Establishment and characterization of an immortalized human sebaceous gland cell line (SZ95)

Journal of Investigative Dermatology

Volumn 113, Issue 6, 1999, Pages 1011-1020

  Zouboulis, Christos C ^a   Seltmann, Holger ^a   Neitzel, Heidemarie ^b   Orfanos, Constantin E ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Androgens; Cell transformation; Polyomavirus transforming; Retinoids; Viral antigens

Indexed keywords

ANDROGEN; KERATIN; VIRUS ANTIGEN;

ARTICLE; CELL IMMORTALIZATION; CELL LINE; CELL PROLIFERATION; CELL SIZE; CELL TRANSFORMATION; DESMOSOME; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; POLYOMA VIRUS; PRIORITY JOURNAL; SEBACEOUS GLAND;

EID: 0033397124     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1747.1999.00771.x     Document Type: Article

References (60)

1. Ades EW, Candal FJ, Swerlick RW, George VG, Summers S, Bosse DC, Lawley TJ: HMEC-1: Establishment of an immortalized human microvascular endothelial cell line. J Invest Dermatol 99:683-690, 1992
2. Akai Y, Akamatsu H, Ri S, Ito A, Zouboulis ChC, Asada Y: Influence of free fatty acids on the proliferation of cultured human sebocytes in vitro. Jpn J Dermatol 104:647-649, 1994
3. Akamatsu H, Zouboulis ChC, Orfanos CE: Control of human sebocyte proliferation in vitro by testosterone and 5-alpha-dihydrotestosterone is dependent on the localization of the sebaceous glands. J Invest Dermatol 99:509-511, 1992
4. Bae VL, Jackson-Cook CK, Maygarden SJ, Plymate SR, Chen J, Ware JL: Metastatic sublines of a SV40 large T antigen immortalized human prostate epithelial cell line. Prostate 34:275-282, 1998
5. Baumrind NL, Parkinson D, Wayne DB, Heuser JE, Pearlman A: EMA: a developmentally regulated cell-surface glycoprotein of CNS neurons that is concentrated at the leading edge of growth cones. Dev Dyn 194:311-325, 1992
6. Boyce ST, Ham RG: Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol 81(Suppl.):33s-40s, 1983
7. Brown SK, Shalita AR: Acne vulgaris. Lancet 351:1871-1876, 1998
8. Chang TH, Ray FA, Thompson DA, Schlegel R: Disregulation of mitotic checkpoints and regulatory proteins following acute expression of SV40 large T antigen in diploid human cells. Oncogene 14:2383-2393, 1997
9. Chen W, Zouboulis ChC, Fritsch M, et al: Evidence of heterogeneity and quantitative differences of the type 1 5α-reductase expression in cultured human skin cells. Evidence of its presence in melanocytes. J Invest Dermatol 110:84-89, 1998
10. Cooper MF, McGrath H, Shuster S: Sebaceous lipogenesis in human skin. Variability with age and severity of acne. Br J Dermatol 94:165-172, 1976
11. Cunliffe WJ: The sebaceous gland and acne - 40 years on. Dermatology 196:9-15, 1998
12. De Kretser T: The ovarian-carcinoma-associated sebaceous gland antigen is a member of the HMFG2-positive family of epithelial mucins. Tumor Biol 9:154-164, 1988
13. Detmar M, Mayer-da-Silva A, Stadler R, Orfanos CE: Effects of azelaic acid on proliferation and ultrastructure of mouse keratinocytes in vitro. J Invest Dermatol 93:70-74, 1989
14. Dobbelstein M, Arthur AK, Dehde S, van Zee K, Dickmanns A, Fanning E: Intracistronic complementation reveals a new function of SV40 T antigen that co-operates with Rb and p53 binding to stimulate DNA synthesis in quiescent cells. Oncogene 7:837-847, 1992
15. Doran T, Shapiro SS: Retinoid effects on sebocyte proliferation. In: Packer L (ed.). Methods in Enzymology. San Diego: Academic Press, 1990, pp 334-338
16. Doran TI, Lucas DA, Levin AA, et al: Biochemical and retinoid receptor activities in human sebaceous cells. In: Saurat J-H (ed.). Retinoids: 10 Years On. Basel: Karger, 1991a, pp 243-253
17. Doran TI, Baff R, Jacobs P, Pacia E: Characterization of human sebaceous cells in vitro. J Invest Dermatol 96:341-348, 1991b
18. 4 isomerase in human skin. J Invest Dermatol 99:415-421, 1992
19. Dutt K, Scott M, Del Monte M, Agarwal N, Sternberg P, Srivastava SK, Srinivasan A: Establishment of human retinal pigment epithelial cell lines by oncogenes. Oncogene 5:195-200, 1990
20. Fujie T, Shikiji T, Uchida N, Urano Y, Nagae H, Arase S: Culture of cells derived from the human sebaceous gland under serum-free conditions without a biological feeder layer or specific matrices. Arch Dermatol Res 288:703-708, 1996
21. Geiger J-M: Retinoids and sebaceous gland activity. Dermatology 191:305-310, 1995
22. Gollnick H, Zouboulis ChC, Akamatsu H, Kurokawa I, Schulte A: Pathogenesis and pathogenesis related treatment of acne. J Dermatol 18:489-499, 1991
23. Harlow E, Crawford LV, Pim DC, Williamson NM: Monoclonal antibodies specific for simian virus 40 tumor antigens. J Virol 39:861-869, 1981
24. 14C by suction blister epidermis and other skin components. Proc Soc Exp Biol Med 135:285-291, 1970
25. Huang CF, Cabot MC: Phorbol diesters stimulate the accumulation of phosphatidate, phosphatidylethanol, and diacylglycerol in three cell types. Evidence for the indirect formation of phosphatidylcholine-derived diacylglycerol by a phospholipase D pathway and direct formation of diacylglycerol by a phospholipase C pathway. J Biol Chem 265:14858-14863, 1990
26. Jiang C-K, Konnolly D, Blumenberg M: Comparison of methods for transfection of human epidermal keratinocytes. J Invest Dermatol 97:969-973, 1991
27. Kaluzny MA, Duncan LA, Merritt MV, Epps DE: Rapid separation of lipid classes in high yield and purity using bonded phase columns. J Lipid Res 26:135-140, 1985
28. Kanitakis J, Al-Rifai I, Faure M, Claudy A: Thomsen-Friedenreich and its precursor (Tn) antigen expression in normal skin and in benign cutaneous tumours: a marker for sebaceous differentiation. Acta Derm Venereol (Stockh) 78:173-176, 1998
29. Karsten U, Butschak G, Cao Y, Goletz S, Hanisch FG: A new monoclonal antibody (A78-G/A7) to the Thomsen-Friedenreich pan-tumor antigen. Hybridoma 14:37-44, 1995
30. Kealey T, Lee CM, Thody AJ, Coaker T: The isolation of human sebaceous glands and apocrine sweat glands by shearing. Br J Dermatol 114:181-188, 1986
31. Lane EB, Bártek J, Purkis PE, Leigh IM: Keratin antigens in differentiating skin. Ann NY Acad Sci USA 455:241-258, 1985
32. Latham JAE, Redfern CPF, Thody AJ, de Kretser TA: Immunohistochemical markers of human sebaceous gland differentiation. J Histochem Cytochem 37:729-734, 1989
33. Lee CM: Cell culture systems for the study of human skin and skin glands. In: Jones CJ (ed.) Epithelia: Advances in Cell Physiology and Cell Culture. Dordrecht: Kluwer, 1990, pp 333-350
34. Luu-The V, Sugimoto Y, Puy L, Labrie Y, Solache IL, Singh M, Labrie F: Characterization, expression, and immunohistochemical localization of 5α-reductase in human skin. J Invest Dermatol 102:221-226, 1994
35. Milne JA: The metabolism of androgens by sebaceous glands. Br J Dermatol 81:23-28, 1969
36. Mitchell PJ, Wang C, Tjian R: Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 50:847-861, 1987
37. Nikkari T: Comparative chemistry of sebum. J Invest Dermatol 62:257-267, 1974
38. Ormerod MG, Steele K, Westwood JH, Mazzini MN: Epithelial membrane antigen: partial purification, assay and properties. Br J Cancer 48:533-541, 1983
39. Petersen MJ, Zone JJ, Krueger GG: Development of a nude mouse model to study human sebaceous gland physiology and pathophysiology. J Clin Invest 74:1358-1365, 1984
40. Pochi P: Sebaceous gland assay. In: Lowe N, Maibach H (eds). Models in Dermatology, Vol. 2. Basel: Karger, 1985, pp 70-75
41. Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P: Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170:235-249, 1987
42. Shapiro SS, Hurley J, Vane FM, Doran T: Evaluation of potential therapeutic entities for the treatment of acne. In: Reichert U, Shroot B (eds). Pharmacology of Retinoids in the Skin, Pharmacol Skin, Vol. 3. Basel: Karger, 1989, pp 104-112
43. Sharp F, Hay JB, Hodgins MB: Metabolism of androgens in vitro by human foetal skin. J Endocrinol 70:491-499, 1976
44. Staedel K, Remy J-S, Hua Z, Broker TR, Chow LT, Behr J-P: High-efficiency transfection of primary human keratinocytes with positively charged lipopolyamine:DANN complexes. J Invest Dermatol 102:768-772, 1994
45. Stähli C, Caravatti M, Aesbacher M, Kociba C, Takacs B, Carman H: A mucin-like carcinoma associated antigen (MCA) defined by three monoclonal antibodies against different epitopes. Cancer Res 48:6799-6802, 1988
46. Strauss JS: Sebaceous gland, acne and related disorders - an epiloque. Dermatology 196:182-184, 1998
47. Taylor-Papadimitriou J, Peterson JA, Arklie J, Burchell J, Ceriani RL, Bodmer WF: Monoclonal antibodies to epithelium specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer 28:17-21, 1981
48. Tohyama J, Tsunoda H, Sakuragawa N: Characterization of human amniotic epithelial cells transformed with origin-defective SV40 T-antigen gene. Tohoku J Exp Med 182:75-82, 1997
49. Tsubura A, Morii S, Ueda S, et al: Immunohistochemical demonstration of MAM-3 and MAM-6 antigens in normal human skin appendages and their tumors. Arch Dermatol Res 279:550-557, 1987
50. Van Muijen GNP, Ruiter DJ, Franke WW, Achtstätter T, Haasnoot WHB, Ponec M, Warnaar SO: Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res 162:97-113, 1986
51. Wang HC, Fedoroff S: Banding in human chromosomes treated with trypsin. Nature New Biol 235:52-54, 1972
52. Wang Y-C, Neckelmann N, Mayne A, Herskowitz A, Srinivasan A, Sell KW, Ahmed-Ansari A: Establishment of a human fetal cardiac myocyte cell line. In Vitro Cell Dev Biol 27A:63-74, 1991
53. Xia L, Zouboulis Ch, Detmar M, Mayer-da-Silva A, Stadler R, Orfanos CE: Isolation of human sebaceous glands and cultivation of sebaceous gland-derived cells as an in vitro model. J Invest Dermatol 93:315-321, 1989
54. Zouboulis ChC: Sebaceous cells in monolayer culture. In Vitro Cell Dev Biol 28A:699, 1992
55. Zouboulis ChC, Korge B, Akamatsu H, Xia L, Schiller S, Gollnick H, Orfanos CE: Effects of 13-cis-retinoic acid, all-trans-retinoic acid and acitretin on the proliferation, lipid synthesis and keratin expression of cultured human sebocytes in vitro. J Invest Dermatol 96:792-797, 1991a
56. Zouboulis ChC, Garbe C, Krasagakis K, Krüger S, Schröder K, Orfanos CE: A fluorometric rapid microassay to identify anti-proliferative compounds for human melanoma cells in vitro. Melanoma Res 1:91-95, 1991b
57. Zouboulis ChC, Xia L, Detmar M, Bogdanoff B, Giannakopoulos G, Gollnick H, Orfanos CE: Culture of human sebocytes and markers of sebocytic differentiation in vitro. Skin Pharmacol 4:74-83, 1991c
58. Zouboulis ChC, Korge BP, Mischke D, Orfanos CE: Altered proliferation, synthetic activity, and differentiation of cultured human sebocytes in the absence of vitamin A and their modulation by synthetic retinoids. J Invest Dermatol 101:628-633, 1993
59. Zouboulis ChC, Krieter A, Gollnick H, Orfanos CE: Progressive differentiation of human sebocytes in vitro is characterized by increased cell size and altered antigcnic expression and is regulated by culture duration and retinoids. Exp Dermatol 3:151-160, 1994
60. Zouboulis ChC, Xia L, Akamatsu H, et al: The human sebocyte culture model provides new insights into development and management of seborrhoea and acne. Dermatology 196:21-31, 1998