Assay of dihydrofolate reductase activity by monitoring tetrahydrofolate using high-performance liquid chromatography with electrochemical detection

Analytical Biochemistry

Volumn 272, Issue 2, 1999, Pages 143-148

  Aiso, Kenji ^a   Nozaki, Tomoko ^a   Shimoda, Minoru ^a   Kokue, Eiichi ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REDUCTION;

DIHYDROFOLATE REDUCTASE; ELECTROCHEMICAL DETECTION; MICHAELIS-MENTEN KINETIC; RATE OF REDUCTION; SODIUM ASCORBATE; SPECTROPHOTOMETRIC ASSAYS; SUBSTRATE CONCENTRATIONS; TETRAHYDROFOLATES;

CHEMICAL DETECTION;

DIHYDROFOLATE REDUCTASE; TETRAHYDROFOLIC ACID;

ANIMAL TISSUE; ARTICLE; ELECTROCHEMICAL ANALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIVER HOMOGENATE; MICHAELIS MENTEN KINETICS; NONHUMAN; PRIORITY JOURNAL; RAT; REDUCTION; SPECTROPHOTOMETRY;

ANIMALIA;

EID: 0033180313     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4174     Document Type: Article

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