Purification of bovine S100A12 from recombinant Escherichia coli

Protein Expression and Purification

Volumn 16, Issue 1, 1999, Pages 47-52

  Yamashita, Kayoko ^a   Oyama, Yuhta ^a   Shishibori, Tsuyoshi ^a   Matsushita, Osamu ^a   Okabe, Akinobu ^a   Kobayashi, Ryoji ^a  

^a KAGAWA UNIVERSITY   (Japan)

Author keywords

Antiallergy drug; Calcium binding protein; Drug affinity chromatography; Overexpression; Recombinant protein; S100 family protein; T7 RNA polymerase

Indexed keywords

AMINO ACID SEQUENCE; CALCIUM BINDING PROTEIN; EDETIC ACID; ESCHERICHIA COLI; ION EXCHANGE CHROMATOGRAPHY; LYSYLENDOPEPTIDASE; PROTEIN PURIFICATION;

BOVINAE; ESCHERICHIA COLI;

EID: 0033153513     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.1026     Document Type: Article

References (18)

1. Pedrocchi, M., Schäfer, B. W., Durussel, I., Cox, J. A., and Heizmann, C. W. (1994) Purification and characterization of the recombinant human calcium-binding S100 proteins CAPL and CACY. Biochemistry 33, 6732-6738.
2. Schäfer, B. W., and Heizmann, C. W. (1996) The S100 family of EF-hand calcium-binding proteins: Functions and pathology. Trends Biochem. Sci. 21, 134-140.
3. Baudier, J., Delphin, C., Grunwald, D., Khochbin, S., and Lawrence, J. J. (1992) Characterization of the tumor suppressor protein p53 as a protein kinase C substrate and a S100b-binding protein. Proc. Natl. Acad. Sci. USA 89, 11627-11631.
4. Donato, R. (1991) Perspectives in S-100 protein biology: Review article. Cell Calcium 12, 713-726.
5. Okazaki, K., Niki, I., Iino, S., Kobayashi, S., and Hidaka, H. (1994) A role of calcyclin, a Ca (2+)-binding protein, on the Ca (2+)-dependent insulin release from the pancreatic beta cell. J. Biol. Chem. 269, 6149-6152.
6. Timmons, P. M., Chan, C. T., Rigby, P. W., and Poirier, F. (1993) The gene encoding the calcium binding protein calcyclin is expressed at sites of exocytosis in the mouse. J. Cell. Sci. 104, 187-196.
7. Ilg, E. C., Troxler, H., Bürgisser, D. M., Kuster, T., Makert, M., Guignard, F., Hunziker, P., Birchler, N., and Heizmann, C. W. (1996) Amino acid sequence determination of human S100A12 (P6, calgranulin C, CGRP, CAAF1) by tandem mass spectrometry. Biochem. Biophys. Res. Commun. 225, 146-150.
8. Guignard, F., Mauel, J., and Markert, M. (1995) Identification and characterization of a novel human neutrophil protein related to the S100 family. Biochem. J. 309, 395-401.
9. Dell'Angelica, E. C., Schleicher, C. H., and Santomé, J. A. (1994) Primary structure and binding proteins of cargranulin C, a novel S100-like calcium-binding protein from pig granulocytes. J. Biol. Chem. 269, 28929-28936.
10. Hitomi, J., Yamaguchi, K., Kikuchi, Y., Kimura, T., Maruyama, K., and Nagasaki, K. (1996) A novel calcium-binding protein in amniotic fluid, CAAF1: Its molecular cloning and tissue distribution. J. Cell. Sci. 109, 805-815.
11. Yamaguchi, T., Hitomi, J., Nagasaki, K., Suzuki, M., Takahashi, E., Saito, S., Tsukada, T., and Yamaguchi, K. (1996) Human CAAF1 gene-molecular cloning, gene structure, and chromosome mapping. Biochem. Biophys. Res. Commun. 221, 356-360.
12. Wicki, R., Marenholz, I., Mischke, D., Schäfer, B. W., and HeizMann, C. W. (1996) Characterization of the human S100A12 (cargranulin C, p6, CAAF1, CGRP) gene, a new member of the S100 gene cluster on chromosome 1q21. Cell Calcium 20, 459-464.
13. Oyama, Y., Shishibori, T., Yamashita, K., Naya, T., Nakagiri, S., Maeta, H., and Kobayashi, R. (1997) Two distinct anti-allergic drugs, amlexanox and cromolyn, bind to the same kinds of calcium binding proteins, except calmodulin, in bovine lung extract. Biochem. Biophys. Res. Commun. 240, 341-347.
14. van den Bos, C., Rammes, A., Vogl, T., Boynton R., Zaia, J., Sorg, C. and Roth, J. (1998) Copurification of P6, MRP8, and MRP14 from human granulocytes and separation of individual proteins. Protein Express. Purif. 13, 313-318.
15. Schägger, H., and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.
16. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
17. Frenandez, J., DeMott, M., Atherton, D., and Mische, S. M. (1992) Internal protein sequence analysis: Enzymatic digestion for less than 10 μg of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal. Biochem. 201, 255-264.
18. Shishibori, T., Oyama, Y., Matsushita, O., Yamashita, K., Furuichi, H., Okabe, A., Maeta, H., Hata, Y., and Kobayashi, R. (in press) Biochem. J.