Detection of imipenem-resistant and metronidazole-resistant Bacteroides fragilis group strains in fecal samples

Clinical Microbiology and Infection

Volumn 5, Issue 12, 1999, Pages 753-758

  Fang, Hong ^a,b   Edlund, Charlotta ^a,b   Zhang, Guangming ^a   Hedberg, Maria ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b SÖDERTÖRN UNIVERSITY   (Sweden)

Author keywords

Bacteroides fragilis group; dirPCR; Imipenem; imsPCR; Metronidazole; purePCR; Resistance

Indexed keywords

AGAR; ANTIBIOTIC AGENT; BETA LACTAMASE; IMIPENEM; METRONIDAZOLE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTEROIDES FRAGILIS; CELL SEPARATION; CONTROLLED STUDY; DIARRHEA; DIFFUSION; FECES CULTURE; HUMAN; MAJOR CLINICAL STUDY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); BACTEROIDES FRAGILIS;

EID: 0033381050     PISSN: 1198743X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-0691.1999.tb00709.x     Document Type: Article

References (23)

1. Moore WEC, Holdeman LV. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol 1974; 27: 961-79.
2. Finegold SM. Anaerobic infections in humans: an overview. Anaerobe 1995; 1: 3-9.
3. Rasmussen BA, Bush K, Tally FP. Antimicrobial resistance in anaerobes. Clin Infect Dis 1997; 24(suppl 1): S110-20.
4. Turgeon P, Turgeon V, Gourdeau M, Duboid J, Lamothe F. Longitudinal study of susceptibilities of species of the Bacteroides fragilis group to five antimicrobial agents in three medical centers. Antimicrob Agents Chemother 1994; 38: 2276-9.
5. Bandoh-Tanaka K, Kato N, Watanabe K, Ueno K. Antibiotic susceptibility profiles of Bacteroides fragilis and Bacteroides thetaiotaomicron, in Japan from 1990 to 1992. Clin Infect Dis 1995; 20(suppl 2): S352-5.
6. Snydman DR, McDermott L, Cuchural GJ Jr, et al. Analysis of trends in antimicrobial resistance patterns among clinical isolates of Bacteroides fragilis group species from 1990 to 1994. Clin Infect Dis 1996; 23(suppl 1): S54-65.
7. Turner P, Edwards R, Weston V, Gazis A, Ispahani P, Greenwood D. Simultaneous resistance to metronidazole, co-amoxiclav, and imipenem in clinical isolates of Bacteroides fragilis. Lancet 1995; 345: 1275-7.
8. Ingham HR, Eaton S, Venables CW, Adams PC. Bacteroides fragilis resistant to metronidazole after long-term therapy. Lancet 1978; 1: 214.
9. Reysset G, Haggoud A, Sebald M. Genetics of resistance of Bacteroides species to 5-nitroimidazole. Clin Infect Dis 1993; 16(suppl 4): S401-3.
10. Hedberg M, Edlund C, Lindqvist L, Rylander M, Nord CE. Purification and characterization of an imipenem hydrolysing metallo-β-lactamase from Bacteroides fragilis. J Antimicrob Chemother 1992; 29: 105-13.
11. Rasmussen BA, Bush K. Carbapenem-hydrolyzing β-lactamases. Antimicrob Agents Chemother 1997; 41: 223-32.
12. Fang H, Hedberg M, Edlund C, Nord CE. Identification of the metallo-β-lactamase gene from clinical isolates of Bacteroides fragilis. Anaerobe 1999; 5: in press.
13. Kato N, Ou C, Kato H, et al. Detection of toxigenic Clostridium difficile in stool specimens by the polymerase chain reaction. J Infect Dis 1993; 167: 455-8.
14. Wilde J, Eiden J, Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol 1990; 28: 1300-7.
15. Pantosti A, Malpeli M, Wilks M, Menozzi MG, D'Ambrosio F. Detection of enterotoxigenic Bacteroides fragilis by PCR. J Clin Microbiol 1997; 35: 2482-6.
16. Zhang G, Weintraub A. Rapid and sensitive assay for detection of enterotoxigenic Bacteroides fragilis. J Clin Microbiol 1998; 36: 3545-8.
17. Reysset G. Genetics of 5-nitroimidazole resistance in Bacteroides species. Anaerobe 1996; 2: 59-69.
18. Trinh S, Reysset G. Detection by PCR of the nim genes encoding 5-nitroimidazole resistance in Bacteroides spp. J Clin Microbiol 1996; 34: 2078-84.
19. Summanen P, Baron EJ, Citron DM, Strong CA, Wexler HM, Finegold SM. Wadsworth anaerobic bacteriology manual, 5th edn. Belmont, Ca: Star Publishing Company, 1993: 119-22.
20. National Committee for Clinical Laboratory Standards. Interpretive categories and correlative minimum inhibitory concentrations (MICs), 4th edn. Document M11-A4. Villanova: NCCLS, 1997.
21. Greenwood D, Watt B, Duerden BI. Antibiotics and anaerobes. In Duerden BI, Draser BS, eds. Anaerobes in human disease. London: Edward Arnold, 1991: 415-29.
22. Nagy E, Földes J. Inactivation of metronidazole by Enterococcus faecalis. J Antimicrob Chemother 1991; 27: 63-70.
23. Nagy E, Werner H, Heizmann W. In vitro activity of daptomycin-metronidazole combinations against mixed bacterial cultures: reduced activity of metronidazole against Bacteroides species in the presence of Enterococcus faecalis. Eur J Clin Microbiol Infect Dis 1990; 9: 287-91.