RecA interacts with Klenow and enhances fidelity of DNA synthesis in vitro

Journal of Molecular Microbiology and Biotechnology

Volumn 1, Issue 1, 1999, Pages 149-158

  Karthikeyan, G ^a   Lakshmikant, G S ^a   Wagle, Mahendra D ^a   Krishnamoorthy, G ^a   Rao, Basuthkar J ^a  

^a TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DNA; DNA DIRECTED DNA POLYMERASE BETA; EXONUCLEASE; RECA PROTEIN; SINGLE STRANDED DNA;

ARTICLE; BIOSYNTHESIS; DNA REPAIR; ESCHERICHIA COLI; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; DNA; DNA POLYMERASE I; DNA REPAIR; DNA, BACTERIAL; DNA, SINGLE-STRANDED; ESCHERICHIA COLI; EXONUCLEASES; MOLECULAR SEQUENCE DATA; REC A RECOMBINASES;

EID: 0033170905     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Asai, T., Bates, D.B., and Kogoma. T. 1994. DNA replication triggered by doublestranded breaks in E. coli: dependence on homologous recombination functions. Cell. 78: 1051-1061.
2. Brinkley, M. 1992. A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Bioconjugate Chem. 3: 2-13.
3. Cao, Y., and Kogoma, T. 1995. The mechanism of recA polA lethality: Suppression by RecA-independent recombination repair activated by the lexA (Def) mutation in Escherichia coli. Genetics. 139: 1483-1494.
4. Cox, M.M. 1993. Relating biochemistry to biology: How the recombinational repair function of RecA protein is manifested in its molecular properties. Bioessays. 15: 617-623.
5. Echols, H., and Goodman, M.F. 1991. Fidelity mechanisms in DNA replication. Annu. Rev. Biochem. 60: 477-511.
6. Egelmen, E.H. 1993. What do X-ray crystallographic and electroni microscopic structural studies of the RecA protein tell us about recombination. Curr. Opin. Struct Biol. 3: 189.
7. Gross, J.D., Grunstein J., and Witkin, E.M. 1971. Inviability of recA derivatives of the DNA polymerase mutant of De Lucia and Cairns. J. Mol. Biol. 58: 631634.
8. Harlow, E., and Lane, D. 1988. In: Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. p. 351.
9. Heyduk, T., and Lee, J.C. 1990. Application of fluorescence energy transfer and polarization to monitor Escherichia coli cAMP receptor protein and lac promoter interaction. Proc. Natl. Acad. Sci. USA. 87: 1744-1748.
10. Heyduk, T., Lee, J.C., Ebright, Y.W., Blatter, E.E., Zhou, Y., and Ebright, R.H. 1993. CAP interacts with RNA polymerase in solution in the absence of promoter DNA. Nature. 364: 548-549.
11. Heyduk, T., Ma, Y., Tang, H., and Ebright, R.H. 1997. Fluorescence anisotropy: Rapid, quantitative assay for protein-DNA and protein-protein interaction. Methods Enzymol. 274: 492-503.
12. Joyce, C.M., and Derbyshire, V. 1995. Purification of E. Coli DNA polymerase 1 and Klenow fragment. Methods in Enzymol. 262: 3-13.
13. Karthikeyan G, Wagle M.D., and Rao B.J. 1998. Non-Watson-Crick base pairs modulate homologous alignments in RecA pairing reactions. FEBS Lett. 425: 45-51
14. Kogoma, T. 1997. Stable DNA replication: Interplay between DNA replication, homologous recombination, and transcription. Microbiol. Mol. Biol. Rev. 61: 212-238.
15. Komberg, A., and Baker, T.A. 1992. In: DNA Replication 2nd Ed. W.H. Freeman and Company, New York.
16. Kowalczykowski, S.C., and Eggleston, A.K. 1994. Homologous pairing and DNA strandexchange proteins. Annu. Rev. Biochem. 63: 991-1043.
17. Kwok, R.P., Lundblad, J.R., Chrivia, J.C., Richards, J.P., Bachinger, H.P., Brennan, R.G., Roberts, S.G., Green, M.R., and Goodman, R.H. 1994. Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature. 370: 223-226.
18. Leahy, M.C., and Radding, C.M. 1986. Topography of the interaction of RecA protein with single stranded deoxyoligonucleotides. J. Biol. Chem. 261: 695-460.
19. L control. Biochem. 25: 21-25.
20. Lu, C., Scheuermann, R.H., and Echols, H. 1986. Capacity of RecA protein to bind preferentially to UV lesions and inhibit the editing subunit (epsilon) of DNA polymerase III: A possible mechanism for SOS-induced targeted mutagenesis. Proc Natl Acad. Sci. USA. 83: 619-623.
21. Lundblad, J.R., Kwok, R.P., Laurance, M.E., Harter, M.L., and Goodman, R.H. 1995. Adenoviral E1A-associated protein p300 as a functional homologue of the transcriptional coactivator CBP. Nature. 374: 8588.
22. Maxam, A.M., and Gilbert, W. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymol. 65: 499-560
23. Michaels, M.L., Lee, M.S., and Romano, L.J. 1986. Contrasting effects of Escherichia coli single-stranded DNA binding protein on synthesis by T7 DNA polymerase and Escherichia Coli DNA polymerase I (large fragment). Evidence that binding protein inhibits translesion synthesis by polymerase I. J. Biol. Chem. 261: 4847-4854.
24. Monk, M., and Kinross, J. 1972. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J. Bacteriol. 109: 971978.
25. Monk, M., Kinross, J., and Town, C. 1973. Deoxyribonucleic acid synthesis in recA and recB derivatives of an Escherichia coli K12 strain with a temperaturesensitive deoxyribonucleic acid polymerase I. J. Bacteriol. 114: 1014-1017.
26. Perez-Howard, G.M., Weil, P.A., and Beechem, J.M. 1995. Yeast TATA binding protein interaction with DNA: Fluorescence determination of oligomeric state, equilibrium binding, onrate, and dissociation kinetics. Biochem. 34: 8005-8017.
27. Periasamy, N., Doraiswamy, S., Maiya, G.B., and Venkataraman, D. 1988. Diffusion controlled reactions: Fluorescence quenching of cationic dyes by charged quenchers. J. Chem. Phys. 88: 1638-1651.
28. Pyles, E.A., and Lee J.C. 1996. Mode of selectivity in cyclic AMP receptor protein-dependent promoters in Escherichia coli. Biochem. 35: 11621172.
29. Radding, C.M. 1993. A universal recombination filament. Curr. Biol. 3: 358-360
30. Rao, B.J., Chiu, S.K., and Radding, C.M. 1993. Homologous recognition and triplex formation promoted by RecA protein between duplex oligonucleotides and singlestranded DNA. J. Mol. Biol. 229: 328-343.
31. Rupp, W.D., Wilde III, C.E., Reno, D.L., and Flanders, H.P. 1971. Exchanges between DNA strands in ultravioletirradiated Escherichia coli. J. Mol. Biol. 61: 25-44.
32. Shibata, T. Cunningham, R.P., and Radding, C.M. 1981. Homologous pairing in genetic recombination. Purification and characterization of Escherichia coli RecA protein. J. Biol. Chem. 256: 7557-7564.
33. Skarstad, K., and Boye, E. 1993. Degradation of individual chromosomes in recA mutants of Escherichia coli. J. Bacteriol. 175: 5505-5509.
34. Swaminathan, R., Nath, U., Udgaonkar, J.B., Periasamy, N., and Krishnamoorthy, G. 1996. Motional dynamics of a buried tryptophan reveals the presence of partially structured forms during denaturation of Barstar. Biochem. 35: 9150-9157.