Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development

Plant Molecular Biology

Volumn 41, Issue 6, 1999, Pages 837-849

  Zou, Jitao ^a   Qi, Qungang ^a   Katavic, Vesna ^b   Marillia, Elizabeth F ^a   Taylor, David C ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b Saskatchewan Wheat Pool   (Canada)

Author keywords

pyruvate dehydrogenase (lipoamide) kinase (EC 2.7.1.99); Arabidopsis thaliana; Early flowering; Mitochondria; Plant development; Pyruvate dehydrogenase complex; Seed oil

Indexed keywords

AMINO ACID SEQUENCE; ANTISENSE DNA; CELL CYCLE; CELL RESPIRATION; COMPLEMENTARY DNA; ENZYME ACTIVITY; ENZYME SUBUNIT; GENE REPRESSION; MOLECULAR CLONING; MONOCLONAL ANTIBODY; PLANT DEVELOPMENT; PLANT GROWTH; PYRUVATE DEHYDROGENASE KINASE; TRANSGENIC PLANT; WESTERN BLOTTING; WILD RELATIVE;

ARABIDOPSIS; CLONING, MOLECULAR; DNA, ANTISENSE; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; HUMANS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; PHENOTYPE; PHOSPHORYLATION; PLANTS, GENETICALLY MODIFIED; PROTEIN KINASES; PYRUVATE DEHYDROGENASE COMPLEX; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

ARABIDOPSIS THALIANA; ESCHERICHIA COLI; MAMMALIA;

EID: 0033390795     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006393726018     Document Type: Article

References (47)

1. ap Rees, T., Bryce, J.H., Wilson, P.M. and Green, J.H. 1983. Role and location of NAD malic enzyme in thermogenic tissue of Araceae. Arch Biochem Biophys 227: 511-521.
2. Bechtold, N., Ellis. J. and Pelletier, G. 1993. In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C.R. Acad. Sci. Ser. III Sci. Vie. 316: 1194-1199.
3. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Budde, R.J.A. and Randall, D.D. 1990. Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner. Proc. Natl. Acad. Sci. USA 87: 673-676.
5. Budde, R.J.A., Fang, T.K. and Randall, D.D. 1988. Regulation of the phosphorylation of mitochondrial pyruvate dehydrogenase complex in situ. Plant Physiol. 88: 1031-1036.
6. Coleman, J. 1990. Characterization of Escherichia coli cells deficient in 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. J. Biol. Chem. 256: 17215-17221.
7. Coleman, J. 1992. Characterization of the Escherichia coli gene for 1-acyl-sn-glycerol-3-phosphate acyltransferase (plsC). Mol. Gen. Genet. 232: 295-303.
8. Datla, R.S.S., Hammerlindl, J.K., Panchuk, B., Pelcher, L.E. and Keller, W.A. 1992. Modified binary plant transformation vectors with the wild-type gene encoding NPTII. Gene 211: 383-384.
9. Datla, R.S.S., Bekkaoui, R, Hammerlindl, J., Pilate, G., Dunstan, D.I. and Crosby, W.L. 1993. Improved high-level constitutive foreign gene expression in plants using an AMV RNA4 untranslated leader sequence. Plant Sci. 94: 139-149.
10. Elledge, S.J., Mulligan, J.T., Ramer, S.W., Spottswood, M. and Davis, R.W. 1991. λYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735.
11. Grof, C.P.L., Winning, B.M., Scaysbrook, T.P., Hill, S.A. and Leaver, C.J. 1995. Mitochondrial pyruvate dehydrogenase: molecular cloning of the E1α subunit and expression analysis. Plant Physiol. 108: 1623-1629.
12. Guan, Y., Rawsthorne, S., Scofield, G., Shaw, P. and Doonan, J. 1995. Cloning and characterization of a dihydrolipoamide acetyltransferase (E2) subunit of the pyruvate dehydrogenase complex from Arabidopsis thaliana. J. Biol. Chem. 270: 5412-5417.
13. Havelange, A. 1980. The quantitative ultrastructure of the meristematic cells of Xanthium strumarium during the transition to flowering. Am. J. Bot. 67: 1171-1178.
14. Hill, R.L. and Bradshaw, R.A. 1969. Fumarase. Meth. Enzymol. 13: 91-99.
15. Hill, S.A., Grof, C.P.L., Bryce, J.H. and Leaver, C.J. 1992. Regulation of mitochondrial function and biogenesis in cucumber (Cucumis sativus L.) cotyledons during early seedling growth. Plant Physiol. 99: 60-66.
16. Journet, E.P. and Douce, R. 1985. Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism. Plant Physiol. 79: 458-467.
17. Katavic, V., Haughn, G.W., Reed, D., Martin, M. and Kunst, L. 1994. In planta transformation of Arabidopsis thaliana. Mol. Gen. Genet. 245: 363-370.
18. Katavic, V., Reed, D.W., Taylor, D.C., Giblin, E.M., Barton, D.L., Zou, J.-T., MacKenzie, S.L., Covello, P.S. and Kunst, L. 1995. Alteration of fatty acid composition by an EMS-induced mutation in Arabidopsis thaliana affecting diacylglycerol acyltransferase activity. Plant Physiol 108: 399-409.
19. Knowles, V.L., McHugh, S.G., Hu, Z., Dennis, D.T., Miki, B. and Plaxton, W.C. 1998. Altered growth of transgenic tobacco lacking leaf cytosolic pyruvate kinase. Plant Physiol. 116: 45-51.
20. Korotchkina, L.G. and Patel, M.S. 1995. Mutagenesis studies of the phosphorylation sites of recombinant human pyruvate dehydrogenase. J. Biol. Chem. 270: 14297-14304.
21. Korotchkina, L.G., Tucker, M.M., Thekkumkara, T.J., Madhusudhan, K.T., Pons, G., Kim, H. and Patel, M.S. 1995. Overexpression and characterization of human tetrameric pyruvate dehydrogenase and its individual subunits. Prot. Express. Purif. 6: 79-90.
22. Landschütze, V., Willmitzer, L. and Müller-Röber, B. 1995. Inhibition of flower formation by antisense repression of mitochondrial citrate synthase in transgenic potato plants leads to a specific disintegration of the ovary tissues of flowers. EMBO J. 14: 660-666.
23. Lernmark, U. and Gardeström, P. 1994. Distribution of pyruvate dehydrogenase complex activities between chloroplasts and mitochondria from leaves of different species. Plant Physiol. 106: 1633-1638.
24. Lindstrom, J.T. and Vodkin, L.O. 1991. A soybean cell wall protein is affected by seed colour genotype. Plant Cell 3: 561-571.
25. Liu, S., Baker, J.C. and Roche, T.E. 1995. Binding of the pyruvate dehydrogenase kinase to recombinant constructs containing the inner lipoyl domain of the dihydrolipoyl acetyltransferase component. J. Biol. Chem. 270: 793-800.
26. Luethy, M.H., Miernyk, J.A. and Randall, D.D. 1994. The nucleotide and deduced amino acid sequences of a cDNA encoding the E1 beta-subunit of the Arabidopsis thaliana mitochondrial pyruvate dehydrogenase complex. Biochim. Biophys. Acta. 1187: 95-98.
27. Luethy, M.H., Miernyk, J.A. and Randall, D.D. 1995. The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1α-subunit. Gene 164: 251-254.
28. MacDonald, E.D. and ap Rees, T. 1983. Enzymic properties of amyloplasts from suspension cultures of soybean. Biochim. Biophys. Acta 755: 81-89.
29. Parkinson, J.S. and Kofoid, E.C. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26: 71-112.
30. Pearson, W.R. and Lipman, D.J. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85: 2444-2448.
31. Popov, K.M., Zhao, Y., Shimomura, Y., Kuntz, M.J. and Harris, R.A. 1992. Branched-chain α-ketoacid dehydrogenase kinase: molecular cloning, expression and sequence similarity with histidine protein kinase. J. Biol. Chem. 267: 13127-13130.
32. Popov, K.M., Kedishvili, N.Y., Zhao, Y., Shimomura, Y., Crabb, D.W. and Harris, R.A. 1993. Primary structure of pyruvate dehydrogenase kinase establishes a new family of eukaryotic protein kinases. J. Biol. Chem. 268: 26602-26606.
33. Popov, K.M., Kedishvili, N.Y., Zhao, Y., Gudi, R. and Harris, R.A. 1994. Molecular cloning of the p45 subunit of pyruvate dehydrogenase kinase J. Biol. Chem. 269: 29720-29724.
34. Randall, D.D., Rubin, P.M. and Fenko, M. 1977. Plant pyruvate dehydrogenase complex purification, characterization and regulation by metabolites and phosphorylation. Biochim. Biophys. Acta 485: 336-349.
35. Randall, D.D., Williams, M. and Rapp, B.J. 1981. Phosphorylation-dephosphorylation of pyruvate dehydrogenase complex from pea leaf mitochondria. Arch. Biochem. Biophys. 207: 437-444.
36. Reid, E.E., Thompson, P., Lyttle, R. and Dennis, D.T. 1977. Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids. Plant Physiol. 59: 842-848.
37. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
38. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503-517.
39. Taylor, D.C., Magus, J.R., Bhella, R., Zou, J.-T., MacKenzie, S.L., Giblin, E.M., Pass, E.W. and Crosby, W.L. 1992. Biosynthesis of triacylglyerols in Brassica napus L. cv. Reston. Target: trierucin. In: S.L. MacKenzie and D.C. Taylor (Eds.), Seed Oils for the Future, AOCS Press, Champaign, IL, pp. 77-102.
40. Thelen, J.J., Muszynski, M.G., Miernyk, J.A. and Randall, D.D. 1998a. Molecular analysis of two pyruvate dehydrogenase kinases from maize. J. Biol. Chem. 273: 26618-26623.
41. Thelen, J.J., Miernyk, J.A. and Randall, D.D. 1998b. Nucleotide and deduced amino acid sequences of the pyruvate dehydrogenase kinase from Arabidopsis thaliana. Plant Physiol. 118: 1533.
42. Thelen, J.J., Miernyk, J.A. and Randall, D.D. 1999. Molecular cloning and expression analysis of the mitochondrial pyruvate dehydrogenase from maize. Plant Physiol. 119: 635-644.
43. Veeger, C., Der Vartanian, D.V. and Zeylemaker, W.P. 1969. Succinate dehydrogenase. Meth. Enzymol. 13: 81-90.
44. Winter, K., Foster, J.G., Edwards, G.E. and Holtum, J.A.M. 1982. Intracellular localization of enzymes of carbon metabolism in Mesembryanthemum crystallinum exhibiting C3 photosynthetic characteristics or performing crassulacean acid metabolism. Plant Physiol. 69: 300-307.
45. Zhang, H., Goodman, H.M. and Jansson, S. 1997. Antisense inhibition of photosystem I antenna protein Lhca4 in Arabidopsis thaliana. Plant Physiol. 115: 1525-1531.
46. Zou, J.-T. and Taylor, D.C. 1998. Plant pyruvate dehydrogenase kinase gene. International Patent Application PCT/CA98/00096; abstract published: 09/02/98; Priority date: 10/02/97.
47. Zou, J.-T., Katavic, V., Giblin, E.M., Barton, D.L., MacKenzie, S.L., Keller, W.A., Hu, X. and Taylor, D.C. 1997. Modification of seed oil content and acyl composition in Brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell 9: 909-923.