Radiolabeling of receptor ligands by chemical incorporation of phosphorylation sites

Analytical Biochemistry

Volumn 274, Issue 1, 1999, Pages 104-109

  Inglese, J ^a   Glickman, J F ^a  

^a PHARMACOPEIA INC   (United States)

Author keywords

Cytokine; G protein coupled receptors; Phosite; Protein kinase A; Protein phosphorylation; Radioligand binding assays

Indexed keywords

AMIDES; ENZYMES; LIGANDS; MOLECULES; PEPTIDES; PHOSPHORYLATION; REAGENTS;

CYTOKINES; G PROTEIN COUPLED RECEPTORS; PHOSITE; PROTEIN KINASE A; PROTEIN PHOSPHORYLATION; RADIOLIGAND BINDING;

BIOSYNTHESIS;

ADENOSINE TRIPHOSPHATE; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYTOKINE; GUANINE NUCLEOTIDE BINDING PROTEIN; LEPTIN; N ACETYLCYSTEINYL[[SUCCINIMIDYL 6 (THIOACETYL)AMINO]HEXANOATE]SERYLARGINYLARGINYLALANYLSERYLVALYLTYROSINAMIDE; NEUROKININ A; PEPTIDE; PHOSPHORUS 32; RADIOLIGAND; REAGENT; RECEPTOR PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME SUBSTRATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; LIGAND BINDING; MASS SPECTROMETRY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RECEPTOR BINDING; SIGNAL TRANSDUCTION;

EID: 0033214833     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4256     Document Type: Article

References (20)

1. Lefkowitz R. J., Roth J., Pricer W., Pastan I. Science. 170:1970;633-635.
2. Cuatrecasas P. Proc. Natl. Acad. Sci. USA. 68:1971;1264-1268.
3. Bolton A. E., Hunter W. M. Biochem. J. 133:1973;529-538.
4. Hemmila I., Webb S. Drug Discovery Today. 2:1997;373-381.
5. Inglese J., Samama P., Patel S., Burbaum J., Stroke I. L., Appell K. Biochemistry. 37:1998;2372-2377.
6. Hart H. E., Greenwald E. B. Mol. Immunol. 16:1979;265-267.
7. Baum E. Z., Johnston S. H. Anal. Biochem. 237:1996;129-134.
8. Bosworth N., Towers P. Nature. 341:1989;167-168.
9. Li B. L., Langer J. A., Schwartz B., Petska S. Proc. Natl. Acad. Sci. USA. 86:1989;558-562.
10. Garland A. M., Grady E. F., Lovett M., Vigna S. R., Frucht M. M., Krause J. E., Bunnett N. W. Mol. Pharmacol. 49:1996;438-446.
11. Cheng Y.-C., Prusoff W. H. Biochem. Pharmacol. 22:1973;3099-3108.
12. Patacchini R., Maggi C. A. Arch. Int. Pharmacodyn. Ther. 329:1995;161-184.
13. Nicola N. A. Guidebook to Cytokines and Their Receptors. 1994;Oxford Univ. Press, New York.
14. Zhang F., Basinski M. B., Beals J. M., Briggs S. L., Churgay L. M., Clawson D. K., DiMarchi R. D., Furman T. C., Hale J. E., Hsuing H. M., Schoner B. E., Smith D. P., Zhang X. Y., Wery J. P., Schevitz R. W. Nature. 387:1997;206-209.
15. Burcher E., Buck S. Y., Lovenberg W., O'Donohue T. L. J. Pharmacol. Exp. Ther. 236:1986;819-831.
16. Tartaglia L. A., Dembski M., Weng X., Deng N., Culpepper J., Devos R., Richards G., Campfield L., Clark F. T. Cell. 83:1995;1263-1271.
17. Lee C. M., Campbell N. J., Williams B. J., Iversen L. L. Eur. J. Pharmacol. 130:1986;209-217.
18. Kemp B. E., Graves D. J., Benjamini E., Krebs E. G. J. Biol. Chem. 252:1977;4888-4894.
19. Blanar M. A., Rutter W. J. Science. 256:1992;1014-1018.
20. Kaelin W. G., Krek W., Sellers W. R., DeCaprio J. A., Ajchenbaum F. Cell. 70:1992;351-364.