Development of scintillation-proximity assays for alpha adrenoceptors

Journal of Pharmacological and Toxicological Methods

Volumn 42, Issue 4, 1999, Pages 237-244

  Gobel, Jeff ^a   Saussy Jr , David L ^a   Goetz, Aaron S ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

Adrenoceptors; Filter binding; Scintillation proximity assay (SPA)

Indexed keywords

ALPHA ADRENERGIC RECEPTOR; LIGAND; MEMBRANE PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DRUG DETERMINATION; DRUG RECEPTOR BINDING; DRUG RESEARCH; DRUG SCREENING; FILTRATION; INCUBATION TIME; NONHUMAN; PROTEIN DETERMINATION; RAT; SCINTILLATION; STRUCTURE ACTIVITY RELATION;

ADRENERGIC ALPHA-AGONISTS; ADRENERGIC ALPHA-ANTAGONISTS; ANIMAL; BINDING, COMPETITIVE; CHO CELLS; FIBROBLASTS; HAMSTERS; RATS; RECEPTORS, ADRENERGIC, ALPHA; SCINTILLATION COUNTING;

EID: 0033303265     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1056-8719(00)00072-1     Document Type: Article

References (8)

1. A study of the adrenotropic receptors
2. Pharmacological characteristics of alpha-2 adrenergic receptors; comparison of pharmacologically defined subtypes with subtypes identified by molecular cloning
3. Relationship between the inhibition constant (K(i)) and the concentration of inhibitor which causes 50% inhibition of an enzymatic reaction
4. Structure activity relationships of a series of buspirone analogs at alpha-1 adrenoceptors; further evidence that rat aorta alpha-1 adrenoceptors are of the alpha-1D-subtype
5. Scintillation proximity radioimmunoassay utilizing 125I-labelled ligands