Quick bacterial microwave fixation technique for scanning electron microscopy

Microscopy Research and Technique

Volumn 46, Issue 4-5, 1999, Pages 338-339

  Fox, Norman E ^a   Demaree, Richard S ^a  

^a CALIFORNIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MICROWAVE FIXATION;

SCANNING ELECTRON MICROSCOPY;

CELL STRUCTURE; CONFERENCE PAPER; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; IMAGE QUALITY; MEMBRANE FILTER; MICROWAVE IRRADIATION; NONHUMAN; PRESERVATION; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TECHNIQUE; TISSUE FIXATION;

EID: 0033200364     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0029(19990815/01)46:4/5<338::AID-JEMT12>3.0.CO;2-L     Document Type: Conference Paper

References (11)

1. Amako K, Umeda A. 1977. Bacterial surfaces as revealed by the high resolution scanning electron microscope. J Gen Microbiol 98:297-299.
2. Galli D, Wirth R, Wanner G. 1989. Identification of aggregation substances of Enterococcus faecalis cells after induction by sex pheromones. An immunological and ultrastructural investigation. Arch Microbiol 151:486-490.
3. Giberson RT, Demaree RS Jr. 1995. Microwave fixation: understanding the variables to achieve rapid reproducible results. Microsc Res Tech 32: 246-254.
4. Giberson RT, Demaree RS. 1999. Electron microscopy methods and protocols. In: Methods in molecular biology, Vol. 117. Totowa, NJ: Humana Press Inc. p145-158.
5. Giberson RT, Smith RL, Demaree RS Jr. 1995. Three hour microwave tissue processing for transmission electron microscopy: from unfixed tissues to sections. Scanning 17(Suppl. 5):26-27.
6. Giberson RT, Demaree RS Jr, Nordhausen RW. 1997. Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diagn Invest 9:61-67.
7. Krogstad DJ, Smith RM, Moellering RC, Parquette AR. 1980. Visualization of cell-cell contact during conjugation in Streptococcus faecalis. J Bacteriol 141:963-967.
8. Login GR, Dvorak AM. 1994. Methods of microwave fixation for microscopy. A review of research and clinical applications: 1970-1992. Prog Histochem Cytochem 27:1-127.
9. Murray BE. 1998. Diversity among multidrug-resistant enterococci. Emerg Infect Dis 4:37-47.
10. Olmsted SB, Erlandsen SL, Dunny GM, Wells CL. 1993. High-resolution visualization by field emission scanning electron microscopy of Enterococcus faecalis surface proteins encoded by the pheromone-inducible conjugative plasmid pCFlO. J Bacteriol 175: 6229-6237.
11. Wanner G, Formanek H, Galli D, Wirth R. 1989. Localization of aggregation substances of Enterococcus faecalis after induction by sex pheromones. An ultrastructural comparison using immuno labelling, transmission and high resolution scanning electron microscopic techniques. Arch Microbiol 151:491-497.