Bromodeoxyuridine labelling as an alternative method to identify 6- thioguanine-resistant mutant lymphocytes in humans

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 431, Issue 2, 1999, Pages 223-231

  Maffei, Francesca ^a   Vigagni, Fernanda ^a   Norppa, Hannu ^b   Hrelia, Patrizia ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b FINNISH INSTITUTE OF OCCUPATIONAL HEALTH   (Finland)

Author keywords

6 Thioguanine resistance; Bromodeoxyuridine; HPRT; Human lymphocyte; Mutation

Indexed keywords

TIOGUANINE;

ANTIBODY LABELING; ARTICLE; AUTOANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE MUTATION; GENOTOXICITY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE TEST; LYMPHOCYTE CLONE; PRIORITY JOURNAL;

BROMODEOXYURIDINE; CELL CULTURE TECHNIQUES; CELLS, CULTURED; FLUORESCEIN; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; GENETIC TECHNIQUES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LYMPHOCYTES; MUTATION; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE; THIOGUANINE;

EID: 0033396677     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(99)00165-7     Document Type: Article

References (21)

1. Cole J., Skopek T.R. Somatic mutant frequency, mutation rates and mutational spectra in the human population in vivo. Mutat. Res. 304:1994;33-105.
2. Strauss G.H., Albertini R.J. Enumerations of 6-thioguanine-resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivo. Mutat. Res. 61:1979;353-379.
3. Ostrosky-Wegman P., Montero M.R., Cortinas de Nava C., Tice R.R., Albertini R. The use of bromodeoxyuridine labelling in the human lymphocytes HGPRT somatic mutation assay. Mutat. Res. 231:1987;49-56.
4. Montero R., Norppa H., Autio K., Lindholm C., Ostrosky-Wegman P., Sorsa M. Determination of 6-thiguanine-resistant lymphocytes in human blood by immunohistochemical anti-bromodeoxyuridine staining. Mutagenesis. 6:1991;169-170.
5. Hageman G., Welle I., Stierum R., Albering H., Kleinjans J. Detection of 6-thioguanine-resistant human peripheral blood lymphocytes using 5-bromodeoxyuridine labelling in combination with immunocytochemical staining. Mutagenesis. 8:1993;495-501.
6. Albertini R.J., Sylwester D.L. 6-Thioguanine-resistant lymphocytes in human blood. Kilbey B.J., Legator M.S., Nichols W., Ramel C. Handbook of Mutagenicity Test Procedures. 2nd edn. 1984;357-372 Elsevier, New York.
7. Everson R.B., Griffin F.M., Capizzi R.L. Use of phytohemagglutinin response to determine the extent that somatic cell mutation accounts for 6-thioguanine resistance of human blood mononuclear cell. Mutat. Res. 147:1985;369-377.
8. Gocke E., Eckhardt K., King M.T., Wild D. Autoradiographic detection of 6-thioguanine-resistant lymphocytes of mice. A novel system in somatic mutagenesis testing. Mutat. Res. 113:1983;455-465.
9. Vigagni F., Hrelia P., Cantelli-Forti G., Sorsa M., Norppa H. An improved assay to detected 6-thioguanine-resistant mutants in human lymphocytes. J. Exp. Clin. Cancer Res. 14:1995;171-173.
10. Albertini R.J., Nicklas J.A., Fuscoe J.C., Skopek T.R., Branda R.F., O'Neil J.P. In vivo mutations in human blood cells: biomarkers for molecular epidemiology. Environ. Health Perspect. 99:1993;135-141.
11. Stierum R.H., Hageman G.J., Welle I.J., Albering H.J., Schreurs J.G., Kleinjans J.C. Evaluation of exposure reducing measures on parameters of genetic risk in a population occupationally exposed to coal fly ash. Mutat. Res. 319:1993;245-255.
12. van Maanen J.M., Welle I.J., Hageman G., Dallinga J.W., Mertens P.L., Kleinjans J.C. Nitrate contamination of drinking water: relationship with HPRT variant frequency in lymphocyte DNA and urinary excretion of N-nitrosamines. Environ. Health Perspect. 104:1996;522-528.
13. Morley A.A., Cox S., Wigmore D., Seshadri R., Dempsey J.L. Enumeration of thioguanine-resistant lymphocytes using autoradiography. Mutat. Res. 95:1982;363-375.
14. Johannisson A., Eriksson B., Amneus H., Zetterberg G. Attempts to use the HPRT assay as an automated short-term monitor for an acute exposure to mutagens. Cell Biol. Toxicol. 8:1992;233-253.
15. Ammenheuser M.M., Hastings D.A., Whorton E.B., Ward J.B. Frequencies of HPRT mutant lymphocytes in smokers, non-smokers, and former smokers. Environ. Mol. Mutagen. 30:1997;131-138.
16. Albertini R.J., Sullivan L.M., Berman J.K., Greene L.J., Stewart J.A., Silveira J.M., O'Neill J.P. Mutagenicity monitoring in humans by autoradiographic assay for mutant T-lymphocytes. Mutat. Res. 204:1988;481-492.
17. Albertini R.J. Studies with T-lymphocytes: an approach to human mutagenicity monitoring. Bridges B.A., Butterworth B.E., Weinstein I.B. Indicators of Genotoxic Exposure, Banbury Report 13. 1982;393-412 Cold Spring Harbor Laboratory, Cold Spring Harbor.
18. Albertini R.J., Castle K., Borcherdinng W.R. T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood. Proc. Natl. Acad. Sci. U.S.A. 79:1982;6617-6621.
19. Strauss G.H.S. Non random cell killing in cryopreservation: implication for performance of the battery of leukocytes tests (BLT): I. Toxic and immunotoxic effects. Mutat. Res. 252:1991;1-15.
20. Albertini R.J., Allen E.F., Quinn A.S., Albertini M.R. Human somatic cell mutation: in vivo variant lymphocyte frequencies as determined by 6-thioguanine resistance. Hook E.B., Porter I.H. Population and Biological Aspects of Human Mutation: Birth Defects Institute Symposium XI. 1981;235-263 Academic Press, New York.
21. Podlutsky A., Bastlova T., Lambert B. Reduced proliferation rate of hypoxanthinephosphoribosyl transferase mutant human T-lymphocytes in vitro. Environ. Mol. Mutagen. 28:1996;13-18.