The crystal structure of a wheat nonspecific lipid transfer protein (ns- LTP1) complexed with two molecules of phospholipid at 2.1 Å resolution

European Journal of Biochemistry

Volumn 264, Issue 2, 1999, Pages 562-568

  Charvolin, Delphine ^a   Douliez, Jean Paul ^b   Marion, Didier ^b   Cohen Addad, Claudine ^a   Pebay Peyroula, Eva ^a,c  

^a UNIV GRENOBLE ALPES   (France)

^b U Biochim Technol des Proteines   (France)

^c CEA GRENOBLE   (France)

Author keywords

Crystallography; Hydrophobic cavity; Lipid transfer protein; Phospholipid; Wheat

Indexed keywords

PHOSPHOLIPID; STEROL CARRIER PROTEIN 2;

ARTICLE; BINDING AFFINITY; BINDING SITE; COMPLEX FORMATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; CRYSTALLOGRAPHY; HYDROPHOBICITY; NONHUMAN; PLANT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; WHEAT;

TRITICUM AESTIVUM;

EID: 0033199578     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00667.x     Document Type: Article

References (35)

1. Kader, J.C. (1996) Lipid-transfer protein in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 627-654.
2. Dubreil, L., Gaborit, T., Bouchet, B., Gallant, D.J., Broekaert, W.F., Quillien, L. & Marion, D. (1998) Spatial and temporal distribution of the major isoforms of puroindolines (puroindoline-a and puroindoline-b) and non-specific lipid transfer protein (ns-LTP1) of Triticum aestivum seeds. Relationships with their in vitro antifungal properties. Plant Sci. 138, 121-135.
3. Sterk, P., Booij, H., Schellekens, G.A., Van Kammen, A. & De Vries, S.C. (1991) Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3, 907-921.
4. Garcia-Olmedo, F., Molina, F., Segura, A. & Moreno, M. (1995) The defensive role of non specific lipid transfer proteins in plants. Trends Microbiol. 3, 72-74.
5. 1H-NMR data. Eur. J. Biochem. 226, 413-422.
6. Shin, D.H., Lee, J.Y., Hwang, K.Y., Kim, K.K. & Suh, S.W. (1995) High resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings. Structure 3, 189-199.
7. Gomar, J., Petit, M.-C., Sodano, P., Sy, D., Marion, D., Kader, J.-C., Vovelle, F. & Ptak, M. (1996) Solution structure and lipid binding of a nonspecific lipid transfer protein extracted from maize seeds. Protein Sci. 5, 565-577.
8. Heinemann, B., Andersen, K.V., Nielsen, P.R., Bech, L.M. & Poulsen, F.M. (1996) Structure in solution of a four-helix lipid binding protein. Protein Sci. 5, 13-23.
9. Lee, J.Y., Min, K., Cha, H., Shin, D.H., Hwang, K.Y. & Suh, S.W. (1998) Rice non-specific lipid transfer protein: the 1.6 Å crystal structure in the unliganded state reveals a small hydrophobic cavity. J. Mol. Biol. 276, 437-448.
10. Rickers, I., Tober, I. & Spener, F. (1984) Purification and binding characteristics of a basic fatty acid binding protein from Avena sativa seedlings. Biochim. Biophys. Acta 794, 313-319.
11. Østergaard, J., Vergnolle, C., Schoentgen, F. & Kader, J.C. (1993) Acyl-binding/lipid-transfer proteins from rape seedlings, a novel category of proteins interacting with lipids. Biochim. Biophys. Acta 1170, 109-117.
12. Meijer, E.A., de Vries, S.C., Sterk, P., Gadella, D.W. Jr, Wirtz, K.W. & Hendriks, T. (1993) Characterization of the non-specific lipid transfer protein EP2 from carrot (Daucus carota L.). Mol. Cell. Biochem. 123, 159-166.
13. Lerche, M.H., Kragelund, B.B., Bech, L.M. & Poulsen, F.M. (1997) Barley lipid-transfer protein complexed with palmitoyl CoA: the structure reveals a hydrophobic binding site that can expand to fit both large and small lipid-like ligands. Structure 5, 291-306.
14. Désormeaux, A., Blochet, J.E., Pézolet, M. & Marion, D. (1992) Amino acid sequence of a non-specific wheat phospholipid transfer protein and its conformation as revealed by infrared and Raman spectroscopy. Role of disulfide bonds and phospholipids in the stabilization of α-helix structure. Biochim. Biophys. Acta 1121, 137-152.
15. 1H NMR and fluorescence studies of the complexation of DMPG by wheat non-specific lipid transfer protein. Global fold of the complex. FEBS Lett. 416, 130-134.
16. Tassin, S., Broeckaert, W.F., Marion, D., Acland, D.P., Ptak, M., Vovelle, F. & Sodano, P. (1998) Solution structure of Ace-AMPI, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins. Biochemistry 37, 3623-3637.
17. Baud, F., Pebay-Peyroula, E., Cohen-Addad, C., Odani, S. & Lehmann, M.S. (1992) Crystal structure of hydrophobic protein from soybean; a member of a new cysteine-rich family. J. Mol. Biol. 213, 877-887.
18. Blochet, J.E., Chevalier, C., Forest, E., Pebay-Peyroula, E., Gautier, M.F., Joudrier, P., Pézolet, M. & Marion, D. (1993) Complete amino acid sequence of puroindoline, a new basic and cystine-rich protein with a unique tryptophan-rich domain, isolated from wheat endosperm by Triton XI14 phase partitioning. FEBS Lett. 3, 336-340.
19. Pebay-Peyroula, E., Cohen-Addad, C., Lehmann, M.S. & Marion, D. (1992) Crystallographic data for the 9000 dalton wheat non-specific phospholipid transfer protein. J. Mol. Biol. 226, 563-564.
20. Messerschmidt, A. & Pflugrath, J.W. (1987) Crystal orientation and X-ray pattern prediction routines for area-detector diffractometer systems in macromolecular crystallography. J. Appl. Crystallogr. 20, 306-315.
21. Kabsch, W. (1988) Evaluation of single crystal X-ray diffraction data from a position-sensitive detector. J. Appl. Crystallogr. 21, 2577-2637.
22. Collaborative Computational Project, no. 4 (1994) The CCP4 suite programs for protein cristallography. Acta Crystallogr., Sect. D 50, 760-763.
23. Navaza, J. (1994) AMoRe: an Automated Package for Molecular replacement. Acta Crystallogr., Sect. A 50, 157-163.
24. Brunger, AT. (1992) X-PLOR, Version 3.1: a System for X-Ray Crystallography and NMR. Yale University Press, New Haven, CT, USA.
25. Jones, TA., Zou, J.Y., Cowan, S.W. & Kjeldgaard, M. (1991) Improved methods for the building of protein models in electron density maps and the location of errors in these models. Acta Crystallogr., Sect. A 47, 110-119.
26. Engh, R.A. & Huber, R. (1991) Accurate bond and angle parameters for X-ray protein structure refinement. Acta Crystallogr., Sect. A 47, 392-400.
27. Lamzin, V.S. & Wilson, K.S. (1993) Automated refinement of protein models. Acta Crystallogr., Sect. D 49, 129-147.
28. Pascher, I., Sundell, S. & Hauser, H. (1981) Polar group interaction and molecular packing of membrane lipids. The crystal structure of lysophosphatidylethanolamine. J. Mol. Biol. 153, 807-824.
29. Laskowski, R.A., MacArthur, M.W., Moss, D.S. & Thornton, J.M. (1993) PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallogr. 26, 283-291.
30. Kraulis, P.J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr. 42, 946-950.
31. Nicholls, A., Bharadwaj, R. & Honig. B. (1993) GRASP: graphical representation and analysis of surface properties. Biophys. J. 64, 166-170.
32. Kabsch, W. & Sander, C. (1983) Dictionnary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers 22, 2577-2637.
33. Michon, T., Compoint, G., Douliez, J.P., Sodano, P., Ptak, M. & Marion, D. (1998) Lipid transfer protein (LTP) from wheat kernel possesses a weak, esterase like activity towards short chain fatty acid esters. Plant Proteins from European Crops (Gueguen, J. & Popineau, Y., eds), pp. 36-40. Springer, Berlin.
34. Zachowski, A., Guerbette, F., Grosbois, M., Jolliot-Croquin, A. & Kader, J.C. (1998) Characterisation of acyl binding by a plant lipid- transfer protein. Eur. J. Biochem. 257, 443-448.
35. Curry, S., Mandelkow, H., Brick, P. & Franks, N. (1998) Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites. Nat. Struct. Biol. 9, 827-835.