Role of N-glycosylation in cathepsin E. A comparative study of cathepsin E with distinct N-linked oligosaccharides and its nonglycosylated mutant

European Journal of Biochemistry

Volumn 266, Issue 2, 1999, Pages 383-391

  Yasuda, Yoshiyuki ^a   Ikeda, Shinobu ^b   Sakai, Hideaki ^b   Tsukuba, Takayuki ^a   Okamoto, Kuniaki ^a   Nishishita, Kazuhisa ^b   Akamine, Akifumi ^a   Kato, Yuzo ^b   Yamamoto, Kenji ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b Department of Pharmacology ^*

Author keywords

Aspartic proteinase; Cathepsin E; Intracellular stability; Role of N gycosylation

Indexed keywords

ADENOSINE TRIPHOSPHATE; ASPARAGINE; ASPARAGINE LINKED OLIGOSACCHARIDE; ASPARTIC ACID; ASPARTIC PROTEINASE; CATHEPSIN E; GLUTAMINE; MANNOSE; MERCAPTOETHANOL; MUTANT PROTEIN; RECOMBINANT ENZYME; AMIDASE; CARBOHYDRATE; CATHEPSIN; CATHEPSIN F; DNA; GLYCOPEPTIDASE; LECTIN; N ACETYL BETA GLUCOSAMINIDASE; OLIGOSACCHARIDE; SIALIDASE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; ENZYME STABILITY; HUMAN; HUMAN CELL; KIDNEY CELL; NONHUMAN; OXIDATION REDUCTION STATE; PH; PRIORITY JOURNAL; PROTEIN FOLDING; RAT; SITE DIRECTED MUTAGENESIS; CHEMISTRY; COMPARATIVE STUDY; DIMERIZATION; DOSE RESPONSE; GENETIC TRANSFECTION; GLYCOSYLATION; HYDROLYSIS; IMMUNOBLOTTING; KINETICS; METABOLISM; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE; TEMPERATURE; TIME;

ADENOSINE TRIPHOSPHATE; AMIDOHYDROLASES; ASPARTIC ENDOPEPTIDASES; CARBOHYDRATES; CATALYSIS; CATHEPSIN E; CATHEPSINS; DIMERIZATION; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; IMMUNOBLOTTING; KINETICS; LECTINS; MANNOSYL-GLYCOPROTEIN ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; MUTAGENESIS, SITE-DIRECTED; MUTATION; NEURAMINIDASE; OLIGOSACCHARIDES; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; PROTEIN STRUCTURE, TERTIARY; TEMPERATURE; TIME FACTORS; TRANSFECTION;

EID: 0033485710     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00863.x     Document Type: Article

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