Culture conditions required to induce capacitation and the acrosome reaction of canine sperm in vitro

Veterinary Record

Volumn 144, Issue 1, 1999, Pages 22-23

  Hewitt, D A ^a   England, G C W ^a  

^a ROYAL VETERINARY COLLEGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACROSOME REACTION; ANIMAL; ARTICLE; CELL SURVIVAL; CULTURE MEDIUM; CULTURE TECHNIQUE; DOG; MALE; METHODOLOGY; PHYSIOLOGY; SPERMATOZOON CAPACITATION; TEMPERATURE;

ACROSOME REACTION; ANIMALS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CULTURE MEDIA; DOGS; MALE; SPERM CAPACITATION; TEMPERATURE;

EID: 0033513618     PISSN: 00424900     EISSN: None     Source Type: Journal    
DOI: 10.1136/vr.144.1.22     Document Type: Article

References (5)

1. BAVISTER, B. D., LIEBFRIED, M. L. & LIEBERMAN, G. (1983) Development of pre-implantation embryos of the golden hamster in a defined culture medium. Biology of Reproduction 28, 235-247
2. BAVISTER, B. D. & YANAGIMACHI, R. (1977) The effects of sperm extracts and energy sources on the motility and acrosome reaction of hamster spermatozoa in vitro. Biology of Reproduction 16, 228-237
3. HEWITT, D. A. & ENGLAND, G. C. W. (1998) An investigation of capacitation and the acrosome reaction in dog spermatozoa using a dual fluorescent staining technique. Animal Reproduction Science 51, 321-332
4. MAHI, C. A. & YANAGIMACHI, R. (1978) Capacitation, acrosome reaction and egg penetration by canine spermatozoa in a simple defined medium. Gamete Research 1, 101-109
5. WARD, C. R. & STOREY, B. T. (1984) Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay. Developmental Biology 104, 287-296