Evaluation of a nested PCR for detection of Pneumocystis carinii in serum from immunocompromised patients

Journal of Eukaryotic Microbiology

Volumn 46, Issue 5, 1999, Pages

  Matos, Olga ^a   Lundgren, Bettina ^b   Caldeira, Luis ^c   Mansinho, Kamal ^d   Aguiar, Pedro ^a   Forte, Miguel ^c   Antunes, Francisco ^a,c  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

^b GENTOFTE UNIVERSITY HOSPITAL   (Denmark)

^c HOSPITAL DE SANTA MARIA   (Portugal)

^d HOSPITAL EGAS MONIZ   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

DISEASE DETECTION; DISEASE DIAGNOSIS; PNEUMOCYSTIS CARINII; POLYMERASE CHAIN REACTION;

AIDS-RELATED OPPORTUNISTIC INFECTIONS; DNA, FUNGAL; EVALUATION STUDIES; FUNGEMIA; HIV SERONEGATIVITY; HUMANS; IMMUNOCOMPROMISED HOST; LUNG; PNEUMOCYSTIS; PNEUMONIA, PNEUMOCYSTIS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY;

ANIMALIA; PNEUMOCYSTIS CARINII;

EID: 0033198394     PISSN: 10665234     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (7)

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2. Dohn, M. N. & Frame, P. T. 1994. Clinical manifestations in adults. In Pneumocystis carinii Pneumonia, 2nd edn. Edited by Walzer P.D. New York: Marcel Dekker, pp 331-359.
3. Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B. & Erlich, H. A. 1986. Analysis of Enzymatically Amplified -globin and HLA-DQ DNA with Allele-Specific Oligonucleotide Probes. Nature, 324: 163-166.
4. Schluger, N., Godwin, T., Sepkowitz, K., Armstrong, D., Bernard, E., Rifkin, M., Cerami, A. & Bucala, R. 1992. Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia. J. Exp. Med., 176:1327-1333.
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6. Tamburrini, E., Mencarini, P., Visconti, E., Zolfo, M., De Luca, A., Siracusano, A., Ortona, L., Margutti, P. & Wakefield, A. E. 1996. Comparison of Two PCR Methods for Detection of Pneumocystis carinii in Bronchoalveolar Lavage Fluid. J. Euk. Microbiol., 43: 20S.
7. Wakefield, A. E., Pixley, F. J., Banerji, S., Sinclair, K., Miller, R. F., Moxon, E. R. & Hopkin, J. M. 1990. Amplification of Mitochondrial Ribosomal RNA Sequences from Pneumocystis carinii DNA of Rat and Human Origin. Mol. Biochem. Parasitol., 43: 69-76.