Application of YO-PRO-1 as an epifluorescent dye for in situ detection of small amount DNA in plant cells

Journal of Plant Research

Volumn 112, Issue 1, 1999, Pages 117-122

  Sodmergen, ^a   Niu, Jian Gong ^a   Li, Lin Jiang ^b   He, Jiang Xun ^c   Guo, Feng Li ^d  

^a PEKING UNIVERSITY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c Jiujiang Teachers College   (China)

^d CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Epifluorescence microscopy; Laser confocal microscopy; Organelle DNA; Plant cells; YO PRO 1

Indexed keywords

IPOMOEA; JASMINUM NUDIFLORUM; NICOTIANA TABACUM; TABACUM; VICIA FABA;

EID: 0033247082     PISSN: 09189440     EISSN: None     Source Type: Journal    
DOI: 10.1007/pl00013852     Document Type: Article

References (26)

1. Brakenhoff, G.J., Blom, P. and Barends, P. 1979. Confocal scanning light microscopy with high aperture immersion lenses. J. Microscopy 117: 219-232.
2. Casna, N.J. and Shub, D.A. 1982. Bacteriophage T4 as a generalized DNA-cloning vector. Gene 18: 297-309.
3. Coleman, A.W. 1978. Visualization of chloroplast DNA with the fluorochromes. Exp. Cell Res. 114: 95-100.
4. Inada, N., Sakai, A., Kuroiwa, H. and Kuroiwa, T. 1998. Three-dimensional analysis of the senescence program in rice (Oryza sativa L.) coleoptiles: investigations of tissues and cells by fluorescence microscopy. Planta 205: 153-164.
5. James, T.W. and Jope, C. 1978. Visualization by fluorescence of chloroplst DNA in higher plants by means of the DNA-specific probe 4'6-diamidino-2-phenylindole. J. Cell Biol. 79: 623-630.
6. Johansen, B., Seberg, O., Petersen, G. and Arctander, P. 1995. Does DAPI detect cytoplasmic DNA ? Amer. J. Bot. 82: 1215-1219.
7. Kuroiwa, T., Fujie, M. and Kuroiwa, H. 1992. Studies on the behavior of mitochondrial DNA: synthesis of mitochondrial DNA occurs actively in a specific region just above the quiescent center in the root meristem of Pelargonium zonale. J. Cell Sci. 101: 483-493.
8. Kuroiwa, T., Fujie, M., Mita, T. and Kuroiwa, H. 1991. Application of embedding of samples in Technovit 7100 resin to observations of small amounts of DNA in the cellular organelles associated with cytoplasmic inheritance. Appl. Fluoresc. Tech. 3: 23-25.
9. Kuroiwa, T., Kawazu, T., Uchida, H., Ohta, T. and Kuroiwa, H. 1993. Direct evidence of plastid DNA and mitochondrial DNA in sperm cells in relation to biparental inheritance of organelle DNA in Pelargonium zonale by fluorescence/electron microscopy. Eur. J. Cell Biol. 62: 307-313.
10. Kuroiwa, T. and Suzuki, T. 1980. An improved method for the demonstration of the in situ chloroplast nuclei in higher plants. Cell Struct. Funct. 5: 195-197.
11. Li, H.J. and Sodmergen. 1995. Maternal cytoplasmic inheritance and pollen nucleolytic activities in some Poaceae species. Cytologia 60: 173-181.
12. Miyamura, S., Kuroiwa, T. and Nagata, T. 1987. Disappearance of plastid and mitochondrial nucleoids during the formation of generative cells of higher plants revealed by fluorescence microscopy. Protoplasma 141: 149-159.
13. Nass, M.M.K. and Nass, S. 1963a. Intramitochondrial fibers with DNA characteristics. I. Fixation and electron staining reactions. J. Cell Biol. 19: 593-612.
14. Nass, S. and Nass, M.M.K. 1963b. Intramitochondrial fibers with DNA characteristics. II. Enzymatic and other hydrolytic treatments. J. Cell Biol. 19: 613-629.
15. Nemoto, Y., Kawano, S., Nakamura, S., Mita, T., Nagata, T. and Kuroiwa, T. 1988. Studies on plastid-nuclei (nucleoids) in Nicotiana tabacum L. I. Isolation of proplastid-nuclei from cultured cells and identification of proplastid-nuclear proteins. Plant Cell Physiol. 29: 167-177.
16. Satoh, M., Nemoto, Y., Kawano, S., Nagata, T., Hirokawa, H. and Kuroiwa, T. 1993. Organization of heterogeneous mitochondrial DNA molecules in mitochondrial nuclei of cultured tobacco cells. Protoplasma 175: 112-120.
17. Sodmergen, Bai, H.H., He, J.X., Kuroiwa, H., Kawano, S. and Kuroiwa, T. 1998. Potential for biparental cytoplasmic inheritance in Jasminum officinale and Jasminum nudiflorum. Sex. Plant Reprod. 11: 107-112.
18. Sodmergen, Chen, G.H., Hu, Z.M., Guo, F.L. and Guan, X.L. 1995. Male gametophyte development in Plumbago zeylanica: cytoplasm localization and cell determination in the early generative cell. Protoplasma 186: 79-86.
19. Sodmergen, Kawano, S., Tano, S. and Kuroiwa, T. 1989. Preferential digestion of chloroplast nuclei (nucleoids) during senescence of the coleoptile of Oryza sativa. Protoplasma 152: 65-68.
20. Sodmergen, Kawano, S., Tano, S. and Kuroiwa, T. 1991. Degradation of chloroplast DNA in second leaves of rice (Oryza sativa) before leaf yellowing. Protoplasma 160: 89-98.
21. Sodmergen, Kuroiwa, H., Nagata, N., Qian, Z.H., Liu, H., Guan, X.L. and Kuroiwa, T. 1997. Quantitative study of organelle nucleoids during the second pollen grain mitosis in Oenothera biennis. Protoplasma 198: 66-72.
22. Sodmergen, Luo, Y.Y., Kuroiwa, T. and Hu, S.Y. 1994. Cytoplasmic DNA apportionment and plastid differentiation during male gametophyte development in Pelargonium zonale. Sex. Plant Reprod. 7: 51-56.
23. Sodmergen, Suzuki, T., Kawano, S., Nakamura, S., Tano, S. and Kuroiwa, T. 1992. Behavior of organelle nuclei (nucleoids) in generative and vegetative cells during maturation of pollen in Lilium longiflorum and Pelargonium zonale. Protoplasma 168: 73-82.
24. Suzuki, T., Fujikura, K., Higashiyama, T. and Takata, K. 1997. DNA staining for fluorescence and confocal microscopy. J. Histochem. Cytochem. 45: 49-53.
25. Williamson, D.H. and Fennell, D.J. 1995. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. In D.M. Prescott, ed., Methods in Cell Physiology, vol 12, Academic Press, New York, pp. 335-351.
26. Wilson, T. and Sheppard, C. 1984. Theory and Practice of Scanning Optical Microscopy. Academic Press, San Diego.