Calreticulin is transported to the surface of NG108-15 cells where it forms surface patches and is partially degraded in an acidic compartment

Journal of Neuroscience Research

Volumn 58, Issue 5, 1999, Pages 652-662

  Xiao, Guangqing ^a   Chung, Tzu Feng ^b   Fine, Richard E ^b,c   Johnson, Robin J ^b  

^a Boston University   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Calcium; Calreticulin; Cell surface; Degradation; KDEL proteins; NG108 15 cell

Indexed keywords

CALCIUM; CALRETICULIN; CELL PROTEIN;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CANCER CELL CULTURE; CELL MEMBRANE; CELL SURFACE; ENDOPLASMIC RETICULUM; HYBRID CELL; IMMUNOCYTOCHEMISTRY; IMMUNOPRECIPITATION; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN TRANSPORT;

ANIMALS; BIOLOGICAL TRANSPORT; BIOTINYLATION; CALCIUM-BINDING PROTEINS; CALRETICULIN; CELL COMPARTMENTATION; CELL MEMBRANE; HYBRID CELLS; IMMUNOHISTOCHEMISTRY; MEMBRANE PROTEINS; MICE; PRECIPITIN TESTS; RATS; RIBONUCLEOPROTEINS; TIME FACTORS;

EID: 0033485892     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19991201)58:5<652::AID-JNR6>3.0.CO;2-H     Document Type: Article

References (50)

1. 2+-binding protein calreticulin and perform, a component of the cytotoxic T-cell granules. Biochem 37:10386-10394.
2. 2+ binding domains. J Biol Chem 266:21458-21465.
3. 2+ stores and reveals aspects of their lumenal microenvironment and function. J Cell Biol 130:847-855.
4. Burns K, Michalak M. 1993. Interactions of calreticulin with proteins of the endoplasmic and sarcoplasmic reticulum membranes. FEBS Lett 318:181-185.
5. Burns K, Duggan B, Atkinson EA, Famulski KS, Nemer M, Bleackley RC, Michalak M. 1994. Modulation of expression by calreticulin binding to the glucocorticoid receptor. Nature 367:476-480.
6. Ciechanover A, Finley D, Varshavsky A. 1984. Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85. Cell 37:57-66.
7. Coppolino MG, Woodside MJ, Demaurex N, Grinstein S, St-Arnaud R, Dedhar S. 1997. Calreticulin is essential for integrin-mediated calcium signaling and cell adhesion. Nature 386:843-847.
8. Daniels MP, Hamprecht B. 1974. The ultrastructure of neuroblastoma glioma somatic cell hybrids. Expression of neuronal characteristics stimulated by dibutyryl adenosine 3′, 5′ cyclic monophosphate. J Cell Biol 63:691-699.
9. Dedhar S, Rennie PS, Shago M, Hagesteijn CY, Yang H, Filmus J, Hawley RG, Bruchovsky N, Cheng H, Matusik RJ, Giguere V. 1994. Inhibition of nuclear hormone receptor activity by calreticulin. Nature 367:480-483.
10. Deveraux Q, Ustrell V, Pickart C, Rechsteiner M. 1994. A 26S protease subunit that binds ubiquitin conjugates. J Biol Chem 269:7059-7061.
11. Eggleton P, Lieu TS, Zappi EG, Sastry K, Coburn J, Zaner KS, Sontheimer RD, Capra JD, Ghebrehiwet B, Tauber AI. 1994. Calreticulin is released from activated neutrophils and binds to C1q and mannan-binding protein. Clin Immunol Immunopathol 72:405-409.
12. Fliegel L, Burns K, MacLennan DH, Reithmeier RAF, Michalak M. 1989. Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum. J Biol Chem 264:21522-21528.
13. Gray AJ, Park PW, Broekelmann TJ, Laurent GJ, Reeves JT, Stenmark KR, Mecham RP. 1995. The mitogenic effects of ß-chain of fibrinogen are mediated through cell surface calreticulin. J Biol Chem 270:26602-26606.
14. Gropper R, Brandt RA, Elias S, Bearer CF, Mayer A, Schwartz AL, Ciechanover A. 1991. The ubiquitin-activating enzyme, E1, is required for stress-induced lysosomal degradation of cellular proteins. J Biol Chem 266:3602-3610.
15. Hebert DN, Foellmer B, Helenius A. 1996. Calnexin and calreticulin promote folding, delay oligomerization and suppress degradation of influenza hemagglutinin in microsomes. EMBO J 15:2961-2968.
16. Hershko A, Ciechanover A. 1992. The ubiquitin system for protein degradation. Annu Rev Biochem 61:761-807.
17. Johannes L, Tenza D, Antony C, Goud B. 1997. Retrograde transport of KDEL-bearing B-fragment of Shiga toxin. J Biol Chem 272: 19554-19561.
18. Johnson RJ, Liu N, Shanmugaratnam J, Fine RE. 1998. Increased calreticulin stability in differentiated NG-108-15 cells correlates with resistance to apoptosis induced by antisense treatment. Mol Brain Res 53:104-111.
19. Kawashima T, Zappi EG, Lieu TS, Sontheimer RD. 1994. Impact of ultraviolet radiation on the cellular expression of Ro/SS-A autoantigenic polypeotides. Dermatology 189:6-10.
20. Kettelhut IC, Wing SS, Goldberg AL. 1988. Endocrine regulation of protein breakdown is skeletal muscle. Diabetes Metab Rev 4:751-772.
21. Krause K-H, Michalak M. 1997. Calreticulin. Cell 88:439-443.
22. Letourneur F, Klausner RD. 1992. A novel di-leucine motif and a tyrosine-based motif independently mediated lysosomal targeting and endocytosis of CD3 chains. Cell 69:1143-1157.
23. 2+ response to bradykinin and increases sensitivity to ionomycin in NG108-15 cells. J Biol Chem 269:28635-28639.
24. Luskin MB, Pearlman AL, Sanes JR. 1988. Cell lineage in the cerebral cortex of the mouse studied in vivo and in vitro with a recombinant retrovirus. Neuron 1:635-647.
25. MacDonnell JM, Jones GE, White TK, Tanzer ML. 1996. Calreticulin binding affinity for glycosylated laminin. J Biol Chem 271:7891-7894.
26. Mesaeli N, Nakamura K, Zvaritch E, Dickie P, Dziak E, Krause K-H, Opas M, MacLennan DH, Michalak M. 1999. Calreticulin is essential for cardiac development. J Cell Biol 144:857-868.
27. Michalak M, Baksh S, Opas M. 1991. Identification and immunolocation of calreticulin in pancreatic cells: no evidence for "calcio-somes." Exp Cell Res 197:91-99.
28. Michalak M, Milner RE, Burns K, Opas M. 1992. Calreticulin. Biochem J 285:681-692.
29. Michalak M, Burns K, Andrin C, Mesaeli N, Jass GH, Busaan J, Opas M. 1996. Endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression. J Biol Chem 271: 29436-29445.
30. Munro S, Pelham HR. 1987. A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899-907.
31. Nash PD, Opas M, Michalak M. 1994. Calreticulin, not just another calcium-binding protein. Mol Cell Biochem 135:71-78.
32. Nauseef WM, McCormick SJ, Clark RA. 1995. Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase. J Biol Chem 270:4741-4747.
33. Oliver JD, Hresko RC, Mueckler M, High S. 1996. The glut 1 glucose transporter interacts with calnexin and calreticulin. J Biol Chem 271:13691-13696.
34. Opas M, Dziak E, Fliegel L, Michalak M. 1991. Regulation of expression and intracellular distribution of calreticulin, a major calcium binding protein of nonmuscle cells. J Cell Physiol 149:160-171.
35. Ostwald TJ, MacLennan DH. 1974. Isolation of a high affinity calcium-binding protein from sarcoplasmic reticulum. J Biol Chem 249:974-979.
36. Ostwald TJ, MacLennan DH, Dorrington KJ. 1974. Effects of cation binding on the conformation of calsequestrin and the high affinity calcium-binding protein of sarcoplasmic reticulum. J Biol Chem 249:5867-5871.
37. Otteken A, Moss B. 1996. Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin. J Biol Chem 271:97-103.
38. Pearlman AL, Sheppard AM. 1996. Extracellular matrix in early cortical development. In: Mize RR, Erzurumlu RS, editors. Progress in brain research, Vol. 108. Amsterdam: Elsevier Science, p. 119-134.
39. Peterson JR, Ora A, Van PN, Helenius A. 1995. Transient, lectin-like association of calreticulin with folding intermediates of cellular and viral glycoproteins. Mol Biol Cell 6:1173-1184.
40. Pickart CM. 1997. Targeting of substrates to the 26S proteasome. FASEB J 11:1055-1066.
41. Rajagopalan S, Xu Y, Brenner MB. 1994. Retention of unassembled components of integral membrane proteins by calnexin. Science 263:387-390.
42. Rock KL, Gramm C, Rothstein L, Clark K, Stein R, Dick L, Hwang D, Goldberg AL. 1994. Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules. Cell 78:761-771.
43. Rojian MV, Finlay BB, Gray V, Dedhar S. 1991. In vitro interaction of a polypeptide homologous to human Ro/SS-A antigen (calreticulin) with a highly conserved amino acid sequence in the cytoplasmic domain of integrin a subunit. Biochemistry 30: 9859-9866.
44. Sommer T, Wolf DH. 1997. Endoplasmic reticulum degradation: reverse protein flow of no return. FASEB J 11:1227-1233.
45. Tharin S, Dziak E, Michalak M, Opas M. 1992. Widespread tissue distribution of rabbit calreticulin, a non-muscle functional analogue of calsequestrin. Cell Tissue Res 269:29-37.
46. Unger J, Ketelbant P, Dumont JE. 1985. Inhibition by lysosomotropic amines of dog thyroid secretion in vivo. Endocrinology 116:958-965.
47. Wada I, Imai S, Kai M, Sakanea F, Kanoh H. 1995. Chaperone function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms. J Biol Chem 270:20298-20304.
48. Weist DL, Bhandoola A, Punt J, Kreibich G, McKean D, Singer A. 1997. Incomplete endoplasmic reticulum (ER) retention in immature thymocytes as revealed by surface expression of "ER-resident" molecular chaperones. Proc Natl Acad Sci USA 94:1884-1889.
49. White TK, Zhu Q, Tanzer ML. 1995. Cell surface calreticulin is a putative mannoside lectin which triggers mouse melanoma cell spreading. J Biol Chem 270:15926-15929.
50. Xiao G, Chung T-F, Pyun HY, Fine RE, Johnson RJ. 1999. KDEL proteins are found on the surface of NG108-15 cells. Mol Brain Res (in press).