Factors affecting yield and survival of cells when suspensions are subjected to centrifugation: Influence of centrifugal acceleration, time of centrifugation, and length of the suspension column in quasi-homogeneous centrifugal fields

Cell Biochemistry and Biophysics

Volumn 31, Issue 3, 1999, Pages 231-245

  Katkov, Igor I ^a   Mazur, Peter ^b,c  

^a Semen and Embryo Preservation Lab   (United States)

^b OAK RIDGE NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Damage; Mechanical sensitivity; Recovery; Sedimentation

Indexed keywords

ANIMAL; ARTICLE; CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION; CYTOLOGY; MALE; MATHEMATICS; METHODOLOGY; MOUSE; SPERMATOZOON; THEORETICAL MODEL;

ANIMALS; CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION; MALE; MATHEMATICS; MICE; MODELS, THEORETICAL; SPERMATOZOA;

EID: 0033252199     PISSN: 10859195     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02738241     Document Type: Article

References (10)

1. Katkov, I. I. and Mazur, P. (1998) Influence of centrifugation regimes on motility, yield and cell associations of mouse spermatozoa. J. Androl. 19, 232-241.
2. Mortimer, D. (1994) Practical Laboratory Andrology. Oxford University Press, New York, NY.
3. Songsasen, N., Betteridge, K. J., and Leibo, S. P. (1997) Birth of live mice resulting from oocytes fertilized with cryopreserved spermatozoa. Biol. Reprod. 56, 143-152.
4. Lam, D. M., Furrer, R., and Bruce, W. R. (1970) The separation, physical characterization, and differentiation of spermatogonial cells of the mouse. Proc. Nat. Acad. Sci. USA 65, 192-199.
5. Meistrich, M. L., Bruce, W. R., and Clermont, Y. (1973) Cellular composition of fractions of mouse testis cells following velocity sedimentation separation. Exp. Cell. Res. 79, 213-227.
6. Wyrobek, A. J., Meistrich, M. L., Furrer, R., and Bruce, W. R. (1972) Physical characteristics of mouse sperm nuclei. Biophys. J. 16, 811-825.
7. Tanford, C. (1961) Frictional coefficient of solid spheres and ellipsoids, in Physical Chemistry of Macromolecules, Wiley & Sons Inc., New York, NY, pp. 326-328.
8. Miller, R. G. and Phillips, K. J. (1963) Separation of cells by velocity sedimentation. J. Cell Physiol. 73, 190-202.
9. Happel, J. and Brenner, H. (1965) The setting of orthothropic bodies, in Low Reionolds Numbers Hydrodynamics, Prentice Hall, Englewood Cliffs, NJ, pp. 220-223.
10. Cummings, J. M. and Woodall, P. F. (1985) On mammalian sperm dimension. J. Reprod. Fertil. 75, 153-175.