Indispensability of transmembrane domains of Golgi UDP-galactose transporter as revealed by analysis of genetic defects in UDP-galactose transporter-deficient murine Had-1 mutant cell lines and construction of deletion mutants

Journal of Biochemistry

Volumn 126, Issue 6, 1999, Pages 1107-1117

  Ishida, Nobuhiro ^a   Yoshioka, Shigemi ^a   Iida, Mai ^a   Sudo, Kozue ^a   Miura, Nobuhiko ^a,b   Aoki, Kazuhisa ^a   Kawakita, Masao ^a  

^a TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

FISH; Green fluorescent protein; Nucleotide sugar transporter; Structure function relationships; UDP galactose transporter

Indexed keywords

CARRIER PROTEIN; GALACTOSYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; NUCLEOTIDE; URIDINE DIPHOSPHATE GALACTOSE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GOLGI COMPLEX; HAMSTER; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; MEMBRANE; MOLECULAR CLONING; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; STRUCTURE ACTIVITY RELATION;

ANIMALIA; CRICETINAE; MURINAE;

EID: 0033401292     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022556     Document Type: Article

References (26)

1. Kawakita, M., Ishida, N., Miura, N., Sun-Wada, G.-H., and Yoshioka, S. (1998) Nucleotide sugar transporters: elucidation of their molecular identity and its implication for future studies. J. Biochem. 123, 777-785
2. Hirschberg, C.B., Robbins, P.W., and Abeijon, C. (1998) Transporters of nucleotide sugars, ATP, and nucleotide sulfate in the endoplasmic reticulum and Golgi apparatus. Annu. Rev. Biochem. 67, 49-69
3. Miura, N., Ishida, N., Hoshino, M., Yamauchi, M., Hara, T., Ayusawa, D., and Kawakita, M. (1996) Human UDP-galactose translocator: Molecular cloning of a complementary DNA that complements the genetic defect of a mutant cell line deficient in UDP-galactose translocator. J. Biochem. 120, 236-241
4. Ishida, N., Miura, N., Yoshioka, S., and Kawakita, M. (1996) Molecular cloning and characterization of a novel isoform of the human UDP-galactose transporter, and of related complementary DNAs belonging to the nucleotide-sugar transporter gene family. J. Biochem. 120, 1074-1078
5. Segawa, H., Ishida, N., Takegawa, K., and Kawakita, M. (1999) Schizosaccharomyces pombe UDP-galactose transporter: identification of its functional form through cDNA cloning and expression in mammalian cells. FEBS Lett. 451, 295-298
6. Ishida, N., Ito, M., Yoshioka, S., Sun-Wada, G.-H., and Kawakita, M. (1998) Functional expression of human Golgi CMP-sialic acid transporter in the Golgi complex of a transporter-deficient Chinese hamster ovary cell mutant. J. Biochem. 124, 171-178
7. Eckhardt, M., Mühlenhoff, M., Bethe, A., and Gerardy-Schahn, R. (1996) Expression cloning of the Golgi CMP-sialic acid transporter. Proc. Natl. Acad. Sci. USA 93, 7572-7576
8. Eckhardt, M. and Gerardy-Schahn, R. (1997) Molecular cloning of the hamster CMP-sialic acid transporter. Eur. J. Biochem. 248, 187-192
9. Ishida, N., Yoshioka, S., Chiba, Y., Takeuchi, M., and Kawakita, M. (1999) Molecular cloning and functional expression of the human Golgi UDP-N-acetylglucosamine transporter. J. Biochem. 126, 68-77
10. Guillen, E., Abeijon, C., and Hirschberg, C.B. (1998) Mammalian Golgi apparatus UDP-N-acetylglucosamine transporter: molecular cloning by phenotypic correction of a yeast mutant. Proc. Natl. Acad. Sci. USA 95, 7888-7892
11. Abeijon, C., Robbins, P.W., and Hirschberg, C.B. (1996) Molecular cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter from Kluyveromyces lactis. Proc. Natl. Acad. Sci. USA 93, 5963-5968
12. Descoteaux, A., Luo, Y., Turco, S.J., and Beverley, S.M. (1995) A specialized pathway affecting virulence glycoconjugates of Leishmania. Science 269, 1869-1872
13. Ma, D.Q., Russell, D.G., Beverley, S.M., and Turco, S.J. (1997) Golgi GDP-mannose uptake requires Leishmania LPG2: A member of a eukaryotic family of putative nucleotide-sugar transporters. J. Biol. Chem. 272, 3799-3805
14. Poster, J.B. and Dean, N. (1996) The yeast VRG4 gene is required for normal Golgi functions and defines a new family of related genes. J. Biol. Chem. 271, 3837-3845
15. Dean, N., Zhang, Y.B., and Poster, J.B. (1997) The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae. J. Biol. Chem. 272, 31908-31914
16. Eckhardt, M., Gotza, B., and Gerardy-Schahn, R. (1999) Membrane topology of the mammalian CMP-sialic acid transporter. J. Biol. Chem. 274, 8779-8787
17. Stanley, P. (1981) Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes. Mol. Cell. Biol. 1, 687-696
18. Nakano, N. (1966) Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium. Tohoku J. Exp. Med. 88, 69-84
19. Hara, T., Hattori, S., and Kawakita, M. (1989) Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease virus receptors. J. Virol. 63, 182-188
20. Deutscher, S.L. and Hirschberg, C.B. (1986) Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes. J. Biol. Chem. 261, 96-100
21. Hara, T., Endo, T., Furukawa, K., Kawakita, M., and Kobata, A. (1989) Elucidation of the phenotypic change on the surface of Had-1 cell, a mutant cell line of mouse FM3A carcinoma cells selected by resistance to Newcastle disease virus infection. J. Biochem. 106, 236-247
22. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467
23. Yoshioka, S., Sun-Wada, G.-H., Ishida, N., and Kawakita, M. (1997) Expression of the human UDP-galactose transporter in the Golgi membranes of murine Had-1 cells that lack the endogenous transporter. J. Biochem. 122, 691-695
24. Hara, T., Yamauchi, M., Takahashi, E., Hoshino, M., Aoki, K., Ayusawa, D., and Kawakita, M. (1993) The UDP-galactose translocator gene is mapped to band Xp11.23-p11.22 containing the Wiskott-Aldrich syndrome locus. Somat. Cell Mol. Gen. 19, 571-575
25. Stanley, P. and Siminovitch, L. (1977) Complementation between mutants of CHO cells resistant to a variety of plant lectins. Somat. Cell Genet. 3, 391-405
26. Aoki, K., Sun-Wada, G.-H., Segawa, H., Yoshioka, S., Ishida, N., and Kawakita, M. (1999) Expression and activity of chimeric molecules between human UDP-galactose transponer and CMP-sialic acid transporter. J. Biochem. 126, 940-950