Quantification of apoptosis induction by doxorubicin in three types of human mammary carcinoma spheroids

Anticancer Research

Volumn 19, Issue 4 B, 1999, Pages 3141-3146

  Dunkern, Torsten R ^a   Mueller Klieser, Wolfgang ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Apoptosis; Cell cycle; Doxorubicin; Mammary carcinoma; Spheroids; TUNEL

Indexed keywords

CELL DNA; DOXORUBICIN;

APOPTOSIS; ARTICLE; BREAST CARCINOMA; CARCINOMA CELL; CELL CYCLE; CELL STRAIN MCF 7; CHEMOSENSITIVITY; CLONOGENESIS; CONTROLLED STUDY; DNA SYNTHESIS; DRUG EFFECT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MONOLAYER CULTURE; PRIORITY JOURNAL; TUMOR SPHEROID;

APOPTOSIS; BREAST NEOPLASMS; CELL CYCLE; CELL DIVISION; DOXORUBICIN; HUMANS; IN SITU NICK-END LABELING; TUMOR CELLS, CULTURED;

EID: 0033427560     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. McConkey DJ, Greene G and Pettaway CA: Apoptosis resistance increases with metastatic potential in cells of the human LNCaP prostate carcinoma line. Cancer Res 56: 5594-5599, 1996.
2. Milas L, Stephens LC and Meyn R: Relation of apoptosis to cancer therapy. In Vivo 8: 665-673, 1994.
3. Tsujimoto Y, Cossman J, Jaffe E, and Croce CM: Involvement of the bcl-2 oncogene in human follicular lymphoma. Science 228: 1440-1443, 1985.
4. Lutzker SG and Levine AJ: Apoptosis and cancer chemotherapy. Cancer Treat Res 87: 345-356, 1996.
5. Mueller-Klieser W: Multicellular spheroids. A review on cellular aggregates in cancer research. J Cancer Res Clin Oncol 113: 101-122, 1987.
6. Mueller-Klieser W: Three-dimensional cell cultures: from molecular mechanisms to clinical applications. Am J Physiol 273: C1109-C1123, 1997.
7. Sutherland R.M: Cell and environment interactions in tumor microregions: the multicell spheroid model. Science 240: 177-184, 1988.
8. Durand RE: Flow cytometry studies of intracellular adriamycin in multicell spheroids in vitro. Cancer Res 41: 3495-3498, 1981.
9. Nederman T, Carlsson J and Malmquvist M: Penetration of substances into tumour tissue. A methodological study on cellular spheroids. In Vitro 17: 290-298, 1981.
10. Teicher BA, Kobayashi H, Man S, Graham CH, Kapitain SJ and Kerbel RS: Acquired multicellular-mediated resistance to alkylating agents in cancer. Proc Natl Acad Sci USA 90: 3294-3298, 1993.
11. Gavrielli Y, Sherman Y and Ben-Sasson SA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119: 494-501, 1992.
12. Bosch I and Croop J: P-glycoprotein multidrug resistance and cancer. Biochem Biophys Acta 1288: F37-F54, 1996.
13. Winsor CP: The Gompertz curve as a growth curve. Proc Natl Acad Sci 18: 1-7, 1932.
14. Compton MM: A biochemical hallmark of apoptosis: internucleosomal degradation of the genome. Canc Metas Rev 11: 105-119, 1992.
15. Gratzner HG: Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218: 474, 1982.
16. Gray JW: Monoclonal antibodies against bromodeoxyuridine. Cytometry 6: 501-673, 1985.
17. Darzynkiewicz Z: Apoptosis in antitumor strategies: modulation of cell cycle or differentiation. J Cell Biochem 58: 151-159, 1995.
18. Otto AM, Paddenberg R, Schubert S, Mannherz HG: Cell-cycle arrest, micronucleus formation, and cell death in growth inhibition of MCF-7 breast cancer cells by tamoxifen and cisplatin. J Cancer Res Clin Oncol 122: 603-612, 1996.
19. Cohen GM, Sun X.M, Snowden RM, Dinsdale D and Skilleter: Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem J 286: 331-334, 1992.
20. Collins RJ, Harmon BV, Gobe GC and Kerr JFR: Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. Int J Rad Biol 61: 451-453, 1992.
21. Concordet JP and Ferry A: Physiological programmed cell death in thymocytes is induced by physical stress (exercise). Am J Physiol 265: C626-629, 1993.
22. Bursch W, Pfaffe S, Putz B, Barthel G, and Schulte-Hermann R: Determination of the length of the histological states of apoptosis in normal liver and in altered hepati foci of rats. Carcinogenesis 11: 847-853, 1990.