Characterization of human insulin-like growth factor-binding proteins by two-dimensional polyacrylamide gel electrophoresis and western ligand blot analysis

Journal of Clinical Endocrinology and Metabolism

Volumn 84, Issue 5, 1999, Pages 1679-1684

  Weber, Matthias M ^a   Spöttl, Gerald ^a   Gössl, Christoph ^a   Engelhardt, Dieter ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SOMATOMEDIN BINDING PROTEIN;

ADULT; ARTICLE; AUTORADIOGRAPHY; DEGLYCOSYLATION; HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; WESTERN BLOTTING;

EID: 0033308077     PISSN: 0021972X     EISSN: None     Source Type: Journal    
DOI: 10.1210/jc.84.5.1679     Document Type: Article

References (20)

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5. The blind men and the elephant: A parable for the study of insulin-like growth factor binding proteins
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7. Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7
8. Analysis of serum insulin-like growth factor binding protein using Western blotting: Use of the method for titration of the binding proteins and competitive binding studies
9. Two-dimensional electrophoresis
10. The current state of two-dimensional electrophoresis with immobilized pH gradients
11. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyamide to nitrocellulose
12. Phosphorylation of insulin-like growth factor (IGF)-binding protein 1 in cell culture and in vivo: Effects on affinity for IGF-I
13. Two insulin-like growth factor (IGF)-binding proteins are responsible for the selective affinity for IGF-II of cerebrospinal fluid binding proteins
14. High resolution two-dimensional electrophoresis of proteins
15. Characterization of rat serum insulin-like growth-factor binding proteins by two-dimensional gel electrophoresis: Identification of a potentially novel form
16. Characterization of insulin-like growth factor binding proteins by two-dimensional polyacrylamide gel electrophoresis and ligand blot analysis
17. Characterization of an insulin-like growth factor binding protein (IGFBP-4) produced by the B104 rat neuronal cell line: Chemical and biological properties and differential synthesis by sublines
18. Methods to detect and analyze modified insulin-like growth factor binding proteins
19. Insulin-like growth factor binding proteins as growth inhibitors in children with chronic renal failure
20. Insulin-like growth factor binding protein-1: Recent findings and new directions