Astrocytes and neurons express the tight junction-specific protein occludin in vitro

Experimental Cell Research

Volumn 250, Issue 2, 1999, Pages 434-438

  Bauer, H ^a   Stelzhammer, W ^a   Fuchs, R ^a   Weiger, T M ^c   Danninger, C ^a   Probst, G ^a   Krizbai, I A ^a,b  

^a ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^b INSTITUTE OF BIOCHEMISTRY   (Hungary)

^c NONE

Author keywords

Astrocytes; Cell culture; Confocal laser microscopy; Neurons; Occludin; RT PCR; Tight junctions; Western blotting

Indexed keywords

OCCLUDIN;

ANIMAL CELL; ARTICLE; ASTROCYTE; EPITHELIUM CELL; MOUSE; NERVE CELL; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE CELL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TIGHT JUNCTION;

EID: 0033178485     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1999.4558     Document Type: Article

References (22)

1. Furuse M., Hirase T., Itoh M., Nagafuchi A., Yonemura S., Tsukita S., Tsukita S. Occludin: A novel integral membrane protein localizing at tight junctions. J. Cell Biol. 123:1993;1777-1788.
2. McCarthy K. M., Skare I. B., Stankewich M. C., Furuse M., Tsukita S., Rogers R. A., Lynch R. D., Schneeberger E. E. Occludin is a functional component of the tight junction. J. Cell Sci. 109:1996;2287-2298.
3. Wong V., Gumbiner B. M. A synthetic peptide corresponding to the extracellular domain of occludin perturbs the tight junction permeability barrier. J. Cell Biol. 136:1997;399-409.
4. Chen Y-h., Merzdorf C., Paul D. L., Goodenough D. A. COOH terminus of occludin is required for tight junction barrier function in early Xenopus embryos. J. Cell Biol. 138:1997;891-899.
5. Ando-Akatsuka Y., Saitou M., Hirase T., Kishi M., Sakakibara A., Itoh M., Yonemura S., Furuse M., Tsukita S. Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J. Cell Biol. 133:1996;43-47.
6. Furuse M., Itoh M., Hirase T., Nagafuchi A., Yonemura S., Tsukita S., Tsukita S. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin a tight junctions. J. Cell Biol. 127:1994;1617-1626.
7. Matter K., Balda M. Biogenesis of tight junctions: The C-terminal domain of occludin mediates basolateral targeting. J. Cell Sci. 111:1998;511-519.
8. Balda M. S., Whitney J. A., Flores C., Gonzalez S., Cereijido M., Matter K. Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein. J. Cell Biol. 134:1996;1031-1049.
9. Furuse M., Fujimoto K., Sato N., Hirase T., Tsukita S., Tsukita S. Overexpression of occludin, a tight junction-associated integral membrane protein, induces the formation of intracellular multilamellar bodies bearing tight junction-like structures. J. Cell Sci. 109:1996;429-435.
10. Hirase T., Staddon J. M., Saitou M., Ando-Akatsuka Y., Itoh M., Furuse M., Fujimoto K., Tsukita S., Rubin L. L. Occludin as a possible determinant of tight junction permeability in endothelial cells. J. Cell Sci. 110:1997;1603-1613.
11. Saitou M., Fujimoto K., Doi Y., Itoh M., Fujimoto T., Furuse M., Takano H., Noda T., Tsukita S. Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions. J. Cell Biol. 141:1998;397-408.
12. Tontsch U., Bauer H. C. Isolation, characterization, and long-term cultivation of porcine and murine cerebral capillary endothelial cells. Microvasc. Res. 37:1989;148-161.
13. Bauer H. C., Tontsch U. Glial conditioned medium and attachment to Con A are essential for long-term culture of cortical neurons. Int. J. Dev. Neurosci. 8:1990;151-158.
14. Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
15. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:1976;248-254.
16. Van Itallie C. M., Anderson J. M. Occludin confers adhesiveness when expressed in fibroblasts. J. Cell Sci. 110:1997;1113-1121.
17. Sakakibara A., Furuse M., Saitou M., Ando-Akatsuka Y., Tsukita Sh. Possible involvement of phosphorylation of occludin in tight junction formation. J. Cell Biol. 137:1997;1393-1401.
18. Saitou M., Ando-Akatsuka Y., Itoh M., Furuse M., Inazawa J., Fujimoto K., Tsukita S. Mammalian occludin in epithelial cells: Its expression and subcellular distribution. Eur. J. Cell Biol. 73:1997;222-231.
19. Howarth A. G., Hughes M. R., Stevenson B. R. Detection of the tight junction-associated protein ZO-1 in astrocytes and other nonepithelial cell types. Am. J. Physiol. 262:1992;C461-C469.
20. Howarth A. G., Singer K. L., Stevenson B. R. Analysis of the distribution and phosphorylation state of ZO-1 in MDCK and nonepithelial cells. J. Membr. Biol. 137:1994;261-270.
21. Howarth A. G., Stevenson B. R. Molecular environment of ZO-1 in epithelial and non-epithelial cells. Cell Motility Cytoskeleton. 31:1995;323-332.
22. Aaku-Saraste E., Hellwig A., Huttner W. B. Loss of occludin and functional tight junctions, but not ZO-1, during neural tube closure - Remodeling of the neuroepithelium prior to neurogenesis. Dev. Biol. 180:1996;664-679.