Heterologous expression, purification, and kinetic comparison of the cytoplasmic and mitochondrial glyoxalase II enzymes, GLo2p and GLo4p, from Saccharomyces cerevisiae

Protein Expression and Purification

Volumn 17, Issue 3, 1999, Pages 456-464

  Bito, Arnold ^a   Haider, Margit ^a   Briza, Peter ^a   Strasser, Peter ^b   Breitenbach, Michael ^a  

^a UNIVERSITY OF SALZBURG   (Austria)

^b ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; ARABIDOPSIS; CYTOPLASM; ENZYME PURIFICATION; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE PRODUCT; HETEROLOGOUS PRODUCT; HYDROXYACYLGLUTATHIONE HYDROLASE; MASS SPECTROMETRY; MITOCHONDRION; MOLECULAR WEIGHT; PH; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; RECOMBINANT PROTEIN; S LACTOYLGLUTATHIONE; SACCHAROMYCES CEREVISIAE;

EID: 0033486132     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1151     Document Type: Article

References (27)

1. Thornalley P. J. The glyoxalase system: New developments towards functional characterization of a metabolic pathway fundamental to biological life. Biochem. J. 269:1990;1-11.
2. Richard J. P. Mechanism for the formation of methylglyoxal from triosephosphates. Biochem. Soc. Trans. 21:1993;549-553.
3. Mannervik B., Ridderström M. Catalytic and molecular properties of glyoxalase I. Biochem. Soc. Trans. 21:1993;515-517.
4. Vander Jagt D. L. Glyoxalase II: Molecular characteristics, kinetics and mechanism. Biochem. Soc. Trans. 21:1993;522-527.
5. Creighton D. L., Migliorini M., Pourmotabbed T., Guha M. K. Optimization of efficiency in the glyoxalase pathway. Biochemistry. 27:1988;7376-7384.
6. Ferguson G. P., Tötemeyer S., MacLean M. J., Booth I. R. Methylglyoxal production in bacteria: Suicide or survival? Arch. Microbiol. 170:1998;209-219.
7. Thornalley P. J. Modification of the glyoxalase system in disease processes and prospects for therapeutic strategies. Biochem. Soc. Trans. 21:1993;531-534.
8. Crowder M. W., Maiti M. K., Banovic L., Makaroff C. A. Glyoxalase II from A. thaliana requires Zn(II) for catalytic activity. FEBS Lett. 418:1997;351-354.
9. Uotila L. Purification and characterization of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from human liver. Biochemistry. 12:1973;3944-3951.
10. Ball J. C., Vander Jagt D. L. Purification of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from rat erythrocytes. Anal. Biochem. 98:1979;472-477.
11. Oray B., Norton S. J. Glyoxalase II from mouse liver. Methods Enzymol. 90:1982;547-551.
12. Allen R. E., Lo T. W. C., Thornalley P. J. Purification and characterization of glyoxalase II from human red blood cells. Eur. J. Biochem. 213:1993;1261-1267.
13. Norton S. J., Principato G. B., Talesa V., Lupattelli M., Rosi G. Glyoxalase II from Zea mays: Properties and inhibition study of the enzyme purified by use of a new affinity ligand. Enzyme. 42:1989;189-196.
14. Murata K., Inoue Y., Watanabe K., Fukuda Y., Saikusu T., Shimosaka M., Kimura A. purification and characterization of glyoxalase II from Saccharomyces cerevisiae. Agric. Biol. Chem. 50:1986;135-142.
15. Murata K., Sato N., Rhee H.-I., Watanabe K., Kimura A. Purification and characterization of glutathione thiol esterase from Saccharomyces cerevisiae. Agric. Biol. Chem. 51:1987;1901-1907.
16. Ridderström M., Saccucci F., Hellman U., Bergman T., Principato G., Mannervik B. Molecular cloning, heterologous expression, and characterization of human glyoxalase II. J. Biol. Chem. 271:1996;319-323.
17. Ridderström M., Mannervik B. Molecular cloning and characterization of the thiolesterase glyoxalase II from Arabidopsis thaliana. Biochem. J. 322:1997;449-454.
18. Bito A., Haider M., Hadler I., Breitenbach M. Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins. J. Biol. Chem. 272:1997;21509-21519.
19. Principato G. B., Rosi G., Talesa V., Giovannini E., Uotila L. Purification and characterization of two forms of glyoxalase II from the liver and brain of Wistar rats. Biochim. Biophys. Acta. 911:1987;349-355.
20. Talesa V., Uotila L., Koivusalo M., Principato G. B., Giovannini E., Rosi G. Demonstration of glyoxalase II in rat liver mitochondria: Partial purification and occurrence in multiple forms. Biochim. Biophys. Acta. 955:1988;103-110.
21. Uotila L. Thioesters of glutathione. Methods Enzymol. 77:1981;424-430.
22. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
23. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
24. Ellman G. L. Arch. Biochem. Biophys. 82:1959;70-77.
25. Ball J. C., Vander Jagt D. L. S-2-Hydroxyacylglutathione hydrolase (glyoxalase II): Active-site mapping of a nonserine thiolesterase. Biochemistry. 20:1981;899-905.
26. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
27. Inoue Y., Kimura A. Identification of the structural gene for glyoxalase I from Saccharomyces cerevisiae. J. Biol. Chem. 271:1996;25958-25965.