Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues

Plant Cell Reports

Volumn 19, Issue 2, 1999, Pages 118-127

  Rasco Gaunt, S ^a   Riley, A ^a   Barcelo, P ^a   Lazzeri, P A ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

DNA delivery; Particle bombardment; Transient GUS expression; Triticum aestivum

Indexed keywords

AUXIN; BIOLISTIC TRANSFORMATION; CALCIUM CHLORIDE; CALCIUM ION; DNA TRANSFER; GENE EXPRESSION; GOLD; PARTICLE; PARTICLE SIZE; PLASMID DNA; SPERMIDINE; TISSUE CULTURE; TRANSIENT TRANSFECTION; VACUUM;

TRITICUM AESTIVUM;

EID: 0033491734     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990050721     Document Type: Article

References (23)

1. Altpeter F, Vasil V, Srivastava V, Stoger E, Vasil IK (1996) Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep 16:12-17
2. Barcelo P, Lazzeri PA (1995) Transformation of cereals by microprojectile bombardment of immature inflorescence and scutellum tissues. In: Jones H (ed) Methods in molecular biology: plant gene transfer and expression protocols. Humana Press, Totowa N.J., pp 113-123
3. Barro F, Rook L, Bekes F, Gras P, Tatham AS, Fido R, Lazzeri PA, Shewry PR, Barcelo P (1997) Transformation of wheat with high molecular weight subunit genes results in improved functional properties. Nat Biotechnol 15:1295-1299
4. Becker D, Brettschneider R, Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue Plant J 5:299-307
5. Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5:213-218
6. Hunold R, Bronner R, Hahne G (1994) Early events in microprojectile bombardment: cell viability and particle location. Plant J 5:593-604
7. Iglesias VA, Gisel A, Bilang R, Leduc N, Potrykus I, Sautter C (1994) Transient expression of visible marker genes in meristem cells of wheat embryos after ballistic micro-targetting. Planta 192:84-91
8. Jain RK, Jain S, Wang B, Wu R (1996) Optimization of biolistic method for transient gene expression and production of agronomically useful transgenic Basmatic rice plants. Plant Cell Rep 15:963-968
9. Klein TM, Gradziel T, Fromm ME, Sanford JC (1988) Factors influencing gene delivery into Zea mays cells by high-velocity microprojectiles. Bio/Technology 6:559-563
10. Lazzeri PA, Shewry PR (1993) Biotechnology of cereals. In: Tombs MP (ed) Biotechnology and genetic engineering reviews, vol II. Intercept, Andover, Md., pp 79-145
11. Loeb TA, Reynolds TL (1994) Transient expression of the uidA gene in pollen embryoids of wheat following microprojectile bombardment. Plant Sci 104:81-91
12. Lonsdale D, Ontec S, Cuming A (1990) Transient expression of exogenous DNA in intact, viable wheat embryos following particle bombardment. J Exp Bot 41:1161-1165
13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497
14. Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5:285-297
15. Ortiz JPA, Reggiardo MI, Ravizzini RA, Altabe SG, Cervigni GDL, Spitteler MA, Morata MM, Elias FE, Vallejos RH (1996) Hygromycin resistance as an efficient selectable marker for wheat stable transformation. Plant Cell Rep 15:877-881
16. Perl A, Kless H, Blumenthal A, Galili G, Galunj E (1992) Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization. Mol Gen Genet 235:279-284
17. Rasco-Gaunt S, Barcelo P (1999) Immature inflorescence culture of cereals: a highly responsive system for regeneration and transformation. In: RD Hall (ed) Plant cell culture protocols. (Methods in molecular biology series). Humana Press, Totowa N.J. 71-81
18. Ratnayaka IJS, Oard JH (1995) A rapid method to monitor DNA precipitation onto microcarriers before particle bombardment Plant Cell Rep 14:794-798
19. Taylor MG, Vasil IK (1991) Histology of and physical factors affecting transient GUS expression in pearl millet (Pennisetum glaucum (L.) R. Br.) embryos following microprojectile bombardment. Plant Cell Rep 10:120-125
20. Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus Bio/ Technology 10:667-674
21. Weeks T, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077-1084
22. Yamashita T, Iida A, Morikawa H (1991) Evidence that more than 90% of β-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus. Plant Physiol 97:829-831
23. Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylor ML, Rodriguez D, Pajeau ME, Brown SM, Santino CG, JE Fry (1995) Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Rep 15:159-163