Separation of nucleotide oligomers by unitary anion-exchange

Journal of Chromatographic Science

Volumn 37, Issue 7, 1999, Pages 251-254

  Lochmuller C H ^a   Liu, Qicai ^a   Huang, Lingyan ^b   Li, Ying ^a  

^a DUKE UNIVERSITY   (United States)

^b INTEGRATED DNA TECHNOLOGIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANION EXCHANGE RESIN; BIOPOLYMER; NUCLEOTIDE; SOLVENT;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; TEMPERATURE; ULTRAVIOLET SPECTROPHOTOMETRY;

ANION EXCHANGE RESINS; BASE SEQUENCE; BIOPOLYMERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; MOLECULAR SEQUENCE DATA; NUCLEOTIDES; SOLVENTS; SPECTROPHOTOMETRY, ULTRAVIOLET; TEMPERATURE;

EID: 0033161494     PISSN: 00219665     EISSN: None     Source Type: Journal    
DOI: 10.1093/chromsci/37.7.251     Document Type: Article

References (10)

1. D. Rickwood and B.D. Hames. Gel Electrophoresis of Nucleic Acids: a Practical Approach, 2nd ed. IRL Press, New York, NY, 1990.
2. A.M. Delort, R. Derbyshire, A.M. Duplaa, A. Guy, D. Molko, and R. Teoula. Use of reversed-phase high-performance liquid chromatography for the purificaiton of 5′-phosphorylated oligonucleotides. J. Chromatogr. 283: 462-67 (1984).
3. 4 column for reversed-phase high-performance liquid chromatographic purification of synthetic oligonucleotides. J. Chromatogr. 326: 293-99 (1985).
4. W. Haupt and A. Pingoud. Comparison of several high-performance liquid chromatography techniques for the separation of oligodeoxynucleotides according to their chain length. J. Chromatogr. 260: 419-27 (1983).
5. Y. Kato, T. Kitamura, A. Mitsui, Y. Yamasaki, and T. Hashimoto. Separation of oligonucleotides by high-performance ion-exchange chromatography on a non-porous ion exchanger. J. Chromatogr. 447: 212-20 (1988).
6. A.A. Kurganov, V.A. Davankov, and K.K. Unger. Ion-exchange high-performance liquid chromatography of nucleotides and polypeptides on new types of ion-exchange sorbents, based on polystyrene-coated silica. J. Chromatogr. 548: 207-14 (1991).
7. T. Lu and H.B. Gray. Combined pH gradient and anion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotides. J. Chromatogr. A 686: 333-38 (1994).
8. W.J. Warren, W.A. Stanick, M.V. Gorenstein, and P.M. Young. HPLC analysis of synthetic oligonucleotides using spectral contrast techniques. Biotechniques 19: 282-87 (1995).
9. W.A. Ausserer and M.L. Biros. High-resolution analysis and purification of synthetic oligonucleotides with strong anion-exchange HPLC. Biotechniques 19: 136-39 (1995).
10. J.L. Vaerman, P. Moureau, Y. Larondelle, P. Martiat, and M. Philippe. Prediction of oligodeoxyribonucleotides eluting salt conditions on anion exchange column. Nucleic Acids Research 20: 3522 (1992).