Autometallographical localisation of Cu and Zn within target cell compartments of winkles following exposure to Cu and Zn mixtures

European Journal of Histochemistry

Volumn 43, Issue 4, 1999, Pages 323-334

  Soto, M ^a   Lekube, X ^a   Marigomez I ^a  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

Author keywords

Autometallography; Cu and Zn localisation; Metal mixtures; Protein bound metals

Indexed keywords

COPPER; PROTEIN; ZINC;

ANIMAL; ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; CYTOCHEMISTRY; DRUG EFFECT; DRUG POTENTIATION; METABOLISM; MOLLUSC; ORGAN SIZE; PROTEIN BINDING; SILVER STAINING; TISSUE DISTRIBUTION;

ANIMALS; COPPER; DRUG SYNERGISM; HISTOCYTOCHEMISTRY; MOLLUSCA; ORGAN SIZE; PROTEIN BINDING; PROTEINS; SILVER STAINING; SPECTROPHOTOMETRY, ATOMIC; TISSUE DISTRIBUTION; ZINC;

ANIMALIA; INVERTEBRATA; LITTORINA LITTOREA; MOLLUSCA;

EID: 0033369223     PISSN: 1121760X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Baron J.: In situ sites for xenobiotic activation and detoxification: implications for the differential susceptibility of cells to the toxic actions of environmental chemicals. In: Graumann W and Drukker J. (eds.) "Histo-and Cytochemistry as a tool in environmental toxicology. Progress in Histo- and Cytochemistry", Fischer Verlag, Stuttgart, pp. 32-44, 1991.
2. Brough C.N., and White K.N.: Localization of metals in the gastropod Littorina saxatilis (Prosobranchia: Littorinoidea) from a polluted site. Acta Zool. 71, 77-88, 1990.
3. Clark R.B.: Marine pollution. Clarendon Press, Oxford, 1992.
4. Coombs T.L.: The distribution of zinc in the oyster Ostrea edulis and its relation to enzymic activity and to other metals. Mar. Biol. 12, 170-178, 1972.
5. Danscher G.: Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electron microscopy. Histochemistry 71, 1-16, 1981.
6. Danscher G.: Autometallography. A new technique for light and electron microscopic visualization of metals in biological tissues (gold, silver, metal sulphides and metal selenides). Histochemistry 81, 331-335, 1984.
7. Depledge H.H., and Rainbow P.S.: Models of regulation and accumulation of trace metals in marine invertebrates. Comp. Biochem. Physiol. 97C, 1-7, 1990.
8. Fischer H.: Mytilus edulis as a quantitative indicator of dissolved Cd. Final study and synthesis. Mar. Ecol. Prog. Ser. 32, 265-278, 1986.
9. George S.G., Pirie B.J.S., Cheyne A.R., Coombs T.L., and Grant P.T.: Detoxification of metals by marine bivalves: an ultrastructural study of the compartmentation of copper and zinc in the oyster Ostrea edulis. Mar Biol. 45, 147-156, 1978.
10. Greim H.: General introduction into toxicology of environmental chemicals In: Graumann W and Drukker J. (eds.) "Histo-and Cytochemistry as a tool in environmental toxicology. Progress in Histo- and Cytochemistry", Fischer Verlag, Stuttgart, pp. 20-26, 1991.
11. Harrison F.L., and Berger R.: Effects of copper on the latency of lysosomal hexosaminidase in the digestive cells of Mytilus edulis. Mar. Biol. 68, 109-116, 1982.
12. Hemelraad J., and Herwig H.J.: Cadmium kinetics in fresh-water clams. IV. Histochemical localization of cadmium in Anodonta cygnea and Anodonta anatina exposed to cadmium chloride. Arch. Environ. Contam. Toxicol. 17, 337-343, 1988.
13. Hemelraad J., Holwerda D.A., and Zandee D.I.: Cadmium kinetics in freshwater clams. I. The pattern of cadmium accumulation in Anodonta cygnea. Arch. Environ. Contam. Toxicol. 15, 1-7, 1986.
14. Herwig H.J., Brands F., Krutwagen E., and Zandee D.I.: Bioaccumulation and histochemical localization of cadmium in Dreissena polymorpha exposed to cadmium chloride. Aquat. Toxicol. 15, 269-286, 1989.
15. Holwerda D.A.: Cadmium kinetics in freshwater clams. V. Cadmium-copper interaction in metal accumulation by Anodonta cygnea and characterization of the metal-binding protein. Arch. Environ. Contam. Toxicol. 21, 432-437, 1991.
16. Kraak M.H.S., Schoon H., Peelers W.H.M., and van Straalen N.M.: Chronic ecotoxicity of mixtures of Cu, Zn and Cd in the zebra mussel Dreissena polymorpha. Ecotox. Environ. Saf. 25, 315-327, 1993.
17. Lillie R.D.: Histopathologic technique and practical histochemistry. McGraw-Hill Inc., 1965.
18. Lovett-Doust J.L., Schmidt M., and Lovett-Doust L.L.: Biological assessment of aquatic pollution: a review, with emphasis on plants as biomonitors. Biol. Rev. 69, 147-186, 1994.
19. Lowe D.M., and Moore M.N.: The cytochemical distribution of zinc (ZnII) and iron (FeIII) in the common mussel, Mytilus edulis, and their relationship with lysosomes. J. Mar. Biol. Ass. UK. 59, 851-858, 1979.
20. Marigómez J.A., Cajaraville M.P., and Angulo E.: Cellular distribution of cadmium in the common winkle Littorina littorea (L.) determined by X-ray microprobe analysis and histochemistry. Histochemistry 94, 191-199, 1990a.
21. Marigómez J.A., and Ireland M.P.: Accumulation, distribution and loss of cadmium in the marine prosobranch Littorina littorea. Sci. Tot. Environ. 78, 1-12, 1989.
22. Marigómez J.A., and Ireland M.P.: A laboratory study of cadmium exposure in Littorina littorea in relation to environmental cadmium and exposure time. Sci. Tot. Environ. 90, 75-87, 1990.
23. Marigómez J.A., Ireland M.P., and Angulo E.: Correlation of cadmium shell-weight index with environmental stress indicators at the cellular and organismic levels in Littorina littorea. Mar. Ecol. Prog. Ser. 67, 171-176, 1990b.
24. Marigómez I., Soto M., Etxeberria M., and Angulo E.: Effects of size, sex, reproduction and trematode infestation on the quantitative structure of digestive tubules in stressed winkles. Zool. Jahr. Anat. 123, 319-336, 1993.
25. Marigómez I., Soto M., and Kortabitarte M.: Tissue-level biomarkers and biological effect of mercury on sentinel slugs, Arion ater. Arch. Environ. Contam. Toxicol. 57, 54-62, 1996.
26. Martoja R., and Martoja-Pierson, M.: Técnicas de Histología Animal. Toray Masson Ed. Barcelona, 1970.
27. Mason A.Z.: The uptake, accumulation and excretion of metals by the marine prosobranch gastropod mollusc Littorina littorea (L.). PhD. D. Thesis, Univ. Wales. 1983.
28. Mason A.Z., and Nott J.A.: A rapid, routine technique for the X-ray microanalysis of microincinerated cryosections: A SEM study of inorganic deposits in tissues of the marine gastropod Littorina littorea L. J. Histochem. Cytochem. 28, 1301-1311, 1980.
29. Mason A.Z., and Simkiss K.: Interactions between metals and their distribution in tissues of Littorina littorea (L.) collected from clean and polluted sites. J. Mar. Biol. Ass. 63, 661-672, 1983.
30. Mason A.Z, Simkiss K., and Ryan K.P.: The ultrastructural localization of metals in specimens of Littorina littorea collected from clean and polluted sites. J. Mar. Biol. Ass. 64, 699-720, 1984.
31. Nott J.A.: Cytology of pollutant metals in marine invertebrates: a review of microanalytical applications. Scan. Microsc. 5, 191-205, 1991.
32. Nott J.A, Bebbiano M.J., Langston W.J., and Ryan K.P.: Cadmium in the gastropod Littorina littorea. J. Mar Biol. Ass. 73, 655-665, 1993.
33. Pearse A.G.E.: Histochemistry. Theoretical and applied. Vol 1. Preparative and optical technology, 4th edn., Churchill Livingstone, London, 1980.
34. Pirie B.J.S., George S.G., Lytton D.G., and Thomson J.D.: Metal-containing blood cells of oysters: ultrastructure, histochemistry and X-ray microanalysis. J. Mar. Biol. Ass. 64, 115-123, 1984.
35. Robinson W.E., and Ryan D.K.: Metal interaction within the kidney, gill and digestive gland of the hard clam, Mercenaria mercenaria, following laboratory exposure to cadmium. Arch. Environ. Contam. Toxicol. 15, 23-30, 1986.
36. Simkiss K., and Mason A.Z.: Metal ions: metabolic and toxic effects. In: Wilbur K.M. (ed.) 'The Mollusca", Vol. 2, Environmental Biochemistry. Acad. Press Inc, New York, pp. 101-164, 1983.
37. Sokal R.R., and Rohlf F.J.: Biometría. Principios y métodos estadísticos en la investigación biológica. H. Blume Ed, Barcelona, 1979.
38. Soto M.: Simultaneous quantification of bioavailable metals in molluscs by means of cellular and tissue analysis: implications for monitoring metal pollution in water quality assessment. European PhD. Univ. Basque Country 1995.
39. Soto M., Kortabitarte M., and Marigómez I.: Bioavailable heavy metals in estuarine waters as assessed by metal/shell-weight indices in sentinel mussels, Mytilus galloprovincialis, Mar. Ecol. Prog. Ser. 125, 127-136, 1995.
40. Soto M., Cajaraville M.P., Angulo E., and Marigómez I.:. Autometallographic localization of protein-bound copper and zinc in the common winkle, Littorina littorea: a light microscopical study. Histochem. J. 28, 1-13, 1996a.
41. Soto M., Cajaraville M.P., and Marigómez I.: Tissue and cell distribution of copper, zinc and cadmium in the mussel Mytilus galloprovincialis determined by autometallography. Tissue and Cell. 28, 557-568, 1996b.
42. Soto M., Ireland M.P and Marigómez I.: The contribution of metal/shell weight index in target-tissues to metal body burden in sentinel marine molluscs. 1. Littorina littorea. Sci. Tot. Environ. 198, 135-147, 1997.
43. Soto M., and Marigómez I.: Techniques for the study of metals in cell biology. In: Cajaraville MP (ed.) Cell Biology in environmental toxicology. Univ. Basque Country Press Serv., Bilbo, pp. 59-88, 1995.
44. Soto M., Quincoces I., Lekube X., and Marigómez I.: Autometallographied metal content in digestive cells of winkles: a cost-effective screening tool to monitor Cu and Zn pollution. Aquat. Toxicol. 40, 123-140, 1998a.
45. Soto M., Quincoces I., and Marigómez I.: Autometallographical procedure for the localization of metal traces in molluscan tissues by light-microscopy. J. Histotechnol. 21, 123-127, 1998b.
46. Sprague J.B.: Toxicity and tissue concentrations of lead, zinc and cadmium for marine molluscs and crustacean. Int lead and zinc Res. Org, Inc. 215 pp., 1986.
47. Viarengo A.: Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level. CRC rev. 1, 295-317, 1989.