The mechanism of thrombin-induced prostacyclin synthesis in human endothelial cells with reference to the gene transcription of prostacyclin-related enzymes and Ca2+ kinetics

Journal of Pharmacological and Toxicological Methods

Volumn 41, Issue 4, 1999, Pages 173-182

  Sato, Toshiyuki ^a   Sawada, Shohei ^a   Tsuda, Yutaka ^a   Komatsu, Sumio ^a   Akamatsu, Naoaki ^a   Kono, Yoshihito ^a   Higaki, Tadashi ^a   Imamura, Hitoshi ^a   Tada, Yusuke ^a   Yamasaki, Seiki ^a   Tamagaki, Toshiyuki ^a   Nakagawa, Katsumi ^a   Tsuji, Hajime ^a   Nakagawa, Masao ^a  

^a KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

Author keywords

Ca2+; Competitive polymerase chain reaction; PGHS; PGI2; PLA2; Thrombin

Indexed keywords

CALCIUM ION; INOSITOL 1,4,5 TRISPHOSPHATE; PHOSPHOLIPASE A2; PROSTACYCLIN; PROSTAGLANDIN SYNTHASE; THROMBIN;

ARTICLE; CALCIUM HOMEOSTASIS; CALCIUM TRANSPORT; ENDOTHELIUM CELL; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROSTAGLANDIN SYNTHESIS; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; THROMBOCYTE AGGREGATION; VASCULAR ENDOTHELIUM;

CALCIUM; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; EPOPROSTENOL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; INOSITOL 1,4,5-TRISPHOSPHATE; PHOSPHOLIPASES A; POLYMERASE CHAIN REACTION; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES; RNA, MESSENGER; THROMBIN; TIME FACTORS; TRANSCRIPTION, GENETIC; UMBILICAL VEINS;

EID: 0033369610     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1056-8719(99)00039-8     Document Type: Article

References (32)

1. Carter A., Eisert W., Muller T. Thrombin stimulates inositol phosphate accumulation and prostacyclin synthesis in human endothelial cells from umbilical vein but not from omentum. Thromb Haemost. 61:1989;122-126.
2. Chomczynski P., Scchi N. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:1987;156-159.
3. DeWitt D. Prostaglandin endoperoxide synthase. regulation of enzyme expression Biochem Biophys Acta. 1083:1991;121-134.
4. Gilliland G., Perrin S., Blanchard K., Bunn H. Analysis of cytokine mRNA and DNA. detection and quantitation by competitive polymerase chain reaction Proc Natl Acad Sci USA. 87:1990;2725-2729.
5. 2+ indicators with greatly improved fluorescence properties. J Biol Chem. 260:1985;2725-2729.
6. Hallam T., Pearson J., Needham L. Thrombin-stimulated elevation of human endothelial-cell cytoplasmic free calcium concentration causes prostacyclin production. Biochem J. 251:1988;243-249.
7. Higuchi R., Krummel B., Saik R. A general method of in vitro preparation and specific mutagenesis of DNA fragments. study of protein and DNA interactions Nucleic Acids Res. 16:1988;7351-7367.
8. Hong S. Effect of bradykinin and thrombin on prostacyclin synthesis in endothelial cells from calf and pig aorta and human umbilical cord vein. Thromb Res. 18:1980;787-795.
9. Inoue H., Yokoyama C., Hara S., Tone Y., Tanabe T. Transcriptional regulation of human prostaglandin-endoperoxide synthase-2 gene by lipopolysaccharide and phorbol ester in vascular endothelial cells. J Biol Chem. 270 24965-24971:1995.
10. 2 mRNA in lung fibroblast and endothelial cells in culture. Biochem Biophys Res Commun. 197:1993;1465-1474.
11. Jaffe E., Grulich J., Weksler B., Hampel G., Watanabe K. Correlation between thrombin induced prostacyclin production and inositol trisphosphate and cytosolic free calcium levels in human endothelial cells. J Biol Chem. 262:1987;8557-8565.
12. Jaffe E., Nachman R., Becker C., Minick R. Culture of human endothelial cells derived from umbilical veins. J Clin Invest. 52:1973;2745-2756.
13. Jones D., Carlton D., McIntyre T., Zimmerman G., Prescott S. Molecular cloning of human prostaglandin endoperoxide synthase type II and demonstration of expression in response to cytokines. J Biol Chem. 268:1993;9049-9054.
14. 2+ kinetics and cyclic nucleotides in human vascular endothelial cells. J Kyoto Pref Univ Med. 102:1993;1017-1033.
15. Kosaka T., Miyata A., Ihara H., Har S., Sugimoto T., Takeda O., Takahashi E., Tanabe T. Characterization of the human gene (PTGS2) encoding prostaglandin-endoperoxide synthase 2. Eur J Biochem. 221:1994;889-897.
16. Kraemer S., Medae E., DeWitt D. Prostaglandin endoperoxide synthase gene structure; identification of the transcription start site and 5′-flanking regulatory sequences. Arch Biochem Biophys. 293:1992;391-400.
17. Kunkel L., Smith K., Boyer S., Borgaonkar D., Wachtel S., Miller O., Breg W., Jones H., Rary J. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc Natl Acad Sci USA. 74:1977;1245-1249.
18. Maier J., Hla T., Maciag T. Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells. J Biol Chem. 265:1990;10805-10808.
19. Maxam A., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci USA. 74:1977;560-564.
20. 2 synthesis in human umbilical vein endothelial cells. J Biol Chem. 268:1993;839-844.
21. 2 in mouse bone marrow-derived mast cells. J Biol Chem. 269:1994;22269-22275.
22. Saiki R., Gelfand D., Stoffel S., Scharf S., Higuchi R., Horn G., Mullis K., Erlich H. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:1988;487-491.
23. Sawada S., Toyoda T., Takamatsu H., Niwa I., Maebo N., Tsuji H., Nakagawa M., Ijichi H. Prostacyclin generation by cultured human vascular endothelial cells with reference to angiotensin I-converting enzyme. Jpn Circ J. 50:1986;242-247.
24. Smith W. Prostanoid biosynthesis and mechanisms of action. Am J Physiol. 263(suppl):1992;F181-F191.
25. 2 production by Ca antagonists. J Kyoto Pref Univ Med. 95:1986;205-222.
26. Vu T.-K.H., Wheaton V., Hung D., Coughlin S. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell. 64:1991;1057-1068.
27. Wang A., Doyl M., Mark D. Quantification of mRNA by the polymerase chain reaction. Proc Natl Acad Sci USA. 86:1989;9717-9721.
28. 2 production by cultured human umbilical vein endothelial cells. Cell Signal. 8:1996;247-251.
29. Weibel E., Palade E. New cytoplasmic components in arterial endothelia. Cell Biol. 23:1964;101-112.
30. Weksler B., Ley C., Jaffe E. Stimulation of endothelial production by thrombin, trypsin, and the ionophore A 23187. J Clin Invest. 62:1978;923-930.
31. Yoshida K., Nachmias V. Phorbol ester stimulates calcium sequestration in saponized human platelets. J Biol Chem. 262:1987;16048-16054.
32. Zyglewska T., Sanduja R., Ohash K., Loose-Mitchell D.S., Sanduja S.K., Wu K.K. Inhibition of endothelial cell prostaglandin H synthase gene expression by naproxen. Biochem Biophys Acta. 1131:1992;78-82.