Effects of in vitro addition of ammonia on the metabolism of 15N-labelled amino acids in isolated sheep hepatocytes

Canadian Journal of Animal Science

Volumn 79, Issue 3, 1999, Pages 321-326

  Mutsvangwa, Timothy ^a,b   Buchanan Smith, Jock G ^b   McBride, Brian W ^b  

^a Henderson Research Station Harare   (Zimbabwe)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

15N; Amino acids; Ammonia; Liver cells; Sheep; Ureagenesis

Indexed keywords

OVIS ARIES;

EID: 0033455040     PISSN: 00083984     EISSN: None     Source Type: Journal    
DOI: 10.4141/A98-082     Document Type: Article

References (26)

1. Armentano, L. E. 1994. Impact of metabolism by extragastrointestinal tissues on secretory rate of milk proteins. J. Dairy Sci. 77: 2809-2820.
2. Association of Official Analytical Chemists. 1984. Official methods of analysis. 14th ed. AOAC, Washington, DC.
3. Bergman, E. N. and Pell, J. M. 1985. Integration of amino acid metabolism in the ruminant. Pages 613-628 in F. M. C. Gilchrist and R. I. MacKie, ed. Herbivore nutrition in the subtropics and tropics. Science Press, Johannesburg, South Africa.
4. Black, A. L., Anand, R. S., Bruss, M. L., Brown, C. A. and Nakagiri, J. A. 1990. Partitioning of amino acids in lactating cows: oxidation to carbon dioxide. J. Nutr. 120: 700-710.
5. Demigné, C., Yacoub, C., Morand, C. and Rémésy, C. 1991. Interactions between propionate and amino acid metabolism in isolated sheep hepatocytes. Br. J. Nutr. 65: 301-317.
6. Fitch, N. A., Gill, M., Lomax, M. A. and Beever, D. E. 1989. Nitrogen and glucose metabolism by the liver of forage and forage-concentrate fed cattle. Proc. Nutr. Soc. 48: 76A (Abstr.).
7. Goering, H. K. and Van Soest, P. J. 1970. Forage fibre analysis: apparatus, reagents, procedures and some applications. USDA Agricultural Handbook 379. Washington, DC.
8. Häussinger, D. 1990. Nitrogen metabolism in the liver: structural and functional organization and physiological relevance. Biochem. J. 267: 281-290.
9. Huntington, G. B. 1986. Uptake and transport of nonprotein nitrogen by the ruminant gut. Fed. Proc. 45: 2272-2276.
10. Lobley, G. E., Connell, A., Lomax, M. A., Brown, D. S., Milne, E., Calder, A. G. and Farningham, D. A. H. 1995. Hepatic detoxification of ammonia in the ovine liver: possible consequences for amino acid catabolism. Br. J. Nutr. 73: 667-685.
11. Lobley, G. E., Weijs, P. J. M., Connell, A., Calder, A. G., Brown, D. S. and Milne, E. 1996. The fate of absorbed and exogenous ammonia as influenced by forage or forage-concentrate diets in growing sheep. Br. J. Nutr. 76: 231-248.
12. Looney, M. C., Baldwin, R. L. and Calvert, C. C. 1987. Gluconeogenesis in isolated lamb hepatocytes. J. Anim. Sci. 64: 283-294.
13. 4Cl in isolated sheep hepatocytes. Eur. J. Biochem. 228: 912-917.
14. Maltby, S. A., Lomax, M. A., Beever, D. E. and Pippard, C. J. 1991. The effect of increased ammonia and amino acid supply on postprandial portal-drained viscera and hepatic metabolism in growing steers fed maize silage. Page 20 in C. Wenk and M. Boessinger, ed. Proceedings of the 12th Symposium on Energy Metabolism of Farm Animals, EAAP Publ. No. 58. ETH-Zentrum, Zurich, Switzerland.
15. McBride, B. W. and Milligan, L. P. 1985. Magnitude of ouabain-sensitive respiration in the liver of growing, lactating and starved sheep. Br. J. Nutr. 65: 293-303.
16. Mutsvangwa, T., Buchanan-Smith, J. G. and McBride, B. W. 1996. Interactions between ruminal degradable nitrogen intake and in vitro addition of substrates on patterns of amino acid metabolism in isolated ovine hepatocytes. J. Nutr. 126: 209-218.
17. 15N]alanine in isolated sheep hepatocytes. J. Anim. Sci. 75: 1149-1159.
18. 15N. Arch. Biochem. Biophys. 292: 393-401.
19. 15N enrichment in multiple amino acids and urea in a single analysis by gas chromatography/mass spectrometry. Biol. Mass Spectr. 22: 518-523.
20. Pell, J. M., Caldarone, E. M. and Bergman, E. N. 1986. Leucine and α-ketoisocaproate metabolism and interconversions in fed and fasted sheep. Metabolism 35: 1005-1016.
21. Reynolds, C. K., Tyrell, H. F. and Reynolds, P. J. 1991. Effects of diet forage-to-concentrate ratio and intake on energy metabolism in growing beef heifers: net nutrient metabolism by visceral tissues. J. Nutr. 121: 1004-1015.
22. Reynolds, C. K. 1992. Metabolism of nitrogenous compounds by ruminant liver. J. Nutr. 122: 850-854.
23. SAS Institute, Inc. 1985. SAS user's guide: Statistics. SAS Institute, Inc., Cary, NC.
24. 15N]urea in plasma and application to urea metabolism study. Anal. Chem. 54: 489-491.
25. 15N]ammonia into urea and amino acids in humans and the effect of nutritional status. Br. J. Nutr. 76: 491-499.
26. Wolff, J. E., Bergman, E. N. and Williams, H. H. 1972. Net metabolism of plasma amino acids by liver and portal-drained viscera of fed sheep. Am. J. Physiol. 223: 438-446.