Kinetically controlled syntheses catalyzed by proteases in reverse micelles and separation of precursor dipeptides of RGD

Enzyme and Microbial Technology

Volumn 25, Issue 3-5, 1999, Pages 310-315

  Chen, Yu Xin ^a   Zhanga, Xue Zhong ^a   Chen, Song Ming ^a   You, De Lin ^a   Wu, Xiao Xia ^a   Yang, Xue Cheng ^a   Guan, Wen Zeng ^a  

^a JILIN UNIVERSITY   (China)

Author keywords

Dipeptide syntheses; Organic solvent; Proteases; Reverse micelles

Indexed keywords

AROMATIC HYDROCARBONS; BIOSYNTHESIS; ENZYME IMMOBILIZATION; MICELLES; MOISTURE; ORGANIC SOLVENTS; PH EFFECTS; PROTEINS; SEPARATION; SODIUM COMPOUNDS; THERMAL EFFECTS;

CHYMOTRYPSIN; DIPEPTIDE SYNTHESIS; PRECURSOR DIPEPTIDE; PROTEASES; REVERSE MICELLES; SILICA GEL COLUMN;

ENZYME KINETICS;

ARGINYLGLYCYLASPARTIC ACID; DIPEPTIDE; PROTEINASE;

ARTICLE; CHEMICAL REACTION KINETICS; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; MICELLE; PEPTIDE SYNTHESIS; REACTION ANALYSIS; TECHNIQUE;

EID: 0033179764     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(99)00045-9     Document Type: Article

References (18)

1. Saiki I., Yoneda J., Igarashi Y., Aoki M., Kusunose N., Ono K., Azuma I. Antimentastatic activity of polymeric RGDT peptides conjugated with poly (ethylene glycol). Jpn J Cancer Res. 84:1993;558-565.
2. Kumagai H., Tajima M., Ueno Y., Giga-Hama Y., Ohba M. Effect of cyclic RGD peptide on cell adhesion and tumor metastasis. Biochem Biophys Res Commun. 177:1991;74-82.
3. Humphries M.J., Olden K., Yamada K.M. A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science. 233:1986;467-470.
4. Saiki I., Matsumoto Y., Murata J., Makabe T., Yoneda J., Okuyama H., Kimizuka F., Ishizaki Y., Kato I., Azuma I. Recombinant fusion polypeptide with cell- and heparin-binding domains of fibronectin inhibits liver metastasis of L5178Y-ML25 lymphoma cells. Jpn J Cancer Res. 82:1991;1120-1129.
5. Gill I., López-Fandiño R., Jorb X., Vulfson E.N. Biologically active peptides and enzymatic approaches to their production. Enzyme Microb Technol. 18:1996;162-183.
6. Monter B., Herzog B., Stehle P., Fürst P. Kinetically controlled synthesis of dipeptides using ficin as biocatalyst. Biotechnol Appl Biochem. 14:1991;183-191.
7. Zhang X.-Z., Wang X., Chen S., Fu X., Wu X., Li C. Protease-catalyzed small peptide synthesis in organic media. Enzyme Microb Technol. 19:1996;538-544.
8. Chen S.-T., Chen S.-Y., Wang K.-T. Kinetically controlled peptide bond formation in anhydrous alcohol catalyzed by the industrial protease alcalase. J Org Chem. 57:1992;6960-6965.
9. Lüthi P., Luisi P.L. Enzymatic synthesis of hydrocarbon-soluble peptides with reverse micelles. J Am Chem Soc. 106:1984;7285-7286.
10. 2 in reversed micelles. Enzyme Microb Technol. 14:1992;117-124.
11. Serralheiro M.L., Prazeres D.M.F., Cabral J.M.S. Dipeptide synthesis and separation in a reversed micellar membrane reactor. Enzyme Microb Technol. 16:1994;1064-1073.
12. Chen YX, Zhang XZ, Zheng K, Chen S-M, Wang Q-C Wu X-X. Protease-catalyzed synthesis of precursor dipeptides of RGD with reverse micelles. Enzyme Microb Technol In press.
13. Arnon B. In: (Perlmann GE, Lorand L, editors) Methods in enzymology XIX, New York: Academic Press, 1970, p. 226-44.
14. Kullmann W. Enzymatic synthesis of Leu- and Met-enkephalin. Biochem Biophys Res Commun. 91:1979;693-698.
15. Schellenberger V., Jakubke H.-D. Protease-catalyzed kinetically controlled peptide synthesis. Angew Chem Int Ed Engl. 30:1991;1437-1449.
16. Gololobov M.Y., Kozlova E.V., Borisov I.L., Schellenberger U., Schellenberger V., Jakubke H.-D. How to predict product yield in kinetically controlled enzymatic peptide synthesis. Biotechnol Bioeng. 40:1992;432-436.
17. Schwarz A., Steinke D., Kula M.-R., Wandrey C. Optimization of enzyme-mediated peptide bond formation. Biotechnol Appl Biochem. 12:1990;188-195.
18. Chen S.-T., Chen S.-Y., Chen H.J., Huang H.-C., Wang K.-T. Probing the s-1′ subsite selectivity of an industrial alkalin protease in anhydrous t-butanol. Biolog Med Chem Lett. 3:1993;727-733.