hoch, I. U. Schraufstätter, C. G. Cochrane, J. Lab. Clin. Med. 128, 134 (1996); M. Baggiolini, B. Dewald, B. Moser, Annu. Rev. Immunol. 15, 675 (1997).
    • (1992) FEBS Lett. , vol.307 , pp. 97
    • Baggiolini, M.1    Clark-Lewis, I.2
  • 24
    • 0030204636 scopus 로고    scopus 로고
    • M. Baggiolini and I. Clark-Lewis, FEBS Lett. 307, 97 (1992); R. C. Hoch, I. U. Schraufstätter, C. G. Cochrane, J. Lab. Clin. Med. 128, 134 (1996); M. Baggiolini, B. Dewald, B. Moser, Annu. Rev. Immunol. 15, 675 (1997).
    • (1996) J. Lab. Clin. Med. , vol.128 , pp. 134
    • Hoch, R.C.1    Schraufstätter, I.U.2    Cochrane, C.G.3
  • 25
    • 0030938510 scopus 로고    scopus 로고
    • M. Baggiolini and I. Clark-Lewis, FEBS Lett. 307, 97 (1992); R. C. Hoch, I. U. Schraufstätter, C. G. Cochrane, J. Lab. Clin. Med. 128, 134 (1996); M. Baggiolini, B. Dewald, B. Moser, Annu. Rev. Immunol. 15, 675 (1997).
    • (1997) Annu. Rev. Immunol. , vol.15 , pp. 675
    • Baggiolini, M.1    Dewald, B.2    Moser, B.3
  • 26
    • 0026005126 scopus 로고
    • C. A. Hébert, R. V. Vitangcol, J. B. Baker, J. Biol. Chem. 266, 18989 (1991); I. Clark-Lewis, C. Schumacher, M. Baggiolini, B. Moser, ibid., p. 23128; B. Moser et al., ibid. 268, 7125 (1993).
    • (1991) J. Biol. Chem. , vol.266 , pp. 18989
    • Hébert, C.A.1    Vitangcol, R.V.2    Baker, J.B.3
  • 28
    • 0027478027 scopus 로고
    • C. A. Hébert, R. V. Vitangcol, J. B. Baker, J. Biol. Chem. 266, 18989 (1991); I. Clark-Lewis, C. Schumacher, M. Baggiolini, B. Moser, ibid., p. 23128; B. Moser et al., ibid. 268, 7125 (1993).
    • (1993) J. Biol. Chem. , vol.268 , pp. 7125
    • Moser, B.1
  • 29
    • 0344434933 scopus 로고    scopus 로고
    • note
    • 125I was performed by Research & Diagnostic Antibody (Richmond, CA).
  • 30
    • 0025900818 scopus 로고
    • 125I-human mini TyrRS (specific activity of ∼60 Ci/ mmol) in the absence or presence of a 200-fold molar excess of either unlabeled TyrRSs, human mature EMAP II, human recombinant IL-8 (Calbiochem, La Jolla, CA), human recombinant Groα (Biosource International, Camaritlo, CA), or human recombinant NAP-2 (Biosource International). Cells were separated from unbound radioactivity by centrifugation at approximately 8000g for 2 min through 500 μl of a 10% sucrose/phosphate-buffered saline (PBS) cushion. The supernatant was aspirated, and the cell sediment was resuspended by using Ecolite (ICN Biomedicals, Irvine, CA) and analyzed in a scintillation counter.
    • (1991) J. Biol. Chem. , vol.266 , pp. 10666
    • Moser, B.1    Schumacher, C.2    Von Tscharner, V.3    Clark-Lewis, I.4    Baggiolini, M.5
  • 31
    • 0028222920 scopus 로고
    • 125I-human mini TyrRS (specific activity of ∼60 Ci/ mmol) in the absence or presence of a 200-fold molar excess of either unlabeled TyrRSs, human mature EMAP II, human recombinant IL-8 (Calbiochem, La Jolla, CA), human recombinant Groα (Biosource International, Camaritlo, CA), or human recombinant NAP-2 (Biosource International). Cells were separated from unbound radioactivity by centrifugation at approximately 8000g for 2 min through 500 μl of a 10% sucrose/phosphate-buffered saline (PBS) cushion. The supernatant was aspirated, and the cell sediment was resuspended by using Ecolite (ICN Biomedicals, Irvine, CA) and analyzed in a scintillation counter.
    • (1994) J. Immunol. , vol.152 , pp. 2467
    • Petersen, F.1    Flad, H.D.2    Brandt, E.3
  • 32
  • 35
    • 0344866732 scopus 로고    scopus 로고
    • note
    • 6 cells) were cultured in RPMI 1640 medium without or with heat-treated fetal bovine serum (10%). Cells were washed twice with ice-cold PBS, and resuspended in lysis buffer containing 25 mM Hepes (pH 7.5), 5 mM EDTA, 5 mM dithiothreitol, 0.1% CHAPS detergent, 2 mM phenylmethylsulfonyl fluoride (PMSF), 10 μg/ml aprotinin, 20 μg/ml leupeptin, and 10 μg/ml pepstatin A. The cells were frozen and thawed three times in liquid nitrogen, and then centrifuged for 30 min at 4°C. For examination of proteins in the cell supernatant, 20 ml of spent culture medium (to which the following protease inhibitors were added: 2 mM PMSF, 10 μg/ml aprotinin, 20 μg/ml leupeptin, and 10 μg/ml pepstatin A) was concentrated in a Centriprep-10 (Amicon, Beverly, MA), and the samples were then analyzed on 12.5% SDS-polyacrylamide gels. After transfer onto Immobilon-P transfer membrane (Millipore, Bedford, MA), the membranes were blocked with PBS and 3% BSA, and incubated with rabbit polyclonal antibodies against human full-length TyrRS. Washed membranes were then incubated with a horseradish peroxidase-linked antibody to rabbit immunoglobulin (1:4000 dilution; Amersham Life Science, Arlington Heights, IL) for detection of TyrRS.
  • 36
    • 0344434935 scopus 로고    scopus 로고
    • note
    • LDH activities were determined spectrophotometrically with a CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega, Madison, WI).
  • 44
  • 46
    • 0344434929 scopus 로고    scopus 로고
    • note
    • Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser, T, Thr; V, Val; W, Trp; and Y, Tyr.
  • 47
    • 0344866730 scopus 로고    scopus 로고
    • note
    • We thank I. U. Schraufstātter for RBL2H3 rat basophilic leukemia cells expressing IL-8 type A or B receptor, and D. J. Loskutoff for human U-937 cells. Supported by NIH grant GM23562 and by a grant from the National Foundation for Cancer Research. K.W. was supported by a JSPS (Japan Society for the Promotion of Science) Postdoctoral Fellowships for Research Abroad.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.