Effects of serum deprivation, insulin and dexamethasone on polysome percentages in C2C12 myoblasts and differentiating myoblasts

Tissue and Cell

Volumn 31, Issue 4, 1999, Pages 451-458

  Smith, C W ^a   Klaasmeyer, J G ^b   Edeal, J B ^b   Woods, T L ^c   Jones, S J ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b University of Nebraska Lincoln Agricultural Research Division   (United States)

^c UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Differentiation; Initiation; Myoblasts; Ribosomes; Translation

Indexed keywords

DEXAMETHASONE; INSULIN; MESSENGER RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; MOUSE; MUSCLE DEVELOPMENT; MYOBLAST; NONHUMAN; POLYSOME; PROTEIN SYNTHESIS; RIBOSOME; RNA TRANSLATION; SERUM RESPONSIVE ELEMENT;

ANIMALIA;

EID: 0033512552     PISSN: 00408166     EISSN: None     Source Type: Journal    
DOI: 10.1054/tice.1999.0053     Document Type: Article

References (37)

1. Anderson A. K. Insulin-stimulated protein synthesis in submandibular acinar cells: interaction with adrenergic and cholinergic agonists. Horm. Metab. Res., 20:1988;20-23.
2. Ashford A. J., Pain V. M. Effect of diabetes on the rate of synthesis and degradation of ribosomes in rat muscle and liver in vivo. J. Biol. Chem., 261:1986;4059-4065.
3. Bains W., Ponte P., Blau H., Kedes L. Cardiac actin is the major gene product in skeletal muscle cell differentiation in vitro. Mol. Cell. Biol., 4:1984;1449-1453.
4. Blackshear P. J. Insulin-stimulated protein biosynthesis as a paradigm of protein kinase C-independent growth factor action. Clin. Res., 37:1989;15-25.
5. Blau H. M., Pavlath G. K., Hardeman E. C. Plasticity of the differentiated state. Science, 230:1985;758-766.
6. Brooks S. P. J. A program for analyzing enzyme rate data obtained from a micro plate reader. Biotechniques, 17:1994;1154-1161.
7. Davies E., Abe S. Methods for isolation and analysis of polyribosomes. Methods in Cell Biology, 50:1995;209-222.
8. Davies E., Dumont J. E., Vassart G. Improved techniques for the isolation of intact thyroglobulin-synthesizing polysomes. Anal. Biochem., 80:1977;289-297.
9. Desler M. M., Jones S. J., Smith C. W., Woods T. L. Effects of dexamethasone and anabolic agents on proliferation and protein synthesis and degradation in C2C12 myogenic cells. J. Anim. Sci., 74:1996;1265-1273.
10. Doestchman T. C., Dym H., Siegel E. J., Heywood S. M. Myoblast stored myosin heavy chain transcripts are precursors to the myotube polysomal myosin heavy chain mRNAs. Diffentiation, 16:1980;149-162.
11. Duncan R., McConkey E. H. Rapid alterations in initiation rate and recruitment of inactive RNA. Eur J Biochem., 128:1982;539-544.
12. Florini J. R. Assay of creatine kinase in microtiter plates using thio-NAD to allow monitoring at 405 nm. Ana. Biochem., 182:1989;399-404.
13. Freshney R. I. Culture of Animal Cells; A Manual of Basic Technique, 2nd edn. Alan R. Liss:1987;New York.
14. Hansson M. L., Ingelman-Sundberg M. Insulin stimulated protein synthesis in adipocytes. Enhanced rate of initiation associated with increased phosphorylation of ribosomal protein S6. Eur. J. Biochem., 115:1985;97-100.
15. Harmon C. S., Proud C. G., Pain V. M. Effects of starvation, diabetes and acute insulin treatment on the regulation of polypeptide-chain initiation in rat skeletal muscle. Bichem., 223:1984;687-696.
16. Hershey J. W. B. Translational control in mammalian cells. Annu. Rev. Biochem., 60:1991;717-755.
17. Hesketh J. E., Campbell G. P. Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism. Bioscience Reports, 7:1987;533-541.
18. Johanneson A., Pryme I. F., Vedeler A. Changes in distribution of actin mRNA in different polysome fractions following stimulation of MPC-11 cells. Mol. Cell Biochem., 142:1995;107-115.
19. Labarca C., Paigen K. A rapid and sensitive method DNA assay procedure. Anal. Biochem., 102:1980;344-352.
20. Larsen D. E., Zahradka P., Sells B. H. Control points in eukaryotic ribosome biogenesis. Biochem. Cell Biol., 69:1991;5-22.
21. Miller D. S. Stimulation of protein synthesis in stage IV Xenopus oocytes by microinjected insulin. J. Biol. Chem., 264:1989;10438-10446.
22. Montine K. S., Henshaw E. C. Serum growth factors cause rapid stimulation of protein synthesis and dephosphorylation of eIF-2 in serum deprived Ehrlich cells. Biochem. Biophys. Acta., 101:1989;282-288.
23. Munro, H. N. Fleck, A. 1969.
24. Nielsen P. J., Duncan R., McConkey E. H. Phosphorylation of ribosomal protein S6. Relationship to protein synthesis in HeLa cells. Euro. J. Biochem., 120:1981;523-527.
25. Okabayashi Y., Moessner J., Logsdon C. D., Goldfine I. D., Williams J. A. Insulin and others stimulants have nonparallel translational effects on protein synthesis. Diabetes, 36:1987;1054-1060.
26. Pain V. M. Translational control during amino acid starvation. Biochimie, 76:1994;718-728.
27. Palmer R. M., Thompson M. G., Knott R. M., Campbell G. P., Thom A., Morrison K. S. Insulin and insulin-like growth factor-I responsiveness and signaling mechanisms in C2C12 satellite cells: effects of differentiation and fusion. Biochim. Biophys. Acta., 1355:1997;167-176.
28. SAS, 1991, SAS/STAT User's Guide, SAS Institute Inc. NC.
29. Steele R. G. D., Torrie J. H. Principles and Procedures of Statistics, 2nd edn. 1980;. p. New York.
30. Thomas G., Martin-Perez J., Siegman M. The effect of serum EGF, PGF2 alpha and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis. Cell, 30:1982;235-242.
31. Vandenburg C. R., Nathanson M. A. Postranscriptional control of embryonic skeletal muscle protein synthesis. Control at the level of translation by endogenous RNA. J. Cell Biol., 107:1988;1085-1098.
32. van Venrooij W. J. W., Henshaw E. C., Hirsch C. A. Effects of deprival of glucose or individual amino acid on polyribosome distribution and rate of protein synthesis in cultured mammalian cell. Biochem Biopys. Acta, 239:1972;127-137.
33. van Venrooij W. J. W., Sillekens P. T. G., van Eekelen C. A. G., Reinders T. T. On the association with the cytoskeleton of uninfected and andenovirus-infected human KB cells. Exp. Cell Res., 135:1981;79-91.
34. Wernerman J., von der Decken A., Vinnars E. Polyrobosome concentration in human skeletal muscle after starvation and parenteral or enteral refeeding. Metab. Clin. Exp., 35:1986a;447-451.
35. Wernerman J., von der Decken A., Vinnars E. The interpretation of ribosome determinations to assess protein synthesis in human skeletal muscle. Infusionsthereaoie Klinische Ernahrung, 13:1986b;162-165.
36. White M. E., Allen C. E., Dayton W. R. Effect of sera from fed and fasted pigs on proliferation and protein turnover in culture myogenic cells. J. Anim. Sci., 66:1988;34-40.
37. Zahradka P., Larson D. E. Post-translational regulation of 5S rRNA synthesis following myoblast differentiation. Eur. J. Biochem., 184:1989;261-266.