Expression and partial purification of a recombinant secretory form of human liver carboxylesterase

Protein Expression and Purification

Volumn 17, Issue 1, 1999, Pages 16-25

  Miller, Amanda D ^a   Scott, David F ^c   Chacko, Terry L ^c   Maxwell, Donald M ^b   Schlager, John J ^b   Lanclos, Kenneth D ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

^b US ARMY MEDICAL RESEARCH INSTITUTE OF CHEMICAL DEFENSE   (United States)

^c NONE

Author keywords

Carboxylesterase; cDNA cloning; Cell culture; Protein purification; Site directed mutagenesis

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACID SUBSTITUTION; CARBOXY TERMINAL SEQUENCE; CARBOXYLESTERASE; COMPLEMENTARY DNA; DETOXIFICATION; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYMIC HYDROLYSIS; GENE OVEREXPRESSION; HUMAN CELL; HYDROPHOBICITY; ISOELECTRIC FOCUSING; LIVER; PLASMID; PROTEIN EXPRESSION; PROTEIN ISOLATION; RECOMBINANT PROTEIN; STEADY STATE; XENOBIOTIC METABOLISM;

EID: 0033212762     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1121     Document Type: Article

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