Regulation of DNA replication by iterons: An interaction between the ori2 and incC regions mediated by RepE-bound iterons inhibits DNA replication of mini-F plasmid in Escherichia coli

EMBO Journal

Volumn 18, Issue 13, 1999, Pages 3856-3867

  Uga, Hitoshi ^a   Matsunaga, Fujihiko ^a   Wada, Chieko ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Copy number control; Cross link structure; Incompatibility; RepE protein; Replication control

Indexed keywords

BACTERIAL PROTEIN; PLASMID DNA;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DNA CROSS LINKING; DNA REPLICATION; ESCHERICHIA COLI; GENE MUTATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN;

DIMERIZATION; DNA REPLICATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; F FACTOR; GENE DOSAGE; GENE EXPRESSION; MODELS, GENETIC; MOLECULAR CONFORMATION; MUTATION; PHENOTYPE; PROTEIN BINDING; REGULATORY SEQUENCES, NUCLEIC ACID; REPLICATION ORIGIN; REPRESSOR PROTEINS; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); DNA VIRUSES; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033168690     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.13.3856     Document Type: Article

References (45)

1. Abeles, A.L. and Austin, S.J. (1991) Antiparallel plasmid-plasmid pairing may control P1 plasmid replication. Proc. Natl Acad. Sci. USA, 88, 9011-9015.
2. Abeles, A.L., Reaves, L.D., Youngren-Grimes, B. and Austin, S.J. (1995) Control of P1 plasmid replication by iterons. Mol. Microbiol., 18, 903-912.
3. Austin, S. and Nordström, K. (1990) Partition-mediated incompatibility of bacterial plasmids. Cell, 60, 351-354.
4. Bergquist, P.L., Downard, R.A., Caughey, P.A., Gardner, R.C. and Lane, H.E. (1981) Analysis of mini-F plasmid replication by transposition mutagenesis. J. Bacteriol., 147, 888-899.
5. Blasina, A., Kittell, B.L., Toukdarian, A.E. and Helinski, D.R. (1996) Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins. Proc. Natl Acad. Sci. USA, 93, 3559-3564.
6. Brendler, T.G., Abeles, A.L., Reaves, L.D. and Austin, S.J. (1997) The iteron bases and spacers of the P1 replication origin contain information that specifies the formation of a complex structure involved in initiation. Mol. Microbiol., 23, 559-567.
7. Chang, A.C. and Cohen, S.N. (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A miniplasmid. J. Bacteriol., 134, 1141-1156.
8. Chattoraj, D.K., Cordes, K. and Abeles, A.L. (1984) Plasmid P1 replication: negative control by repeated DNA sequences. Proc. Natl Acad. Sci. USA, 81, 6456-6460.
9. Chattoraj, D.K., Mason, R.J. and Wickner, S.H. (1988) Mini-Pi plasmid replication: the autoregulation-sequestration paradox. Cell, 52, 551-557.
10. Durland, R.H. and Helinski, D.R. (1990) Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number. J. Bacteriol., 172, 3849-3858.
11. Gordon, G.S., Sitnikov, D., Webb, C.D., Teleman, A., Straight, A., Losick, R., Murry, A.W. and Wright, A. (1997) Chromosome and low copy plasmid segregation in E.coli: visual evidence for distinct mechanisms. Cell, 90, 1113-1121.
12. BAD promoter. J. Bacteriol., 177, 4121-4130.
13. Inuzuka, M. and Wada, Y. (1985) A single amino acid alteration in the initiation protein is responsible for the DNA overproduction phenotype of copy number mutants of plasmid R6K. EMBO J., 4, 2301-2307.
14. Ishiai, M., Wada, C., Kawasaki, Y. and Yura, T. (1994) Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor). Proc. Natl Acad. Sci. USA, 91, 3839-3843.
15. Kawasaki, Y., Wada, C. and Yura, T. (1990) Roles of Escherichia coli heat shock proteins DnaK, DnaJ and GrpE in mini-F plasmid replication. Mol. Gen. Genet., 220, 277-282.
16. Kawasaki, Y., Wada, C. and Yura, T. (1991) Mini-F plasmid mutants able to replicate in the absence of sigma 32: mutations in the repE coding region producing hyperactive initiator protein. J. Bacteriol., 173, 1064-1072.
17. Kawasaki, Y., Wada, C. and Yura, T. (1992) Binding of RepE initiator protein to mini-F DNA origin (ori2). Enhancing effects of repE mutations and DnaJ heat shock protein. J. Biol. Chem., 267, 11520-11524.
18. Kawasaki, Y., Matsunaga, F., Kano, Y., Yura, T. and Wada, C. (1996) The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli. Mol. Gen. Genet., 253, 42-49.
19. Kittell, B.L. and Helinski, D.R. (1991) Iteron inhibition of plasmid RK2 replication in vitro: evidence for intermolecular coupling of replication origins as a mechanism for RK2 replication control. Proc. Natl Acad. Sci. USA, 88, 1389-1393.
20. Kline, B.C. (1979) Incompatibility between Flac, R386 and F:pSC101 recombinant plasmids: the specificity of F incompatibility genes. Plasmid, 2, 437-445.
21. Kline, B.C. (1985) A review of mini-F plasmid maintenance. Plasmid, 14, 1-16.
22. Kline, B.C. and Trawick, J. (1983) Identification and characterization of a second copy number control gene in mini-F plasmids. Mol. Gen. Genet., 192, 408-415.
23. Leung, D.W., Chen, E. and Goeddel, D.V. (1989) A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique, 1, 11-15.
24. Manis, J.J. and Kline, B.C. (1978) F plasmid incompatibility and copy number genes. Their map locations and interactions. Plasmid, 1, 492-502.
25. Masson, L. and Ray, D.S. (1986) Mechanism of autonomous control of the Escherichia coli F plasmid: different complexes of the initiator/ repressor protein are bound to its operator and to an F plasmid replication origin. Nucleic Acids Res., 14, 5693-5711.
26. Matsunaga, F., Kawasaki, Y., Ishiai, M., Nishikawa, K., Yura, T. and Wada, C. (1995) DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region. J. Bacteriol., 177, 1994-2001.
27. Matsunaga, F., Ishiai, M., Kobayashi, G., Uga, H., Yura, T. and Wada, C. (1997) The central region of RepE initiator protein of mini-F plasmid plays a crucial role in dimerization required for negative replication control. J. Mol. Biol., 274, 27-38.
28. McEachern, M.J., Bott, M.A., Tooker, P.A. and Helinski, D.R. (1989) Negative control of plasmid R6K replication: possible role of intermolecular coupling of replication origins. Proc. Natl Acad. Sci. USA, 86, 7942-7946.
29. Miron, A., Mukherjee, S. and Bastia, D. (1992) Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance. EMBO J., 11, 1205-1216.
30. Mukhopadhyay, G., Sozhamannan, S. and Chattoraj, D.K. (1994) Relaxation of replication control in chaperone-independent initiator mutants of plasmid P1. EMBO J., 13, 2089-2096.
31. Muraiso, K., Tokino, T., Murotsu, T. and Matsubara, K. (1987) Replication of mini-F plasmid in vitro promoted by purified E protein. Mol. Gen. Genet., 206, 519-521.
32. Niki, H. and Hiraga, S. (1997) Subcellular distribution of actively partitioning F plasmid during the cell division cycle in E.coli. Cell, 90, 951-957.
33. Nordström, K. (1990) Control of plasmid replication: how do DNA iterons set the replication frequency? Cell, 63, 1121-1124.
34. Pal, S.K. and Chattoraj, D.K. (1988) P1 plasmid replication: initiator sequestration is inadequate to explain control by initiator-binding sites. J. Bacteriol., 170, 3554-3560.
35. Papp, P.P., Mukhopadhyay, G. and Chattoraj, D.K. (1994) Negative control of plasmid DNA replication by iterons. Correlation with initiator binding affinity. J. Biol. Chem., 269, 23563-23568.
36. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
37. Seelke, R.W., Kline, B.C., Trawick, J.D. and Ritts, G.D. (1982) Genetic studies of F plasmid maintenance genes involved in copy number control, incompatibility, and partitioning. Plasmid, 7, 163-179.
38. Søgaard-Andersen, L., Rokeach, L.A. and Molin, S. (1984) Regulated expression of a gene important for replication of plasmid F in E.coli. EMBO J., 3, 257-262.
39. Swack, J.A., Pal, S.K., Mason, R.J., Abeles, A.L. and Chattoraj, D.K. (1987) P1 plasmid replication: measurement of initiator protein concentration in vivo. J. Bacteriol., 169, 3737-3742.
40. Tokino, T., Murotsu, T. and Matsubara, K. (1986) Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid. Proc. Natl Acad. Sci. USA, 83, 4109-4113.
41. Tolun, A. and Helinski, D.R. (1981) Direct repeats of the F plasmid incC region express F incompatibility. Cell, 24, 687-694.
42. Trawick, J.D. and Kline, B.C. (1985) A two-stage molecular model for control of mini-F replication. Plasmid, 13, 59-69.
43. Tsutsui, H., Fujiyama, A., Murotsu, T. and Matsubara, K. (1983) Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control. J. Bacteriol., 155, 337-344.
44. Wada, C. and Yura, T. (1984) Control of F plasmid replication by a host gene: evidence for interaction of the mafA gene product of Escherichia coli with the mini-F incC region. J. Bacteriol., 160, 1130-1136.
45. Wada, C., Imai, M. and Yura, T. (1987) Host control of plasmid replication: requirement for the sigma factor sigma 32 in transcription of mini-F replication initiator gene. Proc. Natl Acad. Sci. USA, 84, 8849-8853.