The synthesis of Rhodobacter capsulatus HupSL hydrogenase is regulated by the two-component HupT/HupR system

Molecular Microbiology

Volumn 34, Issue 5, 1999, Pages 995-1006

  Dischert, Wanda ^a   Vignais, Paulette M ^a   Colbeau, Annette ^a  

^a CEA GRENOBLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; HYDROGENASE; INTEGRATION HOST FACTOR; MEMBRANE ENZYME; PHOSPHOTRANSFERASE; RNA POLYMERASE; SIGMA FACTOR; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; CONTROLLED STUDY; DNA FOOTPRINTING; ENHANCER REGION; ENZYME ACTIVITY; GENE EXPRESSION; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN PHOSPHORYLATION; REGULATOR GENE; RHODOBACTER CAPSULATUS; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); ENTERIC BACTERIA; ESCHERICHIA COLI; RHODOBACTER CAPSULATUS; RHODOPSEUDOMONAS;

EID: 0033456155     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01660.x     Document Type: Article

References (51)

1. Alberti, M., Burke, D.H., and Hearst, J.E. (1995) Structure and sequence of the photosynthetic gene cluster. In Anoxygenic Photosynthetic Bacteria Blankenship, R.E., Madigan, M.T., and Bauer, C.E. (eds). Dordrecht: Kluwer Academic Publishers, pp. 1083-1106.
2. Black, L.K., and Maier, R.J. (1995) IHF-and RpoN-dependent regulation of hydrogenase expression in Bradyrhizobium japonicum. Mol Microbiol 16: 405-413.
3. Black, L.K., Fu, C., and Maier, R.J. (1994) Sequence and characterization of hupU and hupV genes of Bradyrhizobium japonicum encoding a possible nickel-sensing complex involved in hydrogenase expression. J Bacteriol 176: 7101-7106.
4. Bowman, W.C., and Kranz, R.G. (1998) A bacterial ATP-dependent, enhancer binding protein that activates the housekeeping RNA polymerase. Genes Dev 12: 1884-1893.
5. Brito, B., Martinez, M., Fernandez, D., Rey, L., Cabrera, E., Palacios, J.M., et al. (1997) Hydrogenase genes from Rhizobium leguminosarum bv. viciae are controlled by the nitrogen fixation regulatory protein NifA. Proc Natl Acad Sci USA 94: 6019-6024.
6. Cangelosi, G.A., and Wheelis, M.L. (1984) Regulation by molecular oxygen and organic substrates of hydrogenase synthesis in Alcaligenes eutrophus. J Bacteriol 159: 138-144.
7. Colbeau, A., and Vignais, P.M. (1983) The membrane-bound hydrogenase of Rhodopseudomonas capsulata is inducible and contains nickel. Biochim Biophys Acta 748: 128-138.
8. 2. J Bacteriol 174: 4258-4264.
9. Colbeau, A., Kelley, B.C., and Vignais, P.M. (1980) Hydrogenase activity in Rhodopseudomonas capsulata: Relationship with nitrogenase activity. J Bacteriol 144: 141-148.
10. Colbeau, A., Richaud, P., Toussaint, B., Caballero, J., Elster, C., Delphin, C., et al. (1993) Organization of the genes necessary for hydrogenase expression in Rhodobacter capsulatus. Sequence analysis and identification of two hyp regulatory mutants. Mol Microbiol 8: 15-29.
11. Colbeau, A., Kovacs, K.L., Chabert, J., and Vignais, P.M. (1994) Cloning and sequences of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase synthesis in Thiocapsa roseopersicina. Gene 140: 25-31.
12. Ditta, G., Schmidhauser, T., Yakobson, E., Ju, P., Liang, X.W., Finlay, D.R., et al. (1985) Plasmids related to the broad host range vector, pRK290, useful for gene cloning and monitoring gene expression. Plasmid 13: 149-153.
13. 2 and carbon substrates in Alcaligenes latus. J Bacteriol 169: 4463-4468.
14. Drummond, M.H., Whitty, P., and Wooton, J.C. (1986) Sequence and domain relationship of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. EMBO J 5: 441-447.
15. Duport, C., Meyer, C., Naud, I., and Jouanneau, Y. (1994) A new gene expression system based on a fructose-dependent promoter from Rhodobacter capsulatus. Gene 145: 103-108.
16. Durmowicz, M.C., and Maier, R.J. (1997) Roles of HoxX and HoxA in biosynthesis of hydrogenase in Bradyrhizobium japonicum. J Bacteriol 179: 3676-3682.
17. Durmowicz, M.C., and Maier, R.J. (1998) The FixK2 protein is involved in regulation of symbiotic hydrogenase expression in Bradyrhizobium japonicum. J Bacteriol 180: 3253-3256.
18. Eberz, G., and Friedrich, B. (1991) Three trans-acting functions control hydrogenase expression in Alcaligenes eutrophus. J Bacteriol 173: 1845-1854.
19. Elsen, S. (1996) Rôle de l'opéron hupTUV dans la répression de la synthèse de l'hydrogénase chez Rhodobacter capsulatus. Grenoble, France: PhD Thesis.
20. Elsen, S., Richaud, P., Colbeau, A., and Vignais, P.M. (1993) Sequence analysis and interposon mutagenesis of the hupT gene, which encodes a sensor protein involved in repression of hydrogenase synthesis in Rhodobacter capsulatus. J Bacteriol 175: 7404-7412.
21. Elsen, S., Colbeau, A., Chabert, J., and Vignais, P.M. (1996) The hupTUV operon is involved in negative control of hydrogenase synthesis in Rhodobacter capsulatus. J Bacteriol 178: 5174-5181.
22. Elsen, S., Colbeau, A., and Vignais, P.M. (1997) Purification and in vitro phosphorylation of HupT, a regulatory protein controlling hydrogenase gene expression in Rhodobacter capsulatus. J Bacteriol 179: 968-971.
23. Foster-Hartnett, D., Cullen, P.J., Monika, E.M., and Kranz, R.G. (1994) A new type of NtrC transcriptional activator. J Bacteriol 176: 6175-6187.
24. Friedrich, B., and Schwartz, E. (1993) Molecular biology of hydrogen utilization in aerobic chemolithotrophes. Ann Rev Microbiol 47: 351-383.
25. Friedrich, C.G., Friedrich, B., and Bowien, A. (1981) Formation of enzymes of autotrophic metabolism during heterotrophic growth of Alcaligenes eutrophus. J Gen Microbiol 122: 69-78.
26. Goudreau, P.N., and Stock, A.M. (1998). Signal transduction in bacteria: molecular mechanisms of stimulus-response coupling. Curr Opin Microbiol 1: 160-169.
27. Hidalgo, E., Leyva, A., and Ruiz-Argüeso, T. (1990) Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum. Plant Mol Biol 15: 367-370.
28. Hübner, P., Willison, J.C., Vignais, P.M., and Bickle, T.A. (1991) Expression of regulatory nif genes in Rhodopseudomonas capsulata. J Bacteriol 173: 2993-2999.
29. Jones, R., and Haselkorn, R. (1989) The DNA sequence of the Rhodobacter capsulatus ntrA, ntrB and ntrC analogues required for nitrogen fixation. Mol Gen Genet 217: 507-516.
30. 54 gene (rpoN). J Bacteriol 173: 1125-1138.
31. 2 sensing in Alcaligenes eutrophus. Proc Natl Acad Sci USA 95: 12474-12479.
32. Lenz, O., Strack, A., Tran-Betcke, A., and Friedrich, B. (1997) A hydrogen sensing system in transcriptional regulation of hydrogenase expression in Alcaligenes species. J Bacteriol 179: 1655-1663.
33. Marrs, B. (1974) Genetic recombinaison in Rhodopseudomonas capsulata. Proc Natl Acad Sci USA 71: 971-973.
34. Maxam, A.M., and Gilbert, W. (1980). Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65: 499-560.
35. Parkinson, J.S., and Kofoid, E.C. (1992) Communication modules in bacterial signaling proteins. Annu Rev Genet 26: 71-112.
36. Prosser, J., Graham, L., and Maier, R.J. (1988) Hydrogen-mediated enhancement of hydrogenase expression in Azotobacter vinelandii. J Bacteriol 170: 1990-1993.
37. Richaud, P., Colbeau, A., Toussaint, B., and Vignais, P.M. (1991) Identification and sequence analysis of the hupR gene which encodes a response regulator of the NtrC family required for hydrogenase expression in Rhodobacter capsulatus. J Bacteriol 173: 5298-5392.
38. Römermann, D., Warrelmann, J., Bender, R.A., and Friedrich, B. (1989) An rpoN-like gene of Alcaligenes eutrophus and Pseudomonas facilis controls the expression of diverse metabolic pathways, including hydrogen oxidation. J Bacteriol 171: 1093-1099.
39. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, NY: Cold Spring, Harbor Laboratory Press.
40. Schröder, I., Wolin, C.D., Caricchioli, R., and Gunsalus, R.P. (1994). Phosphorylation and dephosphorylation of the NarQ, NarX, and NarL proteins of the nitrate-dependent two-component regulatory system of Escherichia coli. J Bacteriol 176: 4985-4992.
41. Schwartz, E., Gerischer, U., and Friedrich, B. (1998). Transcriptional regulation of Alcaligenes eutrophus hydrogenase genes. J Bacteriol 180: 3197-3204.
42. Stocker, K., Reijinders, W.N.M., Oltmann, L.F., and Stouthammer, A.H. (1989) Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K12. J Bacteriol 171: 4448-4456.
43. Toussaint, B., David, L., De Sury d'Aspremont, R., and Vignais, P.M. (1994) The IHF proteins of Rhodobacter capsulatus and Pseudomonas aeruginosa. Biochimie 76: 951-957.
44. 2 activation of hupSLC transcription. Mol Microbiol 26: 927-937.
45. Van Soom, C., Verreth, C., Sampaio, M.J., and Vanderleyden, J. (1993) Identification of a potential transcriptional regulator of hydrogenase activity in free-living Bradyrhizobium japonicum strains. Mol Gen Genet 239: 235-240.
46. Van Soom, C., de Wilde, C., and Vanderleyden, J. (1997) Hox A is a transcriptional regulator for expression of the hup structural genes in free-living Bradyrhizobium japonicum. Mol Microbiol 23: 967-977.
47. Vénien-Bryan, C., Balavoine, F., Toussaint, B., Miokowski, C., Hewat, E.A., Helme, B., and Vignais, P.M. (1997) Structural study of the response regulator HupR from Rhodobacter capsulatus. Electron microscopy of 2D crystals under a nickel-chelating lipid. J Mol Biol 274: 687-692.
48. 2-uptake hydrogenases. Arch Microbiol 161: 1-10.
49. 2: evidence from the H-D exchange reaction. J Bacteriol 179: 290-292.
50. Weaver, P.F., Wall, J.D., and Gest, H. (1975) Characterization of Rhodopseudomonas capsulata. Arch Microbiol 105: 207-221.
51. Yannish-Perron, C., Vieira, J., and Messing, J. (1985) Improved M12 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC vectors. Gene 33: 103-119.