Production, characterization and purification of chitosanase from Bacillus cereus

Brazilian Journal of Chemical Engineering

Volumn 16, Issue 2, 1999, Pages 185-192

  Piza, F A T ^a   Siloto, A P ^a   Carvalho, C V ^a   Franco, T T ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIUM COMPOUNDS; ANIMAL CELL CULTURE; CHROMATOGRAPHY; ELECTROPHORESIS; HYDROLYSIS; IMPURITIES; MICROORGANISMS; PH EFFECTS; PHASE COMPOSITION; POLYSACCHARIDES; PROTEINS; PURIFICATION;

AERATION; AMMONIUM SULFATE; AQUEOUS TWO PHASE SYSTEM; BACILLUS CEREUS; CATION EXCHANGE CHROMATOGRAPHY; CHITOSANASE; FACTORIAL EXPERIMENTAL DESIGN;

ENZYMES;

EID: 0033359089     PISSN: 01046632     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0104-66321999000200011     Document Type: Article

References (11)

1. Boucher, I.; Dupuy, A.; Vidal, P.; Negebauer, W.A. and Brzezinski, R., Purification and characterization of a chitosanase from Streptomyces N174, Applied Microbiology Biotechnology, 38, 188 (1992).
2. Franco, T.T.; Andrews A. T. and Asenjo, J. A., Use of chemical modified proteins to study the effect of a single protein property on partitioning in aqueous two - phase systems, Biotechnology and Bioengineering, 49, 300 (1996).
3. Franco, T.T. and Kaul, R., Single step partitioning in aqueous two-phase systems, cap.6. In Methods in Biotechnology - Aqueous Two-Phase Systems: Methods And Protocols. Ed. Dr. Rajini Hatti-Kaul, Humana Press, New Jersey, USA Editor Series: Prof. John M. Walker - University Of Hertforshire, UK, 1998 (In press).
4. Haaland, P.D., Experimental Design in Biotechnology. Marcel Dekker, Inc., New York (1989).
5. Johansson, H.O., Karlstrom, T. F. and Haynes, C., J.Chromatogr.B 711:3-17 (1998).
6. Muzarelli. R.A.A., Biochemical significance of exogenous chitins and chitosans in animals and patients. Carbohyd. Polym., 20: 7-16 (1993).
7. Pelletier, A. and Sigush, J., Purification and characterization of three chitosanase activities from Bacillus megaterium P1, Applied Environmental Microbiology, 5, 844 (1990).
8. Sedmark, J. J. and Grossberg, S. E., A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G250, Analytical Biochemistry, 79, 544 (1977).
9. Somashekar, D. and Joseph, J., Chitosanases -Properties and Applications: A Review. In: "Bioresource Technology" Elsevier Science (1996).
10. Spiro, R.G., Analysis of sugars found in glycoproteins, Methods in Enzymology, 8, 7 (1966).
11. Uchida, Y. and Ohtakara, A., Chitosanase from Bacillus species, Methods in Enzymology, 161, 501 (1988).