Purification of α-N-acetyl-galactosaminidase from Aspergillus niger and its use in the synthesis of GalNAc-α-(1→O)-serine

Enzyme and Microbial Technology

Volumn 25, Issue 6, 1999, Pages 483-488

  Naundorf, Andreas ^a   Ajisaka, Katsumi ^a  

^a MEIJI DAIRIES CORPORATION   (Japan)

Author keywords

N Acetyl galactosaminidase; Aspergillus niger; Enzymatic synthesis; Gram scale; O Glycopeptide conjugate; Purification

Indexed keywords

ACTIVATED CARBON; BIOSYNTHESIS; COLUMN CHROMATOGRAPHY; FUNGI; HYDROLYSIS; NITROGEN COMPOUNDS; PH EFFECTS; PURIFICATION; THERMODYNAMIC STABILITY;

ACETYLGALACTOSAMINIDASE; REVERSE HYDROLYSIS;

ENZYMES;

ALPHA N ACETYLGALACTOSAMINIDASE; GLYCOPEPTIDE;

ARTICLE; ASPERGILLUS NIGER; CARBOHYDRATE SYNTHESIS; COLUMN CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; REACTION ANALYSIS;

ASPERGILLUS NIGER;

EID: 0033198373     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(99)00073-3     Document Type: Article

References (10)

1. Bahl O.P., Agrawal K.M.L. α-galactosidase, β-galactosidase, and β-N-acetylglucosaminidase from Aspergillus Niger. Methods Enzymol. 28:1972;728-734.
2. Bahl O.P. 1,2-α-L-fucosidase from Aspergillus Niger. Methods Enzymol. 28:1972;738-743.
3. Matta K.L., Bahl O.P. α-Mannosidase from Aspergillus Niger. Methods Enzymol. 28:1972;749-755.
4. Swaminathan N., Matta K.L., Bahl O.P. 1,2-α-D-Mannosidase from Aspergillus Niger. Methods Enzymol. 28:1972;744-749.
5. McDonald M.J., Bahl O.P. α-N-acetylglucosaminidase from Aspergillus Niger. Methods Enzymol. 28:1972;734-738.
6. Kubo S. Changes in the specificity of blood groups induced by enzymes from soil fungi. J Forensic Sci. 34:1989;96-104.
7. Weissmann B., Hinrichsen D.F. Mammalian α-acetylgalactosaminidases. occurrence, partial purification, and action on linkages in submaxillary mucins. Biochemistry. 8:1969;2034-2043.
8. Johansson E., Hedbys L., Larsson P-O. Enzymatic synthesis of monosaccharide-amino acid conjugates. Enzyme Microb Technol. 13:1991;781-787.
9. Ajisaka K., Shirakabe M. Regioselective synthesis of α-L-fucosyl-containing disaccharides by use of α-L-fucosidases of various origin. Carbohydrate Res. 224:1992;291-299.
10. Bradford M.M. A rapid, and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.