Genetic and biochemical characterization of an exopolygalacturonase and a pectate lyase from Yersinia enterocolitica

Canadian Journal of Microbiology

Volumn 45, Issue 5, 1999, Pages 396-403

  Liao, Ching Hsing ^a   Revear, Larry ^a   Hotchkiss, Arland ^a   Savary, Brett ^a  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

HPLC analysis; Human pathogen; Pectinase activities; pehY gene

Indexed keywords

PECTATE LYASE; POLYGALACTURONASE; RECOMBINANT ENZYME;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ERWINIA; ESCHERICHIA COLI; GENE ISOLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RALSTONIA SOLANACEARUM; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; YERSINIA ENTEROCOLITICA;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CHRYSANTHEMI; ESCHERICHIA COLI; NEGIBACTERIA; RALSTONIA SOLANACEARUM; YERSINIA ENTEROCOLITICA;

EID: 0033492928     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w99-034     Document Type: Article

References (29)

1. Badger, J.L., and Miller, V.L. 1995. Role of RpoS in survival of Yersinia enterocolitica to a variety of environmental stresses. J. Bacteriol. 177: 5370-5373.
2. Bagley, S.T., and Starr, M.P. 1979. Characterization of intracellular polygalacturonic acid trans-eliminase from Klebsiella oxytoca, Yersinia enterocolitica, and Erwinia chrysanthemi. Curr. Microbiol. 2: 381-386.
3. Beuchat, L.R. 1996. Pathogenic microorganisms associated with fresh produce. J. Food Prot. 59: 204-216.
4. Catteau, M., Krembel, C., and Wauters, G. 1985. Yersinia enterocolitica in raw vegetables. Sci. Aliments, 5: 103-106.
5. Chatterjee, A.K., Buchanan, G.E., Behrens, M.K., and Starr, M.P. 1979. Synthesis and excretion of polygalacturonic acid trans-eliminase in Erwinia, Yersinia, and Klebsiella species. Can. J. Microbiol. 25: 94-102.
6. Collmer, A., Ried, J.L., and Mount, M.S. 1988. Assay methods for pectic enzymes. Methods Enzymol. 161: 329-335.
7. Cover, T.L., and Aber, R.C. 1989. Yersinia enterocolitica. N. Engl. J. Med. 321: 16-24.
8. Darbas, H., Riviere, M., and Obertic, J. 1985. Yersinia in refrigerated vegetables. Sci. Aliments 5: 81-84.
9. He, S.Y., and Collmer, A. 1990. Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-α-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16. J. Bacteriol. 172: 4988-4995.
10. Herlache, T.C., Hotchkiss, A.T. Jr., Burr, T.J., and Collmer, A. 1997. Characterization of the Agrobacterium vitis pehA gene and comparison of the encoded polygalacturonase with the homologous enzymes from Erwinia carotovora and Ralstonia solanacearum. Appl. Environ. Microbiol. 63: 338-346.
11. Hotchkiss, A.T., and Hicks, K.B. 1990. Analysis of oligogalacturonic acids with 50 or fewer residues by high-performance anion-exchange chromatography and pulsed amperometric detection. Anal. Biochem. 184: 200-206.
12. Huang, Q., and Allen, C. 1997. An exo-poly-α-galacturonosidase, pehB, is required for wild-type virulence of Ralstonia solanacearum. J. Bacteriol. 179: 7369-7378.
13. Karapinar, M., and Gonul, S.A. 1992. Removal of Yersinia enterocolitica from fresh parsley by washing with acetic acid and vinegar. Intl. J. Food Microbiol. 16: 261-264.
14. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227: 680-685.
15. Liao, C.-H. 1989. Analysis of pectate lyase produced by soft rot bacteria associated with spoilage of vegetables. Appl. Environ. Microbiol. 55: 1677-1683.
16. Liao, C.-H., and Wells, J.M. 1987. Diversity of pectolytic, fluorescent pseudomonads causing soft rots of fresh vegetables at produce markets. Phytopathology, 77: 673-677.
17. Liao, C.-H., Gaffney, T.D., Bradley, S.P., and Wong, L.J.C. 1996. Cloning of a pectate lyase gene from Xanthomonas campestris pv. malvacearum and comparison of its sequence relationship with pel genes of soft-rot Erwinia and Pseudomonas. Mol. Plant-Microbe Interact. 9: 14-21.
18. Manulis, S., Kobayashi, D.Y., and Keen, N.T. 1988. Molecular cloning and sequencing of a pectate lyase gene from Yersinia pseudotuberculosis. J. Bacteriol. 170: 1825-1830.
19. Nakajima, H., Inoue, M., Mori, T., Itoh, K.-I., Arakawa, E., and Watanabe, H. 1992. Detection and identification of Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica by an improved polymerase chain reaction method. J. Clin. Microbiol. 30: 2484-2486.
20. Nguyen-the, C., and Carlin, F. 1994. The microbiology of minimally processed fresh fruits and vegetables. Crit. Rev. Food Sci. Nutr. 34: 371-401.
21. Preston, J.F., Rice, D., Ingram, L.O., and Keen, N.T. 1992. Differential depolymerization mechamism of pectate lyase secreted by Erwinia chrysanthemi EC16. J. Bacteriol. 174: 2039-2042.
22. Robins-Browne, R.M. 1997. Yersinia enterocolitica. In Food microbiology: fundamentals and frontiers. Edited by M.P. Doyle, L.R. Beuchat, and T.J. Montville. American Society for Microbiology, Washington, D.C. pp. 192-215.
23. Ruvun, G.B., and Ausubel, F.M. 1981. A general method for site-directed mutagenesis in prokaryotes. Nature (London), 289: 85-88.
24. Sambrook, J., Fritsch, E.F., and Maniatis, T.A. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Starr, M.P., Chatterjee, A.K., Starr, P.B., and Buchanan, G.E. 1977. Enzymatic degradation of polygalacturonic acid by Yersinia and Klebsiella species in relation to clinical laboratory procedures. J. Clin. Microbiol. 6: 379-386.
26. Staskawicz, B., Dahlbeck, D., Keen, N., and Napoli, C. 1987. Molecular characterization of cloned avirulence gene from race 0 to race 1 of Pseudomonas sringae pv. glycinea. J. Bacteriol. 169: 5789-5794.
27. Tacket, C.O., Ballard, J., Harris, N., Allard, J., Nolan, C., Quan, T., and Cohen, M.L. 1985. An outbreak of Yersinia enterocolitica infection caused by contaminated tofu (soybean curd). Am. J. Epidemiol. 121: 705-711.
28. Von Riesen, V.L. 1975. Polypectate digestion by Yersinia. J. Clin. Microbiol. 2: 552-553.
29. Witholt, B., Boekhout, M., Brock, M., Kingma, J., Van Heerikhuizen, H., and DeLeij, L. 1976. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal. Biochem. 74: 160-170.