Expression and activity of cyclic and linear analogues of esculentin-1, an anti-microbial peptide from amphibian skin

European Journal of Biochemistry

Volumn 263, Issue 3, 1999, Pages 921-927

  Ponti, Donatella ^a   Mignogna, Giuseppina ^a   Mangoni, Maria L ^b   De Biase, Daniela ^a   Simmaco, Maurizio ^b   Barra, Donatella ^a,c  

^a CNR   (Italy)

^b UNIVERSITY OF CHIETI   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Anti microbial peptide; Inclusion bodies; Intramolecular disulfide; Peptide expression; Salt inactivation

Indexed keywords

ANTIINFECTIVE AGENT; ESCULENTIN 1; ESCULENTIN 1 DERIVATIVE; INORGANIC SALT; MUTANT PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERICIDAL ACTIVITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISULFIDE BOND; DRUG POTENCY; DRUG SENSITIVITY; DRUG STRUCTURE; FROG; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAPHYLOCOCCUS AUREUS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMPHIBIAN PROTEINS; ANIMALS; ANTI-BACTERIAL AGENTS; ANTI-INFECTIVE AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BACTERIA; CANDIDA ALBICANS; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA PRIMERS; ERYTHROCYTES; HUMANS; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; POLYMERASE CHAIN REACTION; RANA ESCULENTA; RECOMBINANT PROTEINS; SKIN; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0033178536     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00597.x     Document Type: Article

References (26)

1. 1. Boman, H.G. (1995) Peptide antibiotics and their role in innate immunity. Annu. Rev. Immun. 13, 61-92.
2. 2. Lehrer, R.I. & Ganz, T. (1999) Antimicrobial peptides in mammalian and insect host defence. Curr. Opin. Immunol. 11, 23-27.
3. 3. Ojcius, D.M. & Young, J.D. (1991) Cytolytic pore-forming proteins and peptides: is there a common structural motifs? Trends Biochem. Sci. 16, 225-229.
4. 4. Shai, Y. (1995) Molecular recognition between membrane-spanning polypeptides. Trends Biochem. Sci. 20, 460-464.
5. 5. Levy, S.B. (1998) The challenge of antibiotic resistance. Scientific American, March issue, pp. 32-39.
6. 6. Piers, K.L., Brown, M.H. & Hancock, R.E. (1993) Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria. Gene 134, 7-13.
7. 7. Lee, J.H., Minn, I., Park, C.B. & Kim, S.C. (1998) Acidic peptide-mediated expression of the antimicrobial peptide buforin II as tandem repeats in Escherichia coli. Protein Expr. Purif. 12, 53-60.
8. 8. Zhang, L., Falla, T., Wu, M., Fidai, S., Burian, J., Kay, W. & Hancock, R.E. (1998) Determinants of recombinant production of antimicrobial cationic peptides and creation of peptide variants in bacteria. Biochem. Biophys. Res. Commun. 247, 674-680.
9. 9. Simmaco, M., Mignogna, G., Barra, D. & Bossa, F. (1994) Antimicrobial peptides from skin secretion of Rana esculenta. Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides. J. Biol. Chem. 269, 11956-11961.
10. 10. De Biase, D., Barra, D., Simmaco, M., John, R.A. & Bossa, F. (1995) Primary structure and tissue distribution of human 4-aminobutyrate aminotransferase. Eur. J. Biochem. 227, 476-480.
11. 11. Marston, F.A. (1986) The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 240, 1-12.
12. 12. Barra, D., Simmaco, M. & Boman, H.G. (1998) Gene-encoded peptide antibiotics and innate immunity. Do 'animalcules' have defence budgets? FEBS Lett. 430, 130-134.
13. 13. Hultmark, D., Engström, A., Andersson, K., Steiner, H., Bennich, H. & Boman, H.G. (1983) Insect immunity. Attacins, a family of antibacterial proteins from Hyalophora cecropia. EMBO J. 2, 571-576.
14. 14. Mignogna, G., Simmaco, M., Kreil, G. & Barra, D. (1993) Antibacterial and haemolytic peptides containing D-alloisoleucine from the skin of Bombina variegata. EMBO J. 12, 4829-4832.
15. 15. Lehrer, R.I., Barton, A. & Ganz, T. (1988) Concurrent assessment of inner and outer membrane permeabilization and bacteriolysis in E. coli by multiple-wavelength spectrophotometry. J. Immunol. Methods 108, 153-158.
16. 16. Shine, J. & Dalgarno, L. (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl Acad. Sci. USA 71, 1342-1346.
17. 17. Stormo, G.D., Schneider, T.D. & Gold, L.M. (1982) Characterization of translational initiation sites in E. coli. Nucleic Acids Res. 10, 2971-2996.
18. 18. Luo, P. & Baldwin, R.L. (1997) Mechanism of helix induction by trifluoroethanol: a framework for extrapolating the helix-forming properties of peptides from trifluoroethanol/water mixtures back to water. Biochemistry 36, 8413-8421.
19. 19. Goldschmidt, R. (1970) In vivo degradation of nonsense fragments in E. coli. Nature 228, 1151-1154.
20. 20. Lin, S. & Zabin, I. (1972) β-Galactosidase. Rates of synthesis and degradation of incomplete chains. J. Biol, Chem. 247, 2206-2211.
21. 21. Guan, C., Riggs, P.D. & Inouye, H. (1987) Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67, 21-30.
22. 22. Rang, C., Bes, M., Moar, W.J. & Frutos, R. (1997) Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies. FEBS Lett. 412, 587-591.
23. 23. Barra, D., Bossa, F., De Biase, D., Simmaco, M. & Ponti, D. (1998) Plasmid for the production of anti-microbial peptides in recombinant bacteria. Italian patent no. RM98A000393.
24. 24. Wu, M. & Hancock, R.E.W. (1999) Interaction of the cyclic antimicrobial cationic peptide bactenecin with the outer and cytoplasmic membrane. J. Biol. Chem. 274, 29-35.
25. 25. Harwig, S.L., Waring, A., Yang, H.J., Cho, Y., Tan, L. & Lehrer, R. (1996) Intramolecular disulfide bonds enhance the antimicrobial and lytic activities of protegrins at physiological sodium chloride concentrations. Eur. J. Biochem. 240, 352-357.
26. 26. Segura, A., Moreno, M., Molina, A. & Garcia-Olmedo, F. (1998) Novel defensin subfamily from spinach (Spinacia oleracea). FEBS Lett. 435, 159-162.