Direct binding and in vivo regulation of the fission yeast p21-activated kinase Shk1 by the SH3 domain protein Scd2

Molecular and Cellular Biology

Volumn 19, Issue 12, 1999, Pages 8066-8074

  Chang, Eric ^a   Bartholomeusz, Geoffrey ^b   Pimental, Ruth ^b   Chen, Jing ^a   Lai, Hong ^b   Wang, Li Hua L ^a   Yang, Peirong ^b   Marcus, Stevan ^b  

^a NEW YORK UNIVERSITY   (United States)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P21;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; ENZYME ACTIVATION; ENZYME BINDING; ENZYME REGULATION; IN VIVO STUDY; NONHUMAN; ONCOGENE RAS; PRIORITY JOURNAL; PROTEIN DOMAIN; SCHIZOSACCHAROMYCES POMBE; YEAST;

FUNGI; SCHIZOSACCHAROMYCES POMBE;

EID: 0033500151     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.19.12.8066     Document Type: Article

References (44)

1. Abo, A., J. Qu, M. S. Cammarano, C. Dan, A. Fritsch, V. Baud, B. Belisle, and A. Minden. 1998. PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia. EMBO J. 17:6527-6540.
2. Alfa, C., P. Fantes, J. Hyams, M. McLeod, and E. Warbrick, 1993. Experiments with fission yeast: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
3. Bagrodia, S., S. J. Taylor, K. A. Jordon, L. Van Aelst, and R. A. Cerione. 1998. A novel regulator of p21-activated kinases. J. Biol. Chem. 273:23633-23636.
4. Bokoch, G. M., V. Wang, B. P. Bohl, M. A. Sells, L. A. Quilliam, and U. G. Knaus. 1996. Interaction of the Nck adapter protein with p21-activated kinase (Pak1). J. Biol. Chem. 271:25746-25749.
5. Brzeska, H., R. Young, U. Knaus, and E. D. Korn. 1999. Myosin I heavy chain kinase: cloning of the full-length gene and acidic lipid-dependent activation by Rac and Cdc42. Proc. Natl. Acad. Sci. USA 96:394-399.
6. Burbelo, P. D., D. Drechsel, and A. Hall. 1995. A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases. J. Biol. Chem. 270:29071-29074.
7. Chang, E. C., M. Barr, Y. Wang, V. Jung, H. P. Xu, and M. H. Wigler. 1994. Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Cell 79:131-141.
8. Chen, C. R., Y. C. Li, J. Chen, M. C. Hou, P. Papadaki, and E. C. Chang. 1999. Moel, a conserved protein in Schizosaccharomyces pombe, interacts with a Ras effector, Scd1, to affect proper spindle formation. Proc. Natl. Acad. Sci. USA 96:517-522.
9. Cvrckova, F., C. De Virgilio, E. Manser, J. R. Pringle, and K. Nasmyth. 1995. Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev. 9:1817-1830.
10. Daniels, R. H., F. T. Zenke, and G. M. Bokoch. 1999. alpha-Pix stimulates p21-activated kinase activity through exchange factor-dependent and -independent mechanisms. J. Biol. Chem. 274:6047-6050.
11. Faure, S., S. Vigneron, M. Doree, and N. Morin. 1997. A member of the Ste20/PAK family of protein kinases is involved in both arrest of Xenopus oocytes at G2/prophase of the first meiotic cell cycle and in prevention of apoptosis. EMBO J. 16:5550-5561.
12. Faure, S., S. Vigneron, S. Galas, T. Brassac, C. Delsert, and N. Morin. 1999. Control of G2/M transition in Xenopus by a member of the p21-activated kinase (PAK) family: a link between protein kinase A and PAK signaling pathways? J. Biol. Chem. 274:3573-3579.
13. Frost, J. A., A. Khokhlatchev, S. Stippec, M. A. While, and M. H. Cobb. 1998. Differential effects of PAK1-activating mutations reveal activity-dependent and -independent effects on cytoskeletal regulation. J. Biol. Chem. 273:28191-28198.
14. Frost, J. A., S. Xu, M. R. Hutchison, S. Marcus, and M. H. Cobb. 1996. Actions of Rho family small G proteins and p21-activated protein kinases on mitogen-activated protein kinase family members. Mol. Cell. Biol. 16:3707-3713.
15. Fukui, Y., and M. Yamamoto. 1988. Isolation and characterization of Schizosaccharomyces pombe mutants phenotypically similar to ras1. Mol. Gen. Genet. 215:26-31.
16. Gilbreth, M., P. Yang, G. Bartholomeusz, R. A. Pimental, S. Kansra, R. Gadiraju, and S. Marcus. 1998. Negative regulation of mitosis in fission yeast by the Shk1-interacting protein Skb1 and its human homolog, Skb1Hs. Proc. Natl. Acad. Sci. USA 95:14781-14786.
17. Gilbreth, M., P. Yang, D. Wang, J. Frost, A. Polverino, M. H. Cobb, and S. Marcus. 1996. The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog. Proc. Natl. Acad. Sci. USA 93:13802-13807.
18. Hughes, D. A., A. Ashworth, and C. J. Marshall. 1993. Complementation of byr1 in fission yeast by mammalian MAP kinase kinase requires coexpression of Raf kinase. Nature 364:349-352.
19. Leberer, E., D. Dignard, D. Harcus, D. Y. Thomas, and M. Whiteway. 1992. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J. 11:4815-4824.
20. Leeuw, T., A. Fourest-Lieuvin, C. Wu, J. Chenevert, K. Clark, M. Whiteway, D. Y. Thomas, and E. Leberer. 1995. Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin. Science 270:1210-1213.
21. Manser, E., H. Y. Huang, T. H. Loo, X. Q. Chen, J. M. Dong, T. Leung, and L. Lim. 1997. Expression of constitutively active α-Pak reveals effects of the kinase on actin and focal complexes. Mol. Cell. Biol. 17:1129-1143.
22. Manser, E., T. Leung, H. Salihuddin, Z. S. Zhao, and L. Lim. 1994. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 367:40-46.
23. Manser, E., T. H. Loo, C. G. Koh, Z. S. Zhao, X. Q. Chen, L. Tan, I. Tan, T. Leung, and L. Lim. 1998. Pak kinases are directly coupled to the Pix family of nucleotide exchange factors. Mol. Cell 1:183-192.
24. PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 92:6180-6184.
25. Maundrell, K. 1990. nmtI of fission yeast. A highly transcribed gene completely repressed by thiamine. J. Biol. Chem. 265:10857-10864.
26. Miller, P. J., and D. I. Johnson. 1994. Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe. Mol. Cell. Biol. 14:1075-1083.
27. Ottilie, S., P. J. Miller, D. I. Johnson, C. L. Creasy, M. A. Sells, S. Bagrodia, S. L. Forsburg, and J. Chernoff. 1995. Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating. EMBO J. 14:5908-5919.
28. Pimental, R., and S. Marcus. Unpublished results.
29. Polverino, A., J. Frost, P. Yang, M. Hutchison, A. M. Neiman, M. H. Cobb, and S. Marcus. 1995. Activation of mitogen-activated protein kinase cascades by p21-activated protein kinases in cell-free extracts of Xenopus oocytes. J. Biol. Chem. 270:26067-26070.
30. Ramer, S. W., and R. W. Davis. 1993. A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90:452-456.
31. Rose, M. D., F. Winston, and P. Hieter. 1990. Methods in yeast genetics: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
32. Rudel, T., and G. M. Bokoch. 1997. Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2. Science 276:1571-1578.
33. Sawin, K. E., and P. Nurse. 1998. Regulation of cell polarity by microtubules in fission yeast. J. Cell Biol. 142:457-471.
34. Sells, M. A., J. T. Barratt, J. Caviston, S. Ottilie, E. Leberer, and J. Chernoff. 1998. Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast. J. Biol. Chem. 273:18490-18498.
35. Sells, M. A., and J. Chernoff. 1997. Emerging from the Pak - the p21-activated protein kinase family. Trends Cell Biol. 7:162-167.
36. Sells, M. A., U. G. Knaus, S. Bagrodia, D. M. Ambrose, G. M. Bukoch, and J. Chernoff. 1997. Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Curr. Biol. 7:202-210.
37. Shibuya, E. K., A. J. Polverino, E. Chang, M. Wigler, and J. V. Ruderman. 1992. Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes. Proc. Natl. Acad. Sci. USA 89:9831-9835.
38. Tu, H., and M. Wigler. 1999. Genetic evidence for Pak1 autoinhibition and its release by Cdc42. Mol. Cell. Biol. 19:602-611.
39. Van Aelst, L., M. Barr, S. Marcus, A. Polverino, and M. Wigler. 1993. Complex formation between RAS and RAF and other protein kinases. Proc. Natl. Acad. Sci. USA 90:6213-6217.
40. Verde, F., D. J. Wiley, and P. Nurse. 1998. Fission yeast Orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle. Proc. Natl. Acad. Sci. USA 95:7526-7531.
41. Vojtek, A. B., S. M. Hollenberg, and J. A. Cooper. 1993. Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 74:205-214.
42. Wang, Y., H. P. Xu, M. Riggs, L. Rodgers, and M. Wigler. 1991. byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype. Mol. Cell. Biol. 11:3554-3563.
43. Yang, P., S. Kansra, R. A. Pimental, M. Gilbreth, and S. Marcus. 1998. Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast. J. Biol. Chem. 273:18481-18489.
44. Zhang, S., J. Han, M. A. Sells, J. Chernoff, U. G. Knaus, R. J. Ulevitch, and G. M. Bokoch. 1995. Rho family GTPases regulate p38 mitogen-activated protein kinase through the downstream mediator Pak1. J. Biol. Chem. 270: 23934-23936.