Directly observed membrane fusion between oppositely charged phospholipid bilayers

Journal of Membrane Biology

Volumn 170, Issue 1, 1999, Pages 27-38

  Pantazatos, D P ^a   MacDonald, R C ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Cationic lipid; EDOPC; Fluorescence microscopy; Hemifusion

Indexed keywords

ARTICLE; ELECTROPHORESIS; FLUORESCENCE MICROSCOPY; MEMBRANE BIOLOGY; MEMBRANE ELECTROPHYSIOLOGY; MEMBRANE FUSION;

FLUORESCEINS; FLUORESCENT DYES; LIPID BILAYERS; MEMBRANE FUSION; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLETHANOLAMINES; PHOSPHATIDYLGLYCEROLS; PHOSPHOLIPIDS; RHODAMINES;

EID: 0033166840     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002329900535     Document Type: Article

References (67)

1. 1. Allen, T.M., Cleland, L.G. 1980. Serum-induced leakage of liposome contents. Biochim. Biophys. Acta 597:418-426
2. 2. Anzai, K., Masumi, M., Kawasaki, K., Kirino, Y. 1993. Frequent fusion of liposomes to a positively charged planar bilayer without calcium ions. Journal of Biochemistry 114:487-491
3. 3. Ashley, G.W., Shida, M.M., Qiu, R., Lahiri, M.K., Levisay, P.C. Jones, R.D., Baker, K.A., MacDonald, R.C. 1996. Phosphatidylcholinium compounds: A new class of phospholipids with transfection activity and unusual physical properties. Biophys. J. 70:A88 (Abstr.)
4. 4. Bailey, A.L., Cullis, P.R. 1997. Membrane fusion with cationic liposomes: Effects of target membrane lipid composition. Biochemistry 36:1628-1634
5. 5. Barber, K., Mala, R.R. Lambert, M.P., Qiu, R., MacDonald, R.C. Klein, W.L. 1996. Delivery of membrane-impermeant fluorescent probes into living neural cell populations by lipotransfer. Neuroscience Letters 207;17-20
6. 6. Bearer, E.L., Düzgüneş, N., Friend, D.S., Papahadjopoulos, D. 1982. Fusion of phospholipid vesicles arrested by quick-freezing. The question of lipidic particles as intermediates in membrane fusion. Biochim. Biophys. Acta 693:93-98
7. 7. Bentz, J., Nir, S., Wilschut, J. 1983. Mass action kinetics of vesicle aggregation and fusion. Colloids and Surfaces 6:333-363
8. 8. Cevc, G. 1990. Membrane electrostatics. Biochim. Biophys. Acta 1031-3:311-382
9. 9. Chanturiya, A., Chernomordik, L.V., Zimmerberg, J. 1997. Flickering fusion pores comparable with initial exocytotic pores occur in protein-free phospholipid bilayers. Proc. Natl. Acad. Sci. USA 94:14423-14428
10. 10. Chernomordik, L. 1996. Nonbilayer lipids and biological fusion intermediates. Chem. Phys. Lipids 81:203-13
11. 11. Chernomordik, L., Chanturiya, A., Green, J., Zimmerberg, J. 1995. The hemifusion intermediate and its conversion to complete fusion: regulation by membrane composition. Biophys. J. 69:922-929
12. 12. Chernomordik, L., Kozlov, M.M., Melikyan, G.B., Abidor, I.G., Markin, V.S., Chizmadzhev, Y.A. 1985. The shape of lipid molecules and monolayer membrane fusion. Biochim. Biophys. Acta 812:643-655
13. 13. Chernomordik, L., Kozlov, M.M., Zimmerberg, J. 1995. Lipids in biological membrane fusion. J Membrane Biol. 146:1-14.
14. 14. Cohen, F.S., Akabas, M.H., Zimmerberg, J. Finkelstein, A. 1984. Parameters affecting the fusion of unilamellar phospholipid vesicles with planar bilayer membranes. J. Cell. Biol. 98:1054-1062
15. 15. Cohen, F.S., Niles, W.D. 1993. Reconstituting channels into planar membranes: a conceptual framework and methods for fusing vesicles to planar bilayer phospholipid membranes. Methods in Enzymology 220:50-68
16. 16. Cohen, F.S., Zimmerberg, J., Finkelstein, A. 1980. Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. II. Incorporation of a vesicular membrane marker into the planar membrane. J. Gen. Physiol. 75:251-270
17. 17. Düzgüneş, N., Allen, T.M., Fedor, J., Papahadjopoulos, D. 1987. Lipid mixing during membrane aggregation and fusion: why fusion assays disagree. Biochemistry 26:8435-8442
18. 18. Düzgünes, N., Goldstein, J.A., Friend, D.S., Felgner, P.L. 1989. Fusion of liposomes containing a novel cationic lipid, N-[2,3-(Dioleyloxy)propyl]-N,N,N,-trimethylammonium: induction by multivalent anions and asymmetric fusion with acidic phospholipid vesicles. Biochemistry 28:9179-9184
19. 19. Düzgüneş, N., Wilschut, J. 1993. Fusion assays monitoring intermixing of aqueous contents. Methods in Enzymology 220:3-14.
20. 20. Düzgüneş, N., Wilschut, J., Fraley, R., Papahadjopoulos, D. 1981. Studies on the mechanism of membrane fusion. Role of head-group composition in calcium-and magnesium-induced fusion of mixed phospholipid vesicles. Biochim. Biophys. Acta 642:182-195
21. 21. Ellens, H., Siegel, D.P., Alford, D., Yeagle, P.L., Boni, L., Lis, L.J., Quinn, P.J., Bentz, J. 1989. Membrane fusion and inverted phases. Biochemistry 28,3692-3703
22. 22. Evans, E.A. 1974. Bending resistance and chemically induced moments in membrane bilayers. Biophys. J. 14:923-931
23. 23. Evans, E.A., Parsegian, V.A. 1983. Energetics of membrane deformation and adhesion in cell and vesicle aggregation. Ann. N.Y. Acad. Sci. 416:13-33
24. 24. Franzin, C.M., MacDonald, P.M. 1997. Detection and quantification of asymmetric lipid vesicle fusion using deuterium NMR. Biochemistry 36:2360-2370
25. 25. Helfrich, W. 1998. Elastic properties of lipid bilayers: Theory and possible experiments. Z. Naturforsch 28:693-703
26. 26. Helm, C.A., Israelachvili, J.N., McGuiggan, P.M. 1992. Role of hydrophobic forces in bilayer adhesion and fusion. Biochemistry 31:1794-1805
27. 27. Hoekstra, D., Düzgüneş, N. 1993. Lipid mixing assays to determine fusion in liposome systems. Methods in Enzymology 220:15-32
28. 28. Hui, S.W., Stewart, T.P., Boni, L.T., Yeagle, P.L. 1981. Membrane fusion through point defects in bilayers. Science 212:921-923
29. 29. Kachar, B., Fuller, N., Rand, R.P. 1986. Morphological responses to calcium-induced interaction of phosphatidylserine-containing vesicles. Biophys. J. 50:779-788
30. 30. Kendall, D.A., MacDonald, R.C. 1982. A fluorescence assay to monitor vesicle fusion and lysis. J. Biol. Chem. 257:13892-13895
31. 31. Kendall, D.A., MacDonald, R.C. 1983. Characterization of a fluorescence assay to monitor changes in the aqueous volume of lipid vesicles. Anal. Biochem. 134:26-33
32. 32. Kozlov, M.M., Leikin, S.L., Chemomordik, L.V., Markin, V.S., Chizmadzhev, Y.A. 1989. Stalk mechanism of vesicle fusion. Intermixing of aqueous contents. European Biophysics Journal 17:121-129
33. 33. Kozlov, M.M., Markin, V.S. 1984. On the theory of membrane fusion. The adhesion-condensation mechanism. Gen. Physiol. Biophys. 5:379-402
34. 34. Kummrow, M., Helfrich, W. 1996. Collapse of giant phosphatidylcholine vesicles. Chem. Phys. Lipids 79:147-156
35. 35. Larsson, K. 1994. Lipids - Molecular Organization, Physical Functions and Technical Applications. The Oily Press, Dundee
36. 36. Leckband, D.E., Helm, C.A., Israelachvili, J. 1993. Role of calcium in the adhesion and fusion of bilayers. Biochemistry 32:1127-1140
37. 37. Lee, J., Lentz, B.R. 1997. Evolution of lipidic structures during model membrane fusion and the relation of this process to cell membrane fusion. Biochemistry 36:6251-6259
38. 38. Lentz, B.R. 1994. Polymer-induced membrane fusion: potential mechanism and relation to cell fusion events. Chem. Phys. Lipids 73:91-106
39. 39. MacDonald, R.C. 1988. Mechanisms of membrane fusion in acidic lipid-cation systems. In: Molecular mechanisms of Membrane Fusion. S. Ohki, D. Doyle, T.D. Flanagan, S.W. Hui, E. Mayhew, editors. pp. 101-112. Plenum Press, NY
40. 40. Needham, D., Haydon, D.A. 1983. Tensions and free energies of formation of "solventless" lipid bilayers. Measurement of high contact angles. Biophys. J. 41:251-257
41. 41. Niles, W.D., Cohen, F.S. 1987. Video Fluorescence microscopy studies of phospholipid vesicle fusion with a planar phospholipid membrane. Nature of membrane-membrane interactions and detection of release of contents. J. Gen. Physiol. 90:703-735
42. 42. Niles, W.D., Cohen, F.S. 1991. Video microscopy studies of vesicle-planar membrane adhesion and fusion. Ann. N. Y. Acad. Sci. 635:273-284
43. 43. Ohki, S., 1993. Fusion of spherical membranes. Methods in Enzymology 220:79-89
44. 44. Palade, G. 1975. Intracellular aspects of the process of protein synthesis. Science 189:347-358
45. 45. Papahadjopoulos, D, Nir, S., Düzgüneş, N. 1990. Molecular mechanisms of calcium-induced membrane fusion. Journal of Bioenergetics & Biomembranes 22:157-179
46. 46. Parsegian, V.A., Rand, R.P. 1983. Membrane interaction and deformation. Ann. N. Y. Acad. Sci. 416:1-12
47. 47. Perin, M.S., MacDonald, R.C. 1989. Interactions of liposomes with planar bilayer membranes. J. Membrane Biol. 109:221-232
48. 48. Rakhmanova, V.A., MacDonald, R.C. 1998. A microplate fluorimetric assay for transfection of the β-galactosidase reporter gene. Anal. Biochem. 257:234-237
49. 49. Rand, R.P. 1986. Mimicry and mechanism in phospholipid models of membrane fusion. Ann. Rev. Physiol. 48:201-212
50. 50. Rand, R.P., Kachar, B., Reese, T.S. 1985. Dynamic morphology of calcium-induced interactions between phosphatidylserine vesicles. Biophys. J. 47:483-489
51. 51. Rothman, J.E. 1996. The protein machinery of vesicle budding and fusion. Prot. Sci. 5:185-194
52. 52. Schekman, R., Orci, L. 1996. Coat proteins and vesicle budding. Science 271:1526-1533
53. 53. Siegel, D.P. 1986. Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal lipid phases. II. Implications for membrane-membrane interactions and membrane fusion. Biophys. J. 49:1171-1183
54. 54. Siegel, D.P. 1993. Energetics of intermediates in membrane fusion: comparison of stalk and inverted micellar intermediate mechanisms. Biophys. J. 65:2124-2140
55. 55. Stamatatos, L., Leventis, R., Zuckermann, M.J., Silvius, J.R. 1988. Interactions of cationic lipid vesicles with negatively charged phospholipid vesicles and biological membranes. Biochemistry 27:3917-3925
56. 56. Stegmann, T., Schoen, P., Bron, R., Wey, J., Bartoldus, I., Ortiz, A., Nieva, J.L., Wilschut, J. 1993. Evaluation of viral membrane fusion assays. Comparison of the octadecylrhodamine dequenching assay with the pyrene excimer assay. Biochemistry 32:11330-11337
57. 57. Struck, D.K., Hoekstra, D., Pagano, R.E. 1981. Use of resonance energy transfer to monitor membrane fusion. Biochemistry 20:4093-4099
58. 58. Verkleij, A.J. 1984. Lipidic intramembranous particles. Biochim. Biophys. Acta 779:43-63
59. 59. Walter, A., Yeagle, P.L., Siegel, D.P. 1994. Diacylglycerol and hexadecane increase divalent cation-induced lipid mixing rates between phosphatidylserine large unilamellar vesicles. Biophys. J 66:366-376
60. 60. White, J.M. 1992. Membrane fusion. Science 258:917-924
61. 61. Wilschut, J., Düzgüneş, N., Hong, K., Hoekstra, D., Papahadjopoulos, D. 1983. Retention of aqueous contents during divalent cation-induced fusion of phospholipid vesicles. Biochim. Biophys. Acta 734:309-318
62. 62. Wilschut, J., Düzgüneş, N., Papahadjopoulos, D. 1981. Calcium/ Magnesium specificity in membrane fusion: kinetics of aggregation and fusion of phosphatidylserine vesicles and the role of bilayer curvature. Biochemistry 20:3126-3133
63. 63. Wilschut, J., Hoekstra, D. 1986. Membrane fusion: Lipid vesicles as a model system. Chem. Phys. Lipids 40:145-166
64. 64. Woodbury, D.J. 1989. Pure lipid vesicles can induce channel-like conductances in planar bilayers. J. Membrane Biol. 109:145-150
65. 65. Woodbury, D.J., Hall, J.E. 1988. Role of channels in the fusion of vesicles with a planar bilayer. Biophys. J. 54:1053-1063
66. 66. Zhelev, D.V., Needham, D. 1993. Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension. Biochim. Biophys. Acta 1147:89-104
67. 67. Zschomig, O., Arnold, K., Richter, W., Ohki, S. 1992. Dextran sulfate-dependent fusion of liposomes containing cationic stearylamine. Chem. Phys. Lipids 63:15-22