Catalytic properties of an expressed and purified higher plant type ζ- carotene desaturase from Capsicum annuum

European Journal of Biochemistry

Volumn 265, Issue 1, 1999, Pages 376-383

  Breitenbach, Jürgen ^a   Kuntz, Marcel ^b   Takaichi, Shinichi ^c   Sandmann, Gerhard ^a  

^a GOETHE UNIVERSITY   (Germany)

^b UNIV GRENOBLE ALPES   (France)

^c NIPPON MEDICAL SCHOOL   (Japan)

Author keywords

carotene desaturase; Capsicum annuum; Isomeric substrate; Lycopene isomers; Quinone cofactor

Indexed keywords

CAROTENE; LYCOPENE; VEGETABLE PROTEIN;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; NONHUMAN; PEPPER; PHYTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

CAPSICUM; CAROTENOIDS; ESCHERICHIA COLI; ISOMERISM; KINETICS; OXIDOREDUCTASES; PLANTS, MEDICINAL; PLASTOQUINONE; RECOMBINANT PROTEINS; UBIQUINONE;

CAPSICUM ANNUUM; EMBRYOPHYTA; ESCHERICHIA COLI; TETRACIS;

EID: 0033214214     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00746.x     Document Type: Article

References (32)

1. 1. Sandmann, G. (1994) Carotenoid biosynthesis in microorganisms and plants. Eur. J. Biochem. 223, 7-24.
2. 2. Goodwin, T.W. (1980) The Biochemistry of the Carotenoids, Vol. 1, Plants, 2nd edn. Chapman & Hall, New York, USA.
3. 3. Woodall, A.A., Britton, G. & Jackson, M.J. (1997) Carotenoids and protection of phospholipids in solution or in liposomes against oxidation by peroxyl radicals: relationship between carotenoid structure and protective ability. Biochim. Biophys. Acta 1336, 575-586.
4. 4. Hirayama, O., Nakamura, K., Hamada, S. & Kobayashi, Y. (1994) Singlet oxygen quenching ability of naturally occurring carotenoids. Lipids 29, 149-150.
5. 5. Havaux, M. (1998) Carotenoids as membrane stabilizers in chloroplasts. Trends Plant Sci. 3, 147-151.
6. 6. Linden, H., Misawa, N., Chamovitz, D., Pecker, I., Hirschberg, J. & Sandmann, G. (1991) Functional complementation in Escherichia coli of different phytoene desarturase genes and analysis of accumulating carotenes. Z. Naturforsch. 46c, 1045-1051.
7. 7. Chamovitz, D., Pecker, I. & Hirschberg, J. (1991) The molecular basis of resistance to the herbicide norflurazon. Plant Mol. Biol. 16, 967-974.
8. 8. Linden, H., Misawa, N., Saito, T. & Sandmann, G. (1994) A novel carotenoid biosynthesis gene coding for ζ-carotene desaturase: functional expression, sequence and phylogenetic origin. Plant Mol. Biol. 24, 369-379.
9. 9. Breitenbach, J., Fernández-Gonzáles, B., Vioque, A. & Sandmann, G. (1998) A higher-plant type ζ-carotene desaturase in the cyanobacterium Synechocystis PCC6803. Plant Mol. Biol. 36, 725-732.
10. 10. Albrecht, M., Klein, A., Hugueney, H., Sandmann, G. & Kuntz, M. (1995) Molecular cloning and functional expression in E. coli of a novel plant enzyme mediating ζ-carotene desaturation. FEBS Lett. 372, 199-202.
11. 11. Scolnik, P.A. & Bartley, G.E. (1995) Nucleotide sequence of ζ-carotene desaturase (Accession no. U38550) from Arabidopsis. Plant Physiol. 109, 1499.
12. 12. Clough, J.M. & Pattenden, G. (1983) Stereochemical assignment of prolycopene and other poly-ζ-isomeric carotenoids in fruits of the tangerine tomato Lycopersicon esculentum var. 'Tangella'. J. Chem. Soc. Perkin Trans. I, 3011-3018.
13. 13. Bartley, G.E., Schmidhauser, T.J., Yanofsky, C. & Scolnik, P.A. (1990) Carotenoid desaturases from Rhodobacter capsulatus and Neurospora crassa are structurally and functionally conserved and contain domains homologous to flavoprotein disulfide oxido-reductases. J. Biol. Chem. 265, 16020-16024.
14. 14. Pecker, I., Chamovitz, D., Linden, H., Sandmann, G. & Hirschberg, J. (1992) A single polypeptide catalysing the conversion of phytoene to ζ-carotene is transcriptionally regulated during tomato fruit ripening. Proc. Natl Acad. Sci. USA 89, 4962-4966.
15. 15. Albrecht, M., Linden, H. & Sandmann, G. (1996) Biochemical characterization of purified ζ-carotene desaturase from Anabaena PCC7120 after expression in E. coli. Eur. J. Biochem. 236, 115-120.
16. 16. Maniatis, T., Fritsch, E.F. & Sambrook, J. (1982) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
17. 17. Bejarano, E.R., Govind, N.S. & Cerda-Olmedo, E. (1987) ζ-Carotene and other carotenes in a Phycomyces mutant. Photochemistry 26, 2251-2254.
18. 18. Blackwell, J.R. & Horgan, R. (1991) A novel strategy for production of a highly expressed recombinant protein in an active form. FEBS Lett. 295, 10-12.
19. 19. Lucht, J.M. & Bremer, E. (1994) Adaptation of Escherichia coli to high osmolarity environments: osmoregulation of the high-affinity glycine betaine transport sytem ProU. FEMS Microbiol. Rev. 14, 3-20.
20. 20. Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680-688.
21. 21. Petersen, G.L. (1977) Amplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346-356.
22. 22. Sander, L.C., Epler Sharpless, K., Craft, N.E. & Wise, S.A. (1994) Development of engineered stationary phases for the separation of carotenoid isomers. Anal. Chem. 66, 1667-1674.
23. 23. Takaichi, S. (1993) Usefulness of field desorption mass spectrometry in determining molecular masses of carotenoids, natural carotenoid derivatives and their chemical derivatives. Org. Mass Spectrom. 28, 785-788.
24. 24. Bartel, P.L., Chien, C.-T., Sternglanz, R. & Fields, S. (1993) Using the two-hybrid system to detect protein-protein interactions. In Cellular Interactions in Development: A Practical Approach (Hartley, D.A., ed.), pp. 153-179. Oxford University Press, Oxford, UK.
25. 25. Beyer, P., Mayer, M. & Kleinig, H. (1989) Molecular oxygen and the state of geometric isomerism of intermediates are essential in the carotene desaturation and cyclization reaction in daffodil chromoplasts. Eur. J. Biochem. 184, 141-150.
26. 26. Bartley, G.E., Scolnik, P.A. & Beyer, P. (1999) Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and ζ-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield pro-lycopene. Eur. J. Biochem. 259, 396-403.
27. 27. Sandmann, G. (1997) High level expression of carotenogenic genes for enzyme purification and biochemical characterization. Pure Appl. Chem. 66, 2163-2168.
28. 28. Goodwin, T.W. (1983) Development in carotenoid biochemistry over 40 years. Biochem. Soc. Trans. 11, 473-483.
29. 29. Cholett, R., Sandmann, G., Diethelm, R., Felix, H., Milzner, K. & Böger, P. (1990) ζ-Carotene accumulation and bleaching by new pyrimidine compounds. Pestic. Sci. 30, 326-329.
30. 30. Schneider, C., Böger, P. & Sandmann, G. (1997) Phytoene desaturase: heterologous expression in an active state, purification, and biochemical properties. Prot. Exp. Purif. 10, 175-179.
31. 31. Mayer, M.P., Beyer, P. & Kleinig, H. (1990) Quinone compounds are able to replace molecular oxygen as terminal electron acceptors in phytoene desaturation in chromoplasts of Narcissus pseudonarcissus L. Eur. J. Biochem. 191, 359-363.
32. 32. Carol, P., Stevenson, D., Bisanz, C., Breitenbach, J., Sandmann, G., Mache, R., Coupland, G. & Kuntz, M. (1999) Mutations in the Arabidopsis gene IMMUTANS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation. Plant Cell 11, 57-68.