Study of lichens with different state of hydration by the combination of low temperature scanning electron and confocal laser scanning microscopies

International Microbiology

Volumn 2, Issue 4, 1999, Pages 251-257

  De Rfos, Asuncion Los ^a   Ascaso, Carmen ^b   Wierzchos, Jacek ^c  

^a CSIC   (Spain)

^b UNIVERSITY OF LLEIDA   (Spain)

^c UNIVERSITY OF GRAZ   (Austria)

Author keywords

Confocal laser scanning microscopy (clsm); Hydration desiccation; Lasallia hispanica; Low temperature scanning electron microscopy (ltsem); Pannelia omphalodes

Indexed keywords

WATER;

ARTICLE; CHEMISTRY; COLD; CONFOCAL MICROSCOPY; DESICCATION; LABORATORY DIAGNOSIS; LICHENS; METHODOLOGY; SCANNING ELECTRON MICROSCOPY; ULTRASTRUCTURE;

COLD; DESICCATION; LICHENS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, SCANNING; SPECIMEN HANDLING; WATER;

EID: 0033289731     PISSN: 11396709     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Ascaso C, Bro\vn DH, Rapsch S ( 1988) The effect of desiccation on pyrenoid structure in the oceanic lichen Parmelia laerigata. Lichenologist 20:31-39
2. Ascaso C, Galvan J (1976) The ultrastructure of the symbionts of Rliizocarpon gcograpliiciiin, Parmelia conspcrsa and Unibilicaria pustulala growing under dryness conditions. Protoplasma 87:409-418
3. Ascaso C, Valladares F, de los Rfos A (1995) New ultrastructural aspects of pyrenoids of thé lichen photobiont Trcbouxia (Microthamniales, Chloroph) ta). J Phycol 31:114-119
4. Bewley JD (1979) Physiological aspects of desiccation tolerance. Annu RevPhysiol30:195-238
5. Brown DH, Ascaso C, Rapsch S (1987) Ultrastructural changes in thé pyrenoid of the lichen Pannclia stitcata stored under controlled conditions. Protoplasma 136:136-144
6. Brown DH, Rapsch S, Beckett A, Ascaso C (1987) The effect of desiccation on cell shape in the lichen Parmelia sulcata Taylor. New Pliytol 105:295-299
7. Friedl T (1989) Comparative ultrastructure of pyrenoids in Trcbouxia (Microthamniales, Clorophyta). Plant Syst Evol 164:145-159
8. Honegger R (1995) Experimental studies with foliose macrolichens: fungal responses to spatial disturbance at the organismic level and to spatial problems at the cellular level during drought stress events. Can J Bot 73:3569-5578
9. Honegger R ( 1998) The lichen symbiosis-what is so spectacular about it?Lichenologist30:193-212
10. Honegger R, Peter M ( 1994) Routes of solute translocation and the location of water in hcteromerous lichens visualized with cryotechniques in light and electron microscopy. Symbiosis 16:167-186
11. Honegger R, Peler M, Scherrer S ( 1996) Drought-induced structural alterations at the mycobiont-photobiont interface in a range of foliose macrolichens. Protoplasma 190:221-232
12. Lange ÖL, Green TGA, Reichenberger H, Meyer A ( 1995) Photosynthetic depression at high thallus water contents in Lichens: concurrent use of gas exchange and fluorescence techniques with a cyanobacterial and a green algal Peltigera species. Bot Acta 109:43-50
13. Reynolds S (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:200-211
14. Scheidegger C (1994) Low-temperature scanning electron microscopy: the localization of free and perturbed water and its role in the morphology of the lichen symbionts. Crypt Bot 4:290-299
15. Scheidegger C, Schroeter B, Frey B (I995) Structural and functional processes during water vapour uptake and desiccation in selected lichens with green algal photobionts. Planta 197:399-409
16. Schroeter B, Scheidegger C (1995) Water relations in lichens at subzero temperatures: structural changes and carbon dioxide exchange in the lichen Umbilicaria aprina from continental Antarctica. New Phytol 131:273-285
17. Valladares F, Sancho LG, Ascaso C (1997) Water storage in the lichen family Umbilicareacea. Bot Acta 111:1-9