Subtractive hybridization and differential screening identified two genes differentially expressed after induction of in vitro (atypical) terminal differentiation in the NSCLC-N6 cell line by a marine substance (Bistramide K)

Anticancer Research

Volumn 19, Issue 6 B, 1999, Pages 5361-5365

  Siavoshian, S ^a   Jacquot, C ^a   Biard, J F ^a   Briand, G ^a   Roussakis, C ^a  

^a UNIVERSITÉ DE NANTES   (France)

Author keywords

cDNA sequencing; Differential hybridization; NSCLC; Ribosomal protein L35A; Subtractive cDNA library; Terminal differentiation

Indexed keywords

ANTINEOPLASTIC AGENT; BISTRAMIDE K; DNA; MESSENGER RNA; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; CELL CYCLE G1 PHASE; CELL DIFFERENTIATION; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA LIBRARY; DNA SEQUENCE; GENE INDUCTION; GENE ISOLATION; HUMAN; HUMAN CELL; LUNG NON SMALL CELL CANCER; MITOSIS INHIBITION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ANTINEOPLASTIC AGENTS; BASE SEQUENCE; CARCINOMA, NON-SMALL-CELL LUNG; CELL DIFFERENTIATION; DNA, COMPLEMENTARY; ETHERS, CYCLIC; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; LUNG NEOPLASMS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; RNA, MESSENGER; SUBTRACTION TECHNIQUE; TUMOR CELLS, CULTURED;

EID: 0033391235     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Boring CC, Squires TS, Tong TT, Montgomery S: Cancer Statistics, 1994. CA Cancer J Clin 44: 7-26, 1994.
2. Ginsberg RJ, Kris MG, Armstrong JG: Non-Small-Cell lung cancer. In: Cancer Principal & Practice of Oncology. 4th Edn. (DeVita Jr VT, Hellman S, Rosenberg S, eds). Philadelphia, J.B. Lippincott Company, 1994, 673.
3. Boffetta P, La Vecchia C, Levi F, Lucchini F: Mortality patterns and trends for lung cancer and other tobacco-related cancers in the Americas 1955-1989. Int J Epidemiol 22: 377-384, 1993.
4. Ihde DC, Pass HI, Glatstein EJ: Small cell lung cancer. In: Cancer Principal & Practice of Oncology (DeVita Jr, VT, Hellman S, Rosenberg S, eds). Philadelphia, J.B. Lippincott Company, 1993, 723-758.
5. Ginsberg RJ, Kris MG, Armstrong JG: Non-Small-Cell lung cancer. In: Cancer Principal & Practice of Oncology. (DeVita Jr. VT, Hellman S, Rosenberg S, eds). Philadelphia, J.B. Lippincott Company, 1993, 673-723.
6. Walling J: Chemotherapy of advanced non-small-cell lung cancer. Respiratory Medicine 8: 649-657, 1994.
7. Hanauske AR: The development of new chemotherapeutic agents. Anti-Cancer Drugs 7 (suppl 2): 29-32, 1996.
8. Riou D, Roussakis C, Biard JF, Verbist JF: Comparative study of antitumor activity of bistramide A, D and K against a non-small-cell bronchopulmonary carcinoma. Anticancer Res 13: 2331-2334, 1993.
9. 30-latex and PCR: Identification of cellular genes which are overexpressed in senescent human diploid flbroblasts. Analytical Biochemistry 214: 58-64, 1993.
10. Roussakis C, Gratas C, Audoin AF, Le Boterff J, Dabouis C, Andre MJ, Moyon E, Vo NH, Verbist JF: Study in vitro drug sensivity on a newly established cell line from a primary bronchial epidermoid carcinoma and human origin (NSCLCN6). Anticancer Res 11: 2239-2244, 1991.
11. Chomczynski P and Sacchi N: Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159, 1987.
12. Sasaki YF, Ayusawa D, Oishi M: Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system. Nucleic Acids Research 22: 987-992, 1994.
13. 30-latex for rapid purification of poly(A)+ mRNA and for hybrid subtraction with in situ reverse transcribed cDNA. Biochimica-Biophysica-Acta 1156: 204-212, 1993.
14. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467, 1977.
15. Ulbrich N, Wool IG, Ackerman E, Sigler PB: The identification by affinity chromatography of the rat liver ribosomal proteins that bind elongator and initiator transfer ribonucleic acids. The Journal of Biology Chemistry 255: 7010-7016, 1980.
16. Tanaka T, Wakasugi K, Kuwano Y, Ishikawa K, Ogata K: Nucleotide sequence of cloned cDNA specific for rat ribosomal protein L35a. Eur J Biochem 154: 523-527, 1986.
17. Amaldi F, Beccari E, Bozzoni I, Luo ZX, Pierandrei-Amaldi P: Nucleotide sequences of cloned cDNA fragments specific for six Xenopus laevis ribosomal proteins. Gene 17: 311-316, 1982.
18. Colombo P, Read M, Fried M: The human L35a ribosomal protein (RPL35A) gene is located at chromosome band 3q29-qter. Genomics 32: 148-150, 1996.
19. Wool IG: Extraribosomal functions of ribosomal proteins. Trends Biochem Sci 21: 164-165, 1996.
20. Mafune KI, Wong JM, Staniunas RJ, Lu ML, Ravikumar TS, Chen LB, Steele GD: Ubiquitin hybrid protein gene expression during human colon cancer progression. Arch Surg 126: 462-466, 1991.