Nanofiltration for purification of nucleotide sugars

Journal of Carbohydrate Chemistry

Volumn 18, Issue 1, 1999, Pages 41-49

  Dudziak, Gregor ^a   Sven, Fey ^a   Hasbach, Lutz ^a   Kragl, Udo ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0033475074     PISSN: 07328303     EISSN: None     Source Type: Journal    
DOI: 10.1080/07328309908543977     Document Type: Article

References (24)

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17. 2, 1.75 U/mL inorganic pyrophosphatase, 0.2 U/mL CMP-Neu5Ac synthetase, pH 8, 30 °C, V = 450 mL, 60% yield.
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20. R = 6 mL (3 mL per vessel), membrane: YM 10 (Amicon), 98% yield.
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22. Proteins were removed in both cases by ultrafiltration using a YM 10 membrane (Amicon). CMP-Neu5Ac: Dowex 1x2 resin, column: 200 mm x 26 mm, 4 °C, pH 7.5, flow rates: 30 mL/h (sample), 60 mL/h (50 mM Na-formate), 120 mL/h (250 mM Naformate, elution of CMP-Neu5Ac). GDP-Man: Dowex 1x2 resin, column: 125 mm x 20 mm, 4 °C, pH 7, flow rates: 32 mL/h (sample), 108 mL/h (150 mM LiCl), 108 mL/h (1 M LiCl, elution of GDP-Man).
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