Cloning and characterization of a gene (arpA) from Aspergillus oryzae encoding an actin-related protein required for normal nuclear distribution and morphology of conidiophores

Molecular and General Genetics

Volumn 262, Issue 4-5, 1999, Pages 758-767

  Hirozumi, K ^a   Nakajima, H ^a   Machida M , ^b   Yamaguchi M , ^c   Takeo K , ^c   Kitamoto K , ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATL INST BIOSCI HUM TECHNOL   (Japan)

^c CHIBA UNIVERSITY   (Japan)

Author keywords

Actin related protein; Aspergillus oryzae; Dynactin; Hyper branching hyphae; Nuclear migration

Indexed keywords

ACTIN; DYNACTIN;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS ORYZAE; CELL MIGRATION; CELL NUCLEUS; CONTROLLED STUDY; FUNGAL GENETICS; FUNGUS GROWTH; GENE CONSTRUCT; GENE ISOLATION; GENE MUTATION; GENE OVEREXPRESSION; INTRON; MOLECULAR CLONING; MYCELIUM; NEUROSPORA CRASSA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SEQUENCE HOMOLOGY;

ACTINS; AMINO ACID SEQUENCE; ASPERGILLUS ORYZAE; BASE SEQUENCE; CELL NUCLEUS; CLONING, MOLECULAR; DNA, FUNGAL; FUNGAL PROTEINS; GENES, FUNGAL; MICROSCOPY, ELECTRON, SCANNING; MOLECULAR SEQUENCE DATA; PLASMIDS;

ASPERGILLUS ORYZAE; FUNGI; MYXOGASTRIA; NEUROSPORA CRASSA;

EID: 0033376934     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380051138     Document Type: Article

References (42)

1. Borck J, Beggs JD, Brammer WJ, Hopkins AS, Murray NE (1976) The construction in vitro of transducing derivatives of phage lambda. Mol Gen Genet 146:199-207
2. Buxton FP, Gwynne DI, Da vies RW (1989) Cloning of a new bidirectionally selectable marker for Aspergillus strains. Gene 84:329-334
3. Casadaban MJ, Cohen SN (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179-207
4. Clark SW, Meyer DI (1992) Centractin is an actin homologue associated with the centrosome. Nature 359:246-250
5. Clark SW, Meyer DI (1994) ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle. J Cell Biol 127:129-138
6. Dutton JR, Johns S, Miller BL (1997) StuAp is a sequence-specific transcription factor that regulates developmental complexity in Aspergillus nidulans. EMBO J 16:5710-5721
7. Endow SA, Kang SJ, Satterwhite LL, Rose MD, Skeen VP (1994) Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J 13:2708-2713
8. Erba HP, Gunning P, Kedes L (1986) Nucleotide sequence of the human gamma cytoskeleton actin mRNA: anomalous evolution of vertebrate non-muscle actin genes. Nucleic Acids Res 14:5275-5294
9. Eshel D, Urrestarazu LA, Vissers S, Jauniauc J, van Vliet-Reedijk JC, Planta RJ, Gibbons IR (1993) Cytoplasmic dynein is required for normal nuclear segregation in yeast. Proc Natl Acad Sci USA 90:11172-11176
10. Fischer R, Timberlake WE (1995) Aspergillus nidulans apsA (anucleate primary sterigmata) encodes a coiled-coil protein required for nuclear positioning and completion of asexual development. J Cell Biol 128:485-498
11. Fyrberg C, Fyrberg E (1993) A Drosophila homologue of the Schizosaccharomyces pombe act2 gene. Biochem Genet 31:329-341
12. Gurr SJ, Unkles SE, Kinghorn JR (1987) The structure and organization of nuclear genes of filamentous fungi. In: Kinghorn JR (ed) Gene structure in eukaryotic microbes. IRL Press, Oxford, pp 93-139
13. Hara S, Kitamoto K, Gomi K (1992) New developments in fermented beverages and food with Aspergillus. In: Bennett JW, Klich MA (eds) Aspergillus: biology and industrial applications. Butterworth-Heinemann, Boston, pp 33-153
14. Harris SD, Morrell JL, Hamer JE (1994) Identification and characterization of Aspergillus nidulans mutants defective in cytokinesis. Genetics 136:517-532
15. Hata Y, Kitamoto K, Gomi K, Kumagai C, Tamura G (1992) Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylase. Curr Genet 22:85-91
16. Holmes KC, Popp D, Gebhard W, Kabsch W (1990) Atomic model of the actin filament. Nature 347:44-49
17. Holzbaur ELF, Vallee RB (1994) Dyneins: molecular structure and cellular function. Annu Rev Cell Biol 10:339-372
18. Kitamoto K, Kimura K, Gomi K, Kumagai C (1994) Electrophoretic karyotype and gene assignment to chromosomes of Aspergillus oryzae. Biosci Biotechnol Biochem 58:1467-1470
19. Lees-Miller JP, Henry G, Helfman DM (1992a) Identification of act2, an essential gene in the fission yeast Schizosaccharomyces pombe. Proc Natl Acad Sci USA 89:80-83
20. Lees-Miller JP, Helfman DM, Schroer TA (1992b) A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-246
21. Lye RJ, Porter ME, Scholey JM, McIntosh JR (1987) Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans. Cell 51:309-318
22. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
23. Minetoki T, Nunokawa K, Gomi K, Kitamoto K, Kumagai C, Tamura G (1996) Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding α-glycosidase. Curr Genet 30:432-438
24. Muhua L, Karpova TS, Cooper JA (1994) A yeast actin-related protein homologous to that in vertebrate dynactin complex is importart for spindle orientation and nuclear migration. Cell 78:669-679
25. Glued and a novel actin. J Biol Chem 268:15318-15323
26. Plamann M, Minke PF, Tinsley JH, Bruno KS (1994) Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi. J Cell Biol 127:139-149
27. Porter ME, Johnson KA (1989) Dynein structure and function. Annu Rev Cell Biol 5:119-151
28. Punt PJ, Veldhuisen G, van den Hondel CA (1994) Protein targeting and secretion in filamentous fungi. A progress report. Antonie Van Leeuwenhoek 65:211-216
29. Rasmussen CD, Means RL, Lu KP, May GS, Means AR (1990) Characterization and expression of the unique calmodulin gene of Aspergillus nidulans. J Biol Chem 265:13767-13775
30. Robb MJ, Wilson MA, Vierula PJ (1995) A fungal actin-related protein involved in nuclear migration. Mol Gen Genet 247:583-590
31. Schafer DA, Gill SR, Cooper JA, Heuser JE, Schroer TA (1994) Ultrastructural analysis of the dynactin complex: and actin- related protein is a component of a filament that resembles F-actin. J Cell Biol 126:403-412
32. Schroer TA, Sheetz MP (1991) Two activators of microtubule- based vesicle transport. J Cell Biol 115:1309-1318
33. Schroer TA, Bingham JB, Steven RG (1996) Actin-related protein 1 and cytoplasmic dynein-based motility - what's the connection? Trends Cell Biol 6:212-215
34. Suelmann R, Sievers N, Fischer R (1997) Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans. Mol Microbiol 25:757-769
35. Tada S, Gomi K, Kitamoto K, Takahashi K, Tamura G, Hara S (1991) Construction of a fusion gene comprising the Takaamylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae. Mol Gen Genet 229:301-306
36. Tsuchiya K, Tada S, Gomi K, Kitamoto K, Kumagai C, Jigami Y, Tamura G (1994) High level expression of the synthetic human lysozyme gene in Aspergillus oryzae. Appl Microbiol Biotechnol 38:109-114
37. Walker RA, Sheetz MP (1993) Cytoplasmic microtubule-associated motors. Annu Rev Biochem 62:429-451
38. Willins DA, Xiang X, Morris NR (1995) An alpha tubulin mutation suppresses nuclear migration mutations in Aspergillus nidulans. Genetics 141:1287-1298
39. Xiang X, Beckwith SM, Morris NR (1994) Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans. Proc Natl Acad Sci USA 91:2100-2104
40. Yamada O, Lee RB, Gomi K (1997) Transformation system for Aspergillus oryzae with double auxotrophic mutations, niaD and sC. Biosci Biotech Biochem 61:1367-1369
41. Yarden O, Plamann M, Ebbole DJ, Yanofsky C (1992) cot-1, a gene required for hyphal elongation in Neurospora crassa, encodes a protein kinase. EMBO J 11:2159-2166
42. Zhang Z, Tanaka Y, Nonaka S, Aizawa H, Kawasaki H, Nakata T, Horikawa N (1993) The primary structure of rat brain (cytoplasmic) dynein heavy chain, a cytoplasmic motor enzyme. Proc Natl Acad Sci USA 90:7928-7932