Nicotinic receptor assembly requires multiple regions throughout the γ subunit

Journal of Neuroscience

Volumn 19, Issue 15, 1999, Pages 6298-6308

  Eertmoed, Alison L ^a   Green, William N ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

Acetylcholine; Assembly; Conformational changes; Nicotinic receptors; Protein folding; bungarotoxin

Indexed keywords

ALPHA BUNGAROTOXIN; BUNGAROTOXIN RECEPTOR; NICOTINIC RECEPTOR; RECEPTOR SUBUNIT;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; TORPEDO;

EID: 0033179069     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.19-15-06298.1999     Document Type: Article

References (42)

1. Blount P, Merlie JP (1991) BiP associates with newly synthesized subunits of the mouse muscle nicotinic receptor. J Cell Biol 113:1125-1132.
2. Blount P, Smith MM, Merlie JP (1990) Assembly intermediates of the mouse muscle nicotinic acetylcholine receptor in stably transfected fibroblasts. J Cell Biol 111:2601-2611.
3. Changeux JP, Edelstein SJ (1998) Allosteric receptors after 30 years. Neuron 21:959-980.
4. Claudio T, Raftery M (1977) Immunological comparison of acetylcholine receptors and their subunits from species of electric ray. Arch Biochem Biophys 181:484-489.
5. Claudio T, Green WN, Hartman DS, Hayden D, Paulson HL, Sigworth FJ, Sine SM, Swedlund A (1987) Genetic reconstitution of functional acetylcholine receptor channels in mouse fibroblasts. Science 238:1688-1694.
6. Deal KK, Lovinger DM, Tamkun MM (1994) The brain Kv1.1 potassium channel: in vitro and in vivo studies on subunit assembly and post-translational processing. J Neurosci 14:1666-1676.
7. Eertmoed AL, Vallejo YF, Green WN (1998) Transient expression of heteromeric ion channels. Methods Enzymol 293:564-585.
8. Forsayeth JR, Gu Y, Hall ZW (1992) BiP forms stable complexes with unassembled subunits of the acetylcholine receptor in transfected COS cells and in C2 muscle cells. J Cell Biol 117:841-847.
9. Gelman MS, Chang W, Thomas DY, Bergeron JJ, Prives JM (1995) Role of the endoplasmic reticulum chaperone calnexin in subunit folding and assembly of nicotinic acetylcholine receptors. J Biol Chem 270:15085-15092.
10. Green WN, Claudio T (1993) Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially. Cell 74:57-69.
11. Green WN, Millar NS (1995) Ion-channel assembly. Trends Neurosci 18:280-287.
12. Green WN, Wanamaker CP (1997) The role of the cysteine loop in acetylcholine receptor assembly. J Biol Chem 272:20945-20953.
13. Green WN, Wanamaker CP (1998) Formation of the nicotinic acetylcholine receptor binding sites. J Neurosci 18:5555-5564.
14. Gu Y, Forsayeth JR, Verrall S, Yu XM, Hall ZW (1991) Assembly of mammalian muscle acetylcholine receptors in transfected COS cells. J Cell Biol 114:799-807.
15. Gullick WJ, Lindstrom JM (1983) Mapping the binding of monoclonal antibodies to the acetylcholine receptor from Torpedo californica. Biochemistry 22:3312-3320.
16. A subunits specify their selective interaction in vitro. J Neurochem 70:40-46.
17. Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
18. Hurtley SM, Helenius A (1989) Protein oligomerization in the endoplasmic reticulum. Annu Rev Cell Biol 5:277-307.
19. Karlin A, Akabas MH (1995) Towards a structural basis for the function of nicotinic acetylcholine receptors and their cousins. Neuron 15:1231-1244.
20. Keller SH, Lindstrom J, Taylor P (1996) Involvement of the chaperone protein calnexin and the acetylcholine receptor β-subunit in the assembly and cell surface expression of the receptor. J Biol Chem 271:22871-22877.
21. Kreienkamp HJ, Maeda RK, Sine SM, Taylor P (1995) Intersubunit contacts governing assembly of the mammalian nicotinic acetylcholine receptor. Neuron 14:635-644.
22. Kuhse J, Laube B, Magalei D, Betz H (1993) Assembly of the inhibitory glycine receptor: identification of amino acid sequence motifs governing subunit stoichiometry. Neuron 11:1049-1056.
23. Kurosaki T, Fukuda K, Konno T, Mori Y, Tanaka K, Mishina M, Numa S (1987) Functional properties of nicotinic acetylcholine receptor subunits expressed in various combinations. FEBS Lett 214:253-258.
24. Lee BS, Gunn RB, Kopito RR (1991) Functional differences among nonerythroid anion exchangers expressed in a transfected human cell line. J Biol Chem 266:11448-11454.
25. Lindstrom JM (1995) Nicotinic acetylcholine receptors. In: Handbook of receptors and channels. Ligand- and voltage-gated ion channels (North RA, ed), pp 153-175. Boca Raton, FL: CRC.
26. Margolskee RF, McHendry-Rinde B, Horn R (1993) Panning transfected cells for electrophysiological studies. Biotechniques 15:906-911.
27. Merlie JP, Lindstrom J (1983) Assembly in vivo of mouse muscle acetylcholine receptor: identification of an α-subunit species that may be an assembly intermediate. Cell 34:747-757.
28. Mihovilovic M, Richman DP (1987) Monoclonal antibodies as probes of the α-bungarotoxin and cholinergic binding regions of the acetylcholine receptor. J Biol Chem 262:4978-4986.
29. Paulson HL, Ross AF, Green WN, Claudio T (1991) Analysis of early events in acetylcholine receptor assembly. J Cell Biol 113:1371-1384.
30. Ross AF, Green WN, Hartman DS, Claudio T (1991) Efficiency of acetylcholine receptor subunit assembly and its regulation by cAMP. J Cell Biol 113:623-636.
31. Saedi MS, Conroy WG, Lindstrom J (1991) Assembly of Torpedo acetylcholine receptors in Xenopus oocytes. J Cell Biol 112:1007-1015.
32. Sine SM (1993) Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of residues that determine curare selectivity. Proc Natl Acad Sci USA 90:9436-9440.
33. Sine SM, Claudio T (1991) Gamma- and delta-subunits regulate the affinity and the cooperativity of ligand binding to the acetylcholine receptor. J Biol Chem 266:19369-19377.
34. Smith MM, Lindstrom J, Merlie JP (1987) Formation of the α-bungarotoxin binding site and assembly of the acetylcholine receptor subunits occur in the endoplasmic reticulum. J Biol Chem 262:4367-4376.
35. Sumikawa K, Gehle VM (1992) Assembly of mutant subunits of the nicotinic acetylcholine receptor lacking the conserved disulfide loop structure. J Biol Chem 267:6286-6290.
36. Sumikawa K, Nishizaki T (1994) The amino acid residues 1-128 in the alpha subunit of the nicotinic acetylcholine receptor contain assembly signals. Brain Res Mol Brain Res 25:257-264.
37. Tzartos SJ, Lindstrom JM (1980) Monoclonal antibodies used to probe acetylcholine receptor structure: localization of the main immunogenic region and detection of similarities between subunits. Proc Natl Acad Sei USA 77:755-759.
38. Verrall S, Hall ZW (1992) The N-terminal domains of acetylcholine receptor subunits contain recognition signals for the initial steps of receptor assembly. Cell 68:23-31.
39. Yon J, Fried M (1989) Precise gene fusion by PCR. Nucleic Acids Res 17:4895.
40. Yu X-M, Hall ZW (1991) Extracellular domains mediating ∈ subunit interactions of muscle acetylcholine receptor. Nature 352:64-67.
41. Yu XM, Hall ZW (1994a) Amino- and carboxyl-terminal domains specify the identity of the delta subunit in assembly of the mouse muscle nicotinic acetylcholine receptor. Mol Pharmacol 46:964-969.
42. Yu XM, Hall ZW (1994b) A sequence in the main cytoplasmic loop of the alpha subunit is required for assembly of mouse muscle nicotinic acetylcholine receptor. Neuron 13:247-255.