A simplified purification procedure of α-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography

Bioseparation

Volumn 8, Issue 1-5, 1999, Pages 153-158

  Noppe, Wim ^a   Haezebrouck, Petra ^a   Hanssens, Ignace ^a   De Cuyper, Marcel ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Expanded bed adsorption; Milk proteins; lactalbumin

Indexed keywords

ADSORPTION; ALPHA LACTALBUMIN; CALCIUM BINDING PROTEIN; CHROMATOGRAPHY; EXPANDED BED ADSORPTION CHROMATOGRAPHY; GEL; HYDROPHOBICITY; MILK; PRODUCT RECOVERY; PROTEIN PURIFICATION;

CALCIUM; CONFORMATIONS; DAIRY PRODUCTS; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; PROTEINS; PURIFICATION;

EXPANDED BED ADSORPTION; LACTALBUMIN;

ADSORPTION;

EID: 0033372298     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-94-017-1519-5_17     Document Type: Article

References (15)

1. Aschaffenburg R and Drewry J (1956) Improved method for the preparation of crystalline β-lactoglobulin and α-lactalbumin from cow's milk. Biochem. J. 65: 273-277.
2. Blomkalns AL and Gomez MR (1997) Purification of bovine α-lactalbumin by immobilized metal ion affinity chromatography. Prep. Biochem. & Biotechn. 27: 219-226
3. Conti A, Napolitano L, Lai P, Pinna W and Godovac-Zimmerman J (1989) Isolation of donkey whey proteins and N-terminal amino acid sequence of α-lactalbumins A and B ,β- lactoglobulins I and II and lysozyme. Milchwissenschaft 44: 138-141.
4. 2+ to bovine α-lactalbumin. Biochim. Biophys. Acta 912: 211-219.
5. Haezebrouck P, Noyelle K and Van Dael H (1998) Equilibrium and kinetic folding of pigeon lysozyme. Biochemistry 37: 6772-6780.
6. Holpert M and Cooper TG (1990) Improved assay of α-lactalbumin and galactosyltranferase. Anal. Biochem. 188: 168-175.
7. Lindahl L and Vogel HJ (1984) Metal-ion-dependent hydrophobic interaction chromatography of α-lactalbumins. Anal. Biochem. 140: 394-402.
8. Noppe W, Hanssens I and De Cuyper M (1996) Simple two-step procedure for the purification of highly active pure equine lysozyme. J. Chromatogr. 719: 327-331.
9. Quiles A (1994) Protein composition in milk of Spanish Murciano-Granadina goats as affected by days after kidding. Small Rum. Res. 14: 67-92.
10. Segawa T and Sugai S (1983) Interaction of divalent metal ions with bovine, human and goat α-lactalbumins. J. Biochem. 93: 1321-1328.
11. Shaw DC, Messer M, Scrivener AM, Nicholas KR and Griffiths M (1993) Isolation, partial characterization and amino acid sequence of α-lactalbumin from platypus (Ornithorhynchus anatinus) milk. Biochem. Biophys. Acta 1161: 177-186.
12. Splitt D, Mackenstedt I and Freitag R (1996) Preparative membrane adsorber chromatography for the isolation of cow milk components. J. Chromatogr. 729: 87-97.
13. Stevenson EM and Leaver J (1994) Chromatographic separation of the proteins of mouse milk. Int. Dairy Journal 4: 205-220.
14. 2+ binding to bovine α-lactalbumin J. Biol. Chem. 260: 10944-10947.
15. 2+ release. Biochemistry 35: 16753-16759.