Instability of phenotype and gene expression in long-term culture of carrot hairy root clones

Plant Cell Reports

Volumn 19, Issue 1, 1999, Pages 43-50

  Guivarc'h, A ^a   Boccara, M ^b   Prouteau, M ^a   Chriqui, D ^a  

^a SORBONNE UNIVERSITÉ   (France)

^b AGROPARISTECH   (France)

Author keywords

Glucuronidase; Agrobacterium rhizogenes; Daucus carota; Gene expression; Gus gene; NAM test; Opines; Root culture

Indexed keywords

CARROT; CELL CULTURE; CLONE; GENE EXPRESSION; HAIR ROOT; INOCULATION; PHENOTYPE; REPORTER GENE; SOUTHERN BLOTTING; TRANSGENE;

AGROBACTERIUM RHIZOGENES; DAUCUS CAROTA; EMBRYOPHYTA; EUKARYOTA; RHIZOBIUM;

EID: 0033371098     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990050708     Document Type: Article

References (52)

1. Aird ELH, Hamill J, Rhodes MJC (1988) Cytogenetic analysis of hairy root cultures from a number of plant species transformed by Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult 15:47-57
2. Amselem J, Tepfer M (1992) Molecular basis for novel root phenotypes induced by Agrobacterium rhizogenes A4 on cucumber. Plant Mol Biol 19:421-432
3. Banerjee-Chattopadhyay S, Schwemmin AM, Schwemmin DJ (1985) A study of karyotypes and their alterations in cultured and Agrobacterium transformed roots of Lycopersicon peruvianum Mill. Theor Appl Genet 71:258-262
4. Benjamin BD, Roja G, Heble MR (1993) Agrobacterium rhizogenes mediated transformation of Rauwolfia serpentina: regeneration and alkaloid synthesis. Plant Cell Tissue Organ Cult 35:253-257
5. Chilton MD, Tepfer DA, Petit A, David C, Casse-Delbart F, Tempé J (1982) Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295:432-434
6. Chriqui D, Guivarc'h A, Dewitte W, Prinsen E, Van Onckelen H (1996) Rol genes and root initiation and development. Plant Soil 187:47-55
7. David C, Tempé J (1993) Transformation in Catharanthus species (Madagascar Periwinkle). In: Bajaj YPS (eds) Biotechnology in agriculture and forestry, vol 22. Springer, Berlin Heidelberg New York, pp 144-155
8. David C, Chilton MD, Tempe J (1984) Conservation of T-DNA in plants regenerated from hairy-root cultures. Bio/Technology 2:73-76
9. Dehio C, Schell J (1994) Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible gene silencing. Proc Natl Acad Sci USA 91:5538-5542
10. Depicker AG, Jacobs AM, Van Montagu MC (1988) A negative selection scheme for tobacco protoplasts-derived cells expressing the T-DNA gene 2. Plant Cell Rep 7:63-66
11. De Vries-Uijtewaal E, Gilissen LW, Flipse E, Sree Ramulu K, De Groot B (1988) Characterization of root clones obtained after transformation of monohaploid and diploid potato genotypes with hairy root inducing strains of Agrobacterium. Plant Sci 58:193-202
12. Dewitte W, Prinsen E, Chriqui D, Tepfer M, Anselem J, Van Onckelen H (1990) Characterization of Cucumis sativus roots transformed with Agrobacterium rhizogenes. In: Int Symp Biochem Mechanisms Involved Growth Regul., Milan
13. Elmayan T, Vaucheret H (1996) Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally. Plant J 9:787-797
14. English JJ, Mueller E, Baulcombe DC (1996) Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes. Plant Cell 8:179-188
15. Flores HE, Curtis WR (1993) Approaches to understanding and manipulating the biosynthetic potential of plant roots. Ann N Y Acad Sci 665:188-209
16. Galbraith DW, Harkins KR, Madox JM, Ayres NM, Sharma P, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049-1051
17. Guerche P, Jouanin L, Tepfer D, Pelletier G (1987) Genetic transformation of oilseed rape (Brassica napus) by the Ri T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the transformed phenotype. Mol Gen Genet 206:382-386
18. Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987) New routes to secondary products. Bio/Technology 5:800-804
19. Hänisch ten Cate CH, Sree Ramulu K, Dijkhuis P, de Groot B (1987) Genetic stability of cultured hairy roots induced by Agrobacterium rhizogenes on tuber discs of potato cv 'Bintje'. Plant Sci 49:217-222
20. Hobbs SLA, Kpodar P, DeLong CMO (1990) The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15:851-864
21. Huffman GA, White FF, Gordon MP, Nester EW (1984) Hairy-root inducing plasmid: physical map and homology to tumor inducing plasmids. J Bacteriol 157:269-276
22. Jaziri M, Homes J, Shimomura K (1994) An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Plant Cell Rep 13:349-352
23. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901-3907
24. Jones JDG, Gilbert DE, Grady KL, Jorgensen RA (1987) T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives. Mol Gen Genet 207:478-485
25. Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep 5:239-242
26. Lipp Joao KH, Brown TA (1994) Long-term stability of root cultures of tomato transformed with Agrobacterium rhizogenes R1601. J Exp Bot 45:641-647
27. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
28. Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Agric Biol Chem 50:2715-2722
29. Marie D, Brown S (1993) A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biol Cell 78:47-51
30. Matzke MJ, Matzke AJM (1995) How and why do plants inactivate homologous (trans)genes ? Plant Physiol 107:679-685
31. McInnes E, Morgan AJ, Mulligan BJ, Davey MR (1991) Roots induced on cucumber cotyledons by the agropine Ri plasmid TR-DNA exhibit the transformed phenotype. Plant Cell Rep 9:647-650
32. Meins F, Kuncz C (1994) Silencing of chitinase expression in transgenic plants: an autoregulation model. In: Paszkowski J (ed) Homologous recombination and gene silencing in plants. Kluwer Scientific Publ, Dordrecht, The Netherlands, pp 335-348
33. Meyer P, Heidmann I, Niedenhof I (1993) Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic petunia. Plant J 4:89-100
34. Moore L, Warren G, Strobel G (1979) Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenes. Plasmid 2:617-626
35. Mugnier J, Mosse B (1987) Vesicular-arbuscular mycorrhizal infection in transformed root-inducing T-DNA roots grown axenically. Phytopathology 77:1045-1050
36. Mukundan U, Hjortso MA (1990) Thiophene content in normal and transformed root cultures of Tagetes erecta: a comparison with thiophene content in roots of intact plants. J Exp Bot 41:1497-1501
37. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissues culture. Physiol Plant 15:473-497
38. Nuutila AM, Toivonen L, Kauppinen V (1994) Bioreactor studies on hairy root cultures of Catharanthus roseus: comparison of three bioreactor types. Biotechnol Tech 8:61-66
39. Ooms G, Karp A, Burrell MM, Twell D, Roberts J (1985) Genetic modification of potato development usine Ri T-DNA. Theor Appl Genet 70:440-446
40. Ottaviani MP, Smits T, Hänisch ten Cate C (1993) Differential methylation and expression of the β-glucuronidase and neomycin phosphotransferase genes in transgenic plants of potato cv 'Bintje'. Plant Sci 88:73-81
41. Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Further extension of the opine concept: plasmid of Agrobacterium rhizogenes cooperates for opine degradation. Mol Gen Genet 190:204-210
42. Prinsen E, Bercetche J, Chriqui D, Van Onckelen H (1992) Pisum sativum epicotyls inoculated with Agrobacterium rhizogenes agropine strains harbouring various T-DNA fragments: morphology, histology and endogenous indole-3-acetic acid and indole-3-acetamide content. J Plant Physiol 140:75-83
43. Robaglia C, Vilaine F, Pautot V, Raimond F, Amselem J, Jouanin L, Casse-Delbart F, Tepfer M (1987) Expression vectors based on the Agrobacterium rhizogenes Ri plasmid transformation system. Biochimie 69:231-237
44. Tepfer D (1984) Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 57:959-967
45. Tepfer D (1989) Ri T-DNA from Agrobacterium rhizogenes: a source of genes having applications in rhizosphere biology and plant development, ecology and evolution. In: Kosuge T, Nester E (eds) Plant-microbe interactions, vol 3. McGraw Hill, New York, pp 294-342
46. Tepfer M, Casse-Delbart F (1987) Agrobacterium rhizogenes as a vector for transforming higher plants. Microbiol Sci 4:24-28
47. Tepfer D, Tempé J (1981) Production d'agropine par des racines formées sous l'action d' Agrobacterium rhizogenes. C R Acad Sci Ser C 292:153-156
48. Vaucheret H (1993) Identification of a general silencer for 19S and 35S promoters in a transgenic tobacco plant: 90 bp of homology in the promoter sequence are sufficient for trans-inactivation. C R Acad Sci Ser C 316:1471-1483
49. Vaucheret H (1994) Promoter-dependent trans-inactivation in transgenic tobacco plants: kinetic aspects of gene silencing and gene reactivation. C R Acad Sci Ser C 317:310-323
50. Vilaine F, Casse-Delbart F (1987) Independent induction of transformed roots by the TL and TR regions of the Ri plasmid of agropine type Agrobacterium rhizogenes. Mol Gen Genet 206:17-23
51. White FF, Taylor BH, Hufman GA, Gordon MP, Nester EW (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol 164:33-44
52. Yoshikawa T, Tsutomu F (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep 6:449-453