Do immunoassays differentially detect different acidity glycoforms of FSH?

Clinical Endocrinology

Volumn 51, Issue 6, 1999, Pages 681-686

  Oliver, Robert L ^a   Kane, John W ^b   Waite, Annelise ^b   Mulders, John W M ^c   Robertson, William R ^a,d  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b HOPE HOSPITAL   (United Kingdom)

^c NV ORGANON   (Netherlands)

^d JOHN RADCLIFFE HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FOLLITROPIN;

ACIDITY; ARTICLE; HORMONE DETERMINATION; IMMUNOASSAY; IMMUNOREACTIVITY; INTERMETHOD COMPARISON; MEASUREMENT; PRIORITY JOURNAL;

ANALYSIS OF VARIANCE; DOSE-RESPONSE RELATIONSHIP, DRUG; FLUOROIMMUNOASSAY; FOLLICLE STIMULATING HORMONE; GLYCOSYLATION; HUMANS; IMMUNOENZYME TECHNIQUES; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; RADIOIMMUNOASSAY; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 0033387706     PISSN: 03000664     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2265.1999.00823.x     Document Type: Article

References (20)

1. Abushoufa, R.A., Talbot, J.A., Rafferty, B., Kane, J.W. & Robertson, W.R. (1998) The development of a sialic acid specific lectin-immunoassay for the measurement of glycoforms of human chorionic gonadotrophin in serum. Journal of Endocrinology, 156 (Suppl.), P239.
2. Anobile, C.J., Talbot, J.A., McCann, S.J., Padmanabhan, V. & Robertson, W.R. (1998) Glycoform composition of serum gonadotrophins through the normal menstrual cycle and in the postmenopausal state. Molecular Human Reproduction, 4, 631-639.
3. Birken, S., Agosto, G., Amr, S., Nisula, B., Cole, L., Lewis, J. & Canfield, R. (1988) Characterization of antisera distinguishing carbohydrate structures in the beta-carboxyl-terminal region of human chorionic-gonadotropin. Endocrinology, 122, 2054-2063.
4. Burgon, P.G., Robertson, D.M., Stanton, P.G. & Hearn, M.T.W. (1993) Immunological activities of highly purified isoforms of human FSH correlate with in-vitro bioactivities. Journal of Endocrinologv, 139, 511-518.
5. Chappel, S.C. (1995) Heterogeneity of follicle-stimulating-hormone -control and physiological-function. Human Reproduction Update, 1, 479-487.
6. Chen, W.Y. & Bahl, O.P. (1992) Polyclonal antibodies against the polypeptide and carbohydrate epitopes of recombinant human choriogonadotropin beta-subunit. Molecular and Cellular Endocrinology, 86, 57-66.
7. Cole, A., Omrani, A., Cermik, D., Singh, R.O.B. & Mahoney, M.J. (1998) Hyperglycosylated hCG, a potential alternative to hCG in Down syndrome screening. Prenatal Diagnosis., 18, 926-933.
8. Jeffcoate, S.L. (1997) Standardization of human follicle-stimulating hormone for therapy and diagnosis: report of an international workshop, National Institute of Biological Standards and Control, UK. 21-22 March 1996. Clinical Endocrinology, 46, 527-529.
9. Lambert, A., Talbot, J.A., Anobile, C.J. & Robertson, W.R. (1998) Gonadotrophin heterogeneity and biopotency: implications for assisted reproduction. Molecular Human Reproduction, 4, 619-629.
10. Mitchell, R., Bauerfeld, C., Schaefer, F., Scharer, K. & Robertson, W.R. (1994) Less acidic forms of luteinizing-hormone are associated with lower testosterone secretion in men on hemodialysis-treatment. Clinical Endocrinology, 41, 65-73.
11. Mitchell, R., Hollis, S., Crowley, V., Mcloughlin, J., Peers, N. & Robertson, W.R. (1995) Immunometric assays of luteinizing-hormone (LH) - differences in recognition of plasma-LH by anti-intact and beta-subunit-specific antibodies in various physiological and pathophysiological situations. Clinical Chemistry, 41, 1139-1145.
12. Mulders, J.W.M., Derksen, M., Swolfs, A. & Maris, F. (1997) Prediction of the in vivo biological activity of human recombinant follicle stimulating hormone using quantitative isoelectric focusing. Biologicals, 25, 269-281.
13. Oliver, R.L., Talbot, J.A., Kane, J., Mulders, J., Rafferty, B. & Robertson, W.R. (1998) Detection of charged FSH glycoforms with a sialic acid specific lectin assay. Journal of Endocrinology, 156(Suppl.), P241.
14. Robertson, W.R. & Mitchell, R. (1995) Bioassays and immunoassays: resolving the differences. Proceedings of UK NEQAS Meeting, 1, 1-11.
15. Robertson, W.R., Lambert, A., Mitchell, R. & Talbot, J.A. (1997) The interpretation of immuno-and bioassays of human FSH. In FSH Action and Intraovarian Regulation (eds. B.J.C.M. Fauser et al.), 33-50. Parthanon Press, Carnforth.
16. Simoni, M., Jockenhovel, F. & Nieschlag, E. (1993) Biological and immunological properties of the international standard for FSH-83/ 575 - isoelectrofocusing profile and comparison with other FSH preparations. Acta Endocrinologica, 128, 281-288.
17. Simoni, M., Jockenhovel, F. & Nieschlag, E. (1994) Polymorphism of human pituitary FSH - analysis of immunoreactivity and in-vitro bioactivity of different molecular-species. Journal of Endocrinology, 141, 359-367.
18. Stanton, P.G., Robertson, D.M., Burgon, P.G., Schmaukwhite, B. & Hearn, M.T.W. (1992) Isolation and physicochemical characterization of human follicle-stimulating-hormone isoforms. Endocrinology, 130, 2820-2832.
19. Storring, P.L. (1992) Assaying glycoprotein hormones - the influence of glycosylation on immunoreactivity. Trends in Biotechnology, 10, 427-432.
20. Ulloa-Aguirre, A., Midgley, A.R., Beitins, I.Z. & Padmanabhan, V. (1995) Follicle-stimulating isohormones - characterization and physiological relevance. Endocrine Reviews, 16, 765-787.