A new methodology for plant cell viability assessment using intracellular esterase activity

Plant Cell Reports

Volumn 19, Issue 2, 1999, Pages 171-176

  Steward, N ^a,b   Martin, R ^c   Engasser, J M ^a   Goergen, J L ^a,b  

^a CNRS   (France)

^b L ORÉAL RECHERCHE   (France)

^c NONE

Author keywords

Cytoplasmic esterase activity; Plant cell culture; Viability

Indexed keywords

BATCH PROCESS; CELL MEMBRANE; CELL METABOLISM; CELL VIABILITY; ENZYME ACTIVITY; ESTERASE; PLANT CELL CULTURE; SUSPENSION CELL CULTURE;

MEDICAGO SATIVA;

EID: 0033492697     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990050729     Document Type: Article

References (35)

1. Akatov VS, Lezhnev EI, Vexier AM, Kublik LN (1985) Low pH value of pericellular medium as a factor limiting cell proliferation in dense cultures. Exp Cell Res 160:412-418
2. Altman SA, Randers L, Rao G (1993) Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. Biotechnol Prog 9:671-674
3. Archambault J (1991) Large-scale (20 l) culture of surface-immobilized Catharanthus roseus cells. Enzyme Microbiol Technol 13:882-892
4. Brown JT, Hegarty PK, Charlwood BV (1987) The toxicity of monoterpenes to plant cell cultures. Plant Sci 48:195-201
5. Bursch W, Kleine L, Tenniswood M (1990) The biochemistry of cell death by apoptosis. Biochem Cell Biol 68:1071-1074
6. Chaubet N, Pétiard V, Pareilleux A (1981) β-galactosidases of suspension-cultured Medicago sativa cells growing on lactose Plant Sci Lett 22:369-378
7. Cook JA, Mitchell JB (1989) Viability measurements in mammalian cell systems. Anal Biochem 179:1-7
8. Franek F, Dolnikova J (1991) Hybridoma growth and monoclonal antibody production in iron rich protein-free medium: effect of nutrient concentration. Cytotechnology 7:33-38
9. Goergen JL, Marc A, Engasser JM (1993) Determination of cell lysis and death kinetics in continuous hybridoma cultures from the measurement of lactate deshydrogenase release Cytotechnology 11:189-195
10. Guardiola J, Iborra JL, Canovas M (1994) A model that links growth and secondary metabolite production in plant cell suspension cultures. Biotechnol Bioeng 46:291-297
11. Heller R (1953) Researches on the mineral nutrition of plant tissues. Ann Sci Nat Biol Veg 14:1-223
12. Hergaty PK, Smart NJ, Scragg AH, Fowler MW (1986) The aeration of Catharanthus roseus L.G. Don suspension cultures in airlift bioreactors: the inhibitory effect at high aeration rates on culture growth. J Exp Bot 37:1911-1920
13. Hooker BS, Lee JM, An G (1989) Response of plant tissue culture to a high shear environment. Enzyme Microb Technol 11:484-490
14. Hou BH, Lin CG (1996) Rapid optimization of electroporation conditions for soybean and tomato suspension cultured cells Plant Physiol 111 [Suppl 2]: 166
15. Huang CN, Cornejo MJ, Bush DS, Jones RL (1986) Estimating viability of plant protoplasts using double and single staining Protoplasma 135:80-87
16. Ishikawa M, Robertson AJ, Gusta LV (1995) Comparison of viability tests for assessing cross-adaptation to freezing, heat and salt stresses induced by abscisic acid in bromegrass (Bromus inermis Leyss) suspension cultured cells. Plant Sci 107:83-93
17. Laloue M, Courtois D, Manigault P (1980) Convenient and rapid fluorescent staining of plant cell nuclei with '33258' Hoechst. Plant Sci Lett 17:175-179
18. Leborgne N, Teulieres C, Travert S, Rols MP, Teissie J, Boudet AM (1995) Introduction of specific carbohydrates into Eucalyptus gunnii cells increases their freezing tolerance. Eur J Biochem 229:710-717
19. Matanguihan RM, Konstantinov KB, Yoshida T (1994) Dielectric measurement to monitor the growth and the physiological states of biological cells. Bioprocess Eng 11:213-222
20. Morel G, Wetmore RH (1951) Fern callus tissue culture. Am J Bot 38:141-143
21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-496
22. Nash DT, Davies ME (1975) Isoenzyme changes during the growth cycle of Paul's scarlet rose cell suspensions. Phytochemistry 14:2113-2118
23. Prenosil JE, Hegglin M (1991) Self-immobilized plant cell aggregates in a bioreactor system with low shear stress. Ann NY Acad Sci 613:234-247
24. Reish B, Duke SH, Bingham ET (1981) Selection and caracterisation of ethionine-resistant alfalfa (Medicago sativa L.) cell lines. Theor Appl Genet 59:89-94
25. Smith BA, Reider ML, Fletcher JS (1982) Relationship between vital staining and subculture growth during the senescence of plant tissue culture. Plant Physiol 70:1228-1230
26. Steponkus PL, Lanphear FO (1967) Refinment of the triphenyltetrazolium chloride method of determining cold injury. Plant Physiol 42:1423-1426
27. Su WW (1995) Bioprocessing technology for plant cell suspension culture. Appl Biochem Biotechnol 50:189-230
28. Swain D, De DN (1994) Vital staining, a technique for rapid screening of plant protoplast viability. Indian J Exp Biol 32:501-506
29. Takeda T, Seki M, Furusaki S (1994) Hydrodynamic damage of cultured cells of Carthamus tinctorius in a stirred tank reactor J Chem Eng Jpn 27:466-471
30. Thomas JE, Roberts RS (1989) Effect of hyperosmotic pressure on growth of L5178 Y cells. Process Biochem 24:179-182
31. Towill LE, Mazur P (1974) Studies on the reduction of 2,3,5-triphenyltretrazolium chloride as a viability assay for plant tissue cultures. Can J Bot 53:1097-1102
32. Wagner A, Marc A, Engasser JM (1992) The use of lactate dehydrogenase (LDH) release kinetics for the evaluation of death and growth of mammalian cells in perfusion bioreactors. Biotechnol Bioeng 39:320-326
33. Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells Stain Technol 47:189-194
34. Yuhas JM, Toya RE, Pazmino NH (1974) Neuraminidase and cell viability: failure to detect cytotoxic effects with dye-exclusion techniques. J Natl Cancer Inst 53:465-468
35. Zhong JJ, Fujiyama K, Seki T, Yoshida T (1994) A quantitative analysis of shear effects on cell suspension and cell culture of Perilla frutescens in bioreactors. Biotechnol Bioeng 44:649-654