Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli

Bioseparation

Volumn 8, Issue 1-5, 1999, Pages 219-228

  Brobjer, Michael ^a  

^a PFIZER INC   (United States)

Author keywords

Cation exchange chromatography; Expanded bed adsorption (EBA)

Indexed keywords

ADSORBENT; ADSORPTION; BIOMASS; CYCLIC GMP; ELUTION; EXPANDED BED ADSORPTION CHROMATOGRAPHY; HYBRID PROTEIN; PROCESS DEVELOPMENT; PROTEIN EXPRESSION; PROTEIN ISOLATION; SCALE-UP; STREAMLINE; SWEDEN;

ADSORBENTS; BIOMASS; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI; ION EXCHANGE; PROTEINS;

EXPANDED BED ADSORPTION;

ADSORPTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033402074     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-94-017-1519-5_24     Document Type: Article

References (5)

1. Andrews AT, Noble I, Keeratipibul S, and Asenjo, JA (1994) Physicochemical properties of the main matrix proteins of three important culture vehicles. Biotechnol. Bioeng., 44 29-37.
2. Barnfield Frej A-K, Hjorth R and Hammarström Å (1994) Pilot scale recovery of recombinant Annexin V from unclarified Escherichia coli homogenate using expanded bed adsorption, Biotechnol. Bioeng., 44 922-929.
3. Chase HA (1994) Purification of proteins by adsorption chromatography in expanded beds. Trends in biotech. 12: 296-303.
4. Draeger MN and Chase HA (1991) Liquid fluidized bed adsorption of proteins in the presence of cells. Bioseparations 2: 67-80
5. Hedman P and Barnfield Frej A-K (1992) Adapting chromatography for initial large-scale protein recovery. In: Ladisch M and Bose A (eds) ACS Conference Proceedings Series, Harnessing Biotechnology for the 21st century, pp 271-274.